RESUMEN
OBJECTIVE@#To explore the protective and therapeutic effects of Faecalibacterium prausnitzii (Fp) supernatant on ulcerative colitis (UC) in mice induced by dextran sulfate sodium (DSS) and the underlying mechanisms. @*METHODS@#Forty male mice were randomly divided into a control group, a model group, a treatment group and a prevention group (n=10 in each group). The colorectal histopathologic damage score (HDS) were calculated; the proportion of helper T cell (Th17) in mononuclear cells (MNC) in spleen, the levels of IL-17A and IL-6 in plasma were detected; the mRNA levels of transcription factor retinoic acid-related orphan receptor-γt (ROR-γt), interleukin (IL)-17A and IL-6 in colon mucosa tissues were also determined. @*RESULTS@#Compared with the model group, the colon HDS in the treatment group and the prevention group were significantly decreased (both P0.05). The proportion of Th17 cells in spleen in the treatment group and the prevention group was also remarkably lower than that in the model group (both P0.05). @*CONCLUSION@#Fp supernatant has protective and therapeutic effects on ulcerative colitis in mice induced by DSS, which might be mediated by decrease of Th17 and IL-17A levels in the plasma and the colon mucosa tissues. Fp supernatant also can decrease mice colitis by reducing IL-6 levels.
Asunto(s)
Animales , Masculino , Ratones , Clostridiales , Colitis Ulcerosa , Alergia e Inmunología , Medios de Cultivo Condicionados , Farmacología , Sulfato de Dextran , Interleucina-17 , Sangre , Alergia e Inmunología , Interleucina-6 , Sangre , Alergia e Inmunología , Mucosa Intestinal , Alergia e Inmunología , Miembro 3 del Grupo F de la Subfamilia 1 de Receptores Nucleares , Metabolismo , Distribución Aleatoria , Células Th17 , Alergia e InmunologíaRESUMEN
AIM: To establish the method of determining procyanidin B2 content in grape seed extract by HPLC. METHODS: The determination was carried out with ZORBAX SB-C_ 18 column. The mobile phase consisted of acetonitrile-2% acetate buffer under the gradient elution condition, detection wavelength was at 280nm. RESULTS: There was a good linear relationship between the peak area and concentration in the range of 1~30 ?g/mL for procyanidin B2. The average of procyanidin B2 (n=5) was 99.29% and RSD=1.64%, respectively. CONCLUSION: The method is simple, accurate, reproducible and can be used for assay of procyanidin B2.