Research of cellular toxic effect to Hep-2 of recombinant toxin MSH-Ang / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the cytotoxicity of recombinate toxin MSH-Ang to Hep-2.@*METHOD@#The depurated MSH-Ang were applied in cytotoxicity experiment, and the growth inhibiting action to laryngeal carcinoma cell Hep-2 were observed.@*RESULT@#Recombination protein inhibited the growth of laryngeal carcinoma cell Hep-2, and its inhibiting action enhanced and corpuscular mortality rate increased along with the concentration increasing.@*CONCLUSION@#Recombinant toxin MSH-Ang can not only take special effect in tumors with high MSHR, but also target to many other popular tumors.

Application of coblation assisted upper-airway procedure to obstructive sleep apnea-hypopnea syndrome / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the efficacy and the value of CAUP in treatment of OSAHS.@*METHOD@#CAUP and the dissection of palatopharyngeus muscle were performed by RFVTR . One hundred and sixty-eight patients with OSAHS treated by CAUP from July 2001 to July 2004 were summarized. Among them, 52 patients were analyzed by PSG after 1 year of the operation.@*RESULT@#All the operative procedure were carried out smoothly with excellent patient tolerance. Post-operation complications were foreign body feeling in oral cavity. No velopalatal insufficiency occurred. The efficient rate was 94.2% after 1 year of the operation according to the PSG results.@*CONCLUSION@#CAUP is a simple, safe,repeatable and acceptable surgical procedure and it was developing with excellent value in the treatment of OSAHS on the basic of accurate X-ray imaging.

Expression and distribution of aquaporin 1 in laryngeal carcinoma / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To detect expression level and distribution of aquaporin 1 (AQP1) in laryngeal carcinoma tissues and try to find out the significance about the mechanism of AQP1 in the invasion of laryngeal carcinoma.@*METHOD@#Twenty cases of laryngeal carcinoma tissues and 15 cases of normal tissues beside tumors were acquired immediately after surgery. The expression and distribution of aquaporin 1 in laryngeal carcinoma were examined by RT-PCR, western blot analysis and immunohistochemical technique.@*RESULT@#AQP1 expresses on the capillary endothelia of laryngeal mucous lamina propria and laryngeal mucous glands of normal tissues and mainly on the capillary endothelia cells, tumor cells and cancer nests of laryngeal carcinoma tissues. AQP1 mRNA and protein expression level in laryngeal tumor tissues is remarkably stronger than that in normal tissues beside tumors.@*CONCLUSION@#The expression of AQP1 was increased in laryngeal carcinoma tissues over normal laryngeal tissues. It suggests that the mechanism of AQP1 in laryngeal carcinoma etiopathogenesis needs to be studied.

Experience in treating anterior-lateral benign tumor in middle cranial fossa base through standard facial translocation approach / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To observe the feasibility and effect of improved standard facial translocation approach in treating anterior-lateral benign tumor in middle cranial fossa base.@*METHOD@#We excised 10 cases of benign tumor originating from pharynx nasals of pterygopalatine fossa and expanding toward the cavernous sinus of middle cranial fossa, infratemporal fossa or pterygo-maxillary space. We ameliorated, facial incision, maxilla disassembled, reestablishment, fixation,maxillary mucosal option and remained lateral wall of nasal cavity to some extent.@*RESULT@#The mean time of following-up was 38 month postoperatively without complications of bleeding, infection and necrosis or prolapse of displacing bones. Nine patients were treated successfully, one died.@*CONCLUSION@#Reforming facial translocation approach in treating anterior-lateral benign tumor in middle cranial fossa base was safe and obtained ideal curative effect in near future.

Protein kinase C in proliferation and infiltration of eosinophils in nasal polyp / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To explore the significance of protein kinase C (PKC) in proliferation and infiltration of eosinophils in nasal polyps.</p><p><b>METHODS</b>With in situ hybridization and immunohistochemistry staining methods, PKC, pro-apoptotic, and anti-apoptotic gene (Bax, bcl-2) expressions were measured in nasal polyp tissues from 26 patients and inferior turbinate mucosa tissues (ITMTs) from 20 healthy persons. The May-Grünwald-Giemsa (MGG) staining method was used to identify eosinophils.</p><p><b>RESULTS</b>In eosinophils, the positive cell expressive rates of Bcl-2 mRNA and its protein were significantly higher in the group with nasal polyps than in the ITMT group (P < 0.01). Although the positive cell expressive rate of Bax mRNA and associated protein were a little higher in the group with nasal polyp tissues than in the ITMT group, the difference was not significant (P > 0.05). There was PKC expression in the eosinophils of 26 cases of nasal polyps, but occasional PKC expression in 7 of 20 ITMT cases. In the two groups, PKC positive cell expression was significantly different, and the expression of PKC and bcl-2 mRNA as well as associated protein in eosinophils of nasal polyps showed a remarkably positive relationship (r1 = 0.0875, r2 = 0.0823, P < 0.01).</p><p><b>CONCLUSIONS</b>Increased PKC expression in eosinophils of nasal polyp tissues is closely associated with apoptosis inhibition, and it is presumed that eosinophil apoptosis inhibition in nasal polyp tissues is obtained by activation of the PKC signal transduction pathway.</p>

Local tissue hypoxia and formation of nasal polyps / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To explore the response of nasal mucosa epithelial cells to hypoxia in terms of formation of nasal polyps (NP).</p><p><b>METHODS</b>Epithelial cells of NP and inferior turbinate (IT) were cultured serum-free under normal oxygen and hypoxic circumstances with stimulation of IL-1 beta and TNF alpha. The vascular endothelial growth factor (VEGF) mRNA and VEGF protein levels of the cultured cells were detected using in situ hybridization and ELISA, respectively.</p><p><b>RESULTS</b>The expression of VEGF mRNA was significantly higher in epithelial cells of NP than in IT exposed to pro-inflammatory cytokines or hypoxia (P < 0.01). VEGF levels were higher in NP epithelial cells than those of IT (P < 0.01) under hypoxia.</p><p><b>CONCLUSION</b>VEGF-induced by hypoxia is very important for the early stages of forming polyps.</p>

Expression of TNF\|α and CD68 in nasal polyps tissue and their significance / 中国耳鼻咽喉头颈外科

Objective: To study the expression of TNF\|α and CD68 in nasal polyps tissue and their significance. Methods. Paraffin sections of 19 nasal polyps and 8 chronic rhinitis samples were studied with immunohistochemical technique. Results. The expression of TNF\|α were stronger in nasal polyps tissues than that in controls. Numerous CD68 positive cells were found in epithelium laryer and in the lamina propria of nasal polyps. There were positive relationship between the expression of TNF\|α and that of CD68(r=0.615). Conclusion. It was suggested that activized macrophage as antigen\|presenting cell in the development of nasal polyps, may play a important role through upregulated TNF\|α′s synthesis and secretion.

The impact of lateral rhinotomy on nasal airway / 临床耳鼻咽喉头颈外科杂志

Objective:In order to ascertain the lateral operation and medial maxillectomy affect on nasal airway.Method:Report 16 cases of nasal cavity or nasal sinus neoplasm who were therapied by lateral rhinotomy and medial maxillectomy.They were observed by acoustic rhinometer (AR) and CT examination in 1～2.5 years after the surgery. Result:The feeling of nasal obstruct were disappeared in 3 months after the surgery. The nasal airway resistance(NAR) of the ill nasal cavity was markedly decreased after the surgery, and the total NAR were decreasing unmarkably. The mucous proliferation was notable only in the first year after surgery, and especially on remains of the inferior turbinate or extra wall of nasal cavity.Conclusion:Lateral rhinotomy operation and medial maxillectomy have less impact on nasal airway and the functions.

Influence of anisodamin on guinea pig experimental nasal hypersensitivity / 临床耳鼻咽喉头颈外科杂志

Objective:To observe the influence of anisodamin on guinea pig experimental nasal hypersensitivity.Method:Experimental animals were divided into normal control group, TDI group,reserpine group and anisodamine group.The scores of symptom of nose and the cytologic changes of nasal mucosa were parameters of observing.Result:The nasal symptom of reserpine group was significantly stronger than the TDI group,and the eosinophilia evidently increased (both P＜0.01).The nasal symptom of the anisodamine group reduced and the eosinophilia decreased.It is significantly difference between the TDI group and the reserpine group (both P＜0.01).Conclusion:Anisodamine may play a role in therapy of the experimental nasal hypersensitivity with hyperfuction of parasympathetic nerve.

Nasal cavity volume and nasopharyngeal cavity volume in adults measured by acoustic rhinometry / 临床耳鼻咽喉头颈外科杂志

Objective:To investigate the range of normal value of total nasal cavity volume(NV)and total na-sopharyngeal cavity volume (NPV)of healthy adults. Method: Six hundred and fifty-nine healthy adults andeighty-two adults of chronic rhinitis were measured with acoustic rhinometry. Result:The range of NV was29. 922～37. 481 cm3 and NPV was 29. 369～44. 159 cm3. Comparing healthy adults with adults of chronic rhini-tis,there was a significant difference in NV. Conclusion:Acoustic rhinometry suited for objective assessment ofthe nasal airways in adults. It was demonstrated that these data could provide available information for the studyof nasal physiology and pathophysiology,as well as for the diagnosis and judgement of therapeutic effectiveness ofnasal diseases.

The expression and effect of IL-4 in human palatine tonsil / 中国病理生理杂志

AIM: To study the allergic disease in recurrent tonsillitis. METHODS: Immunohistochemical technique was used to investigate interleukin-4(IL-4) production in tonsil. RESULTS:The result showed that: a higher incidence of IL-4 producing cells were found in the tonsils of all ages, and a high density of IL-4 was found in the reticular crypt epithelium and extrafollicular, but low density in germinalcenter. IL-4 has been shown play a crucial role in the pathogensis of allergic disease. CONCLUSION:Allergic disease is an important reason of recurrent tonsillitis. The conclusion will give a new way to cure recurrent tonsillitis.