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Objective@#To investigate the effectiveness of horticultural therapy on improving university students mental health problems and to provide evidence to support the application of horticultural therapy in the prevention and treatment of university students mental health.@*Methods@#From March to May 2022,a before-and after paired design was used with a blank control group, mental health screening abnormalities were recruited from a comprehensive university in Hebei Province, including 57 in the intervention group and 21 in the control group, and the Symptom Checklist-90(SCL-90) score and hair cortisol were included as outcome indicators, which was measured by enzyme linked immunoassay Elisa double antibody sandwich method.@*Results@#The total SCL-90 scores of the intervention group decreased ( t=4.28, P <0.01) and were significantly lower compared with the control group( t=-2.66, P <0.01), while no significant difference was observed in the control group( t=0.29, P >0.05), and the difference between the pre and post measures on each dimension of the SCL-90 was significantly lower in the intervention group (all P <0.01); the difference between the pre and post measures of hair cortisol contentration in the intervention group was statistically significant( Z= -2.75, P <0.05), and the hair cortiso contentration in pre measure group was lower than that of control group( Z=-3.48, P < 0.01 ). In contrast, the difference between pre and post measurements of hair cortisol in the control group was not significant( Z= -0.75 , P >0.05).@*Conclusion@#Horticultural therapy can improve the psychological symptoms of university students is suitable for psychological interventions in the university population and is beneficial to the health and well being of university students.
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OBJECTIVE: To isolate four polysaccharides from Tremella and study their physicochemical properties, microstructures, antioxidant activities and anti-inflammation effects. METHODS: The polysaccharides were purified using water extraction method, DEAE-Sepharose CL-6B and Sephadex G-100. The physicochemical properties and microstructures were studied using high-performance size-exclusion chromatography (HPSEC), gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analysis. Anti-inflammation activities of the polysaccharides were identified using real time quantitative PCR. RESULTS: Four kinds of major polysaccharides (TABW-Ⅱ, TFBW-Ⅱ, TEPW-Ⅱ and TSPW-) were purified from water-extraction crude polysaccharides from Tremella. TABW-Ⅱ, TFBW-Ⅱ, TEPW-Ⅱ and TSPW- were homogeneous with relative molecular mass 26×103, 11×103, 21×103 and 356×103, respectively, and they consisted mainly of glucose and mannose. TEM reveale that their molecular morphologies were linear, and TSPW- had more extended structure with entangled chains. Compared with vitamin C (Vc), TSPW- showed more noticeable scavenging effects of superoxide (EC50=0.127 mg•mL-1) and hydroxyl (EC50=0.088 mg•mL-1) radicals (P<0.05). TSPW- also showed the highest inhibitory effects of TNF-α, IL-6 and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 mouse macrophage cells, and the inhibitory effects were more than 95% at 150 μg•mL-1 TSPW-. CONCLUSION: The results indicate that TSPW- with entangled and extended liner structures have more obvious antioxidant and anti-inflammation effects than other major polysaccharides from Tremella family. Furthermore, this study indicates the potential utilization of Tremella polysaccharides in anti-inflammatory agents.