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Chinese Journal of Natural Medicines (English Ed.) ; (6): 24-29, 2014.
Artículo en Inglés | WPRIM | ID: wpr-812313

RESUMEN

AIM@#To investigate the anticancer activity of DT-13 under normoxia and determine the underlying mechanisms of action.@*METHODS@#MDA-MB-435 cell proliferation, migration, and adhesion were performed to assess the anticancer activity of DT-13, a saponin from Ophiopogon japonicus, in vitro. In addition, the effects of DT-13 on tumor growth and metastasis in vivo were evaluated by orthotopic implantation of MDA-MB-435 cells into nude mice; mRNA levels of vascular endothelial growth factor (VEGF), C-C chemokine receptor type 5 (CCR5) and hypoxia-inducible factor 1α (HIF-1α) were evaluated by real-time quantitative PCR; and CCR5 protein levels were detected by Western blot assay.@*RESULTS@#At 0.01 to 1 μmol·L(-1), DT-13 inhibited MDA-MB-435 cell proliferation, migration, and adhesion significantly in vitro. DT-13 reduced VEGF and CCR5 mRNAs, and decreased CCR5 protein expression by down-regulating HIF-1α. In addition, DT-13 inhibited MDA-MB-435 cell lung metastasis, and restricted tumor growth slightly in vivo.@*CONCLUSION@#DT-13 inhibited MDA-MB-435 cell proliferation, adhesion, and migration in vitro, and lung metastasis in vivo by reducing VEGF, CCR5, and HIF-1α expression.


Asunto(s)
Animales , Femenino , Humanos , Ratones , Antineoplásicos Fitogénicos , Neoplasias de la Mama , Quimioterapia , Genética , Metabolismo , Antagonistas de los Receptores CCR5 , Adhesión Celular , Línea Celular Tumoral , Movimiento Celular , Medicamentos Herbarios Chinos , Liriope (Planta) , Química , Ratones Desnudos , Tubérculos de la Planta , Química , Receptores CCR5 , Genética , Metabolismo , Saponinas , Factor A de Crecimiento Endotelial Vascular , Genética , Metabolismo
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