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@#Objective To investigate the effect of silencing E6-associated protein(E6AP)on the level of p53 protein in human papilloma virus(HPV)negative cervical cancer cells(C33A cells).Methods The siRNA sequence silencing E6AP(siE6AP)and silencing control disordered siRNA sequence(siControl)were transfected into C33A cells with the mediation of LipofectamineTM2000 transfection reagent respectively.The silencing effect of siRNA on E6AP and the expression of p53and cleaved-caspase-3 proteins were detected by Western blot.Results The levels of E6AP protein in C33A cells of siE6AP group were significantly lower(t =-4.597,P<0.05),while the levels of p53 and cleaved-caspase-3 proteins were significantly higher than those of siControl group(t = 4.533 and 7.099 respectively,each P<0.05).Conclusion Silencing of E6AP significantly increased the expression of p53 protein in C33A cells,indicating that silencing of E6AP may restore the activity and function of p53 protein in C33A cells.
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Objective@#To understand the willingness and associated factors with novel coronavirus vaccination (‘COVID-19 vaccination’) among parents of primary and middle school students in Tongzhou District of Beijing, and to provide reference for studying the feasibility of COVID-19 vaccination among students.@*Methods@#Multistage stratified cluster random sampling method was conducted to select 3 026 parents of students in Tongzhou District. An online questionnaire survey was conducted to compare the willingness of COVID-19 vaccination by different characteristics. Multivariate Logistic regression model was conducted to analyze associated factors of COVID-19 vaccination.@*Results@#About 65.40% of parents were willing to vaccinate their children. Multivariate Logistic regression analysis showed that parets from rural areas, who had daughters, children in good health, who direct participated in the prevention and control, and those whose child had received vaccines at their own cost are more positive with COVID-19 vaccination of children(OR=1.17, 1.33, 0.64, 1.32, 1.47, P<0.05).@*Conclusion@#Parents of primary and secondary schools in Tongzhou District have a low willingness to vaccinate their children with COVID-19 vaccine. The propaganda of the government authorities and the official media should be strengthened, and the vaccination rate should be improved through the recommendation of medical personnel.
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Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific identification target to develop a novel hybridization signal amplification method (HSAM) for the rapid and direct detection of MTBC from clinical sputum specimens. This system consists of magnetic bead-linked capture probes for target isolation, dextranbased nanoparticles for amplifying the reporter molecule (biotinylated-FITC), and detection probes (2B-DNA) for binding the nanoparticles. Both the capture and detection probes were specific to the IS6110 target sequence. Our results determined that as few as 10 copies of the IS6110 sequence or 10 M. tuberculosis bacteria could be detected, indicating that the HSAM assay is as sensitive as conventional PCR, and the assay was specific enough to distinguish MTBC from nontuberculosis mycobacteria (NTM). A total of 176 clinical sputum specimens were collected for HSAM evaluation, and the results were compared to those from traditional culture and biochemical identification techniques. This assay had a sensitivity of 88.3 percent, a specificity of 91.8 percent, a positive predictive value of 93.8 percent and a negative predictive value of 84.8 percent for the detection of MTBC. This technique is highly sensitive and specific, is easy to perform, and does not require any sophisticated detection equipment; thus, this approach has great potential in clinical TB detection and diagnostic applications.