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<p><b>OBJECTIVE</b>To detect the presence of p15 antisense RNA(p15AS) in acute myeloid leukemia(AML).</p><p><b>METHODS</b>p15AS and p15 mRNA in two leukemia cell lines was detected with strand-specific primer RT-qPCR. To explore the connection between p15AS and AML, 43 patients with newly diagnosed AML and 21 patients with benign diseases (Iron deficiency anemia) as controls were enrolled. The expression level of p15AS in bone marrow cells derived from the patients and the controls were determined by strand-specific primer RT-qPCR, and its relationship with clinical features was analyzed.</p><p><b>RESULTS</b>The two AML lines displayed high p15AS and low p15 expression. Samples derived from the AML patients showed relatively increased expression of p15AS and down-regulated p15 expression in their bone cells. In contrast, the 21 controls showed high expression of p15 but relatively low expression of the p15AS. Compared with the normal controls, the expression levels of p15 protein were significantly lower among the AML patients (P<0.01). No relationships were detected between the level of p15AS and the patient's age, gender, FAB subtype, total white blood cell count, platelet count, proliferative degree of bone marrow cell and karyotype classification (P>0.05 for all comparisons).</p><p><b>CONCLUSION</b>High expression of p15 antisense RNA was frequently found among AML patients, and may play an important role in epigenetic silencing of p15.</p>
Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Células de la Médula Ósea , Metabolismo , Inhibidor p15 de las Quinasas Dependientes de la Ciclina , Genética , Epigénesis Genética , Regulación Neoplásica de la Expresión Génica , Leucemia Mieloide Aguda , Genética , Metabolismo , ARN sin Sentido , Genética , Metabolismo , Regulación hacia ArribaRESUMEN
AIM:To analyze circulating miR-141 in the serum as a non-invasive biomarker in the patients with prostate cancer ( PCa) and benign prostate hyperplasia ( BPH) , and healthy individuals.METHODS: A total of 75 pa-tients with PCa, 52 with BPH and 40 healthy individuals were enrolled into this study.Total RNA was isolated from the se-rum samples and the circulating levels of miR-141 were determined using quantitative real-time polymerase chain reaction. RESULTS:The serum levels of miR-141 were significantly higher in the patients with PCa compared to the patients with BPH and the healthy controls (P0.05).The serum miR-141 level was also found to be related to Gleason score, clinical stage and bone me-tastasis status of the patients with PCa (P0.05 for all comparisons).CONCLUSION: Circulating miR-141 could serve as a non-invasive biomarker for prostate cancer diagnosis, staging and prognosis prediction.
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Objective To study the morphology changes and the expression of phosphatidyl serine(PS) in apheresis platelets(Plts) during storage,also to analyze their relationship,in order to provide laboratorial evidence for the in vitro storage period of plts.Methods The morphological changes of plts during 8-day storage were detected by May-Grunwald-Giemsa dyeing method,and the expression of PS on platelet membrane was tested by flow cytometry(FCM).Results The morphological lesions appeared on the fourth day of storage,because the modified morphological score(MMS) of 4-day group was significantly lower than that of fresh plts(t=2.341,P