Preparation and in vitro evaluation of brucine-loaded polylacticacid nanoparticles / 中国中药杂志

<p><b>OBJECTIVE</b>To prepare and evaluate brucine-loaded polylacticacid nanoparticles (Bru-PLA-NPs).</p><p><b>METHOD</b>The Bru-PLA-NPs were prepared by solvent diffusion method. The physical, chemical properties and in vitro release behavior of the prepared Bru-PLA-NPs were evaluated, respectively.</p><p><b>RESULT</b>The mean particle size of the prepared Bru-PLA-NPs was 95 nm with polydispersity index of 0.362. The zeta potential was -15.68 mV. The mean loading and entrapment efficiency of Bru were 7% and 37%, respectively. Compared with Bru solution, an obvious sustained release behavior of Bru from Bru-PLA-NPs was observed in the in vitro release experiment.</p><p><b>CONCLUSION</b>The Bru-PLA-NPs prepared by solvent diffusion method exhibit small particle size, high Bru-loading efficiency, and obvious sustained release in vitro</p>

Determination of Cobalt in Urine by Graphite Furnace Atomic Absorption Spectrometry / 环境与健康杂志

Objective To develop a method for the determination of cobalt in urine by graphite furnace atomic absorption spectrometry(GFAAS).Methods Usingthe matrixmodifier tofind the best condition byimprovingthe cineration temperature and reducing the interference of gas phase in atomization stage.Results The best cineration temperature and atomization temperature was 1 200 ℃and 2 450 ℃respectively,taking15% PdCl2,NH3H2PO4 and HNO3 as the matrix modifier.The background absorb value was less than 0.05.The linear range was 0～75 ?g/L,the correlation coefficient was over 0.999,the average recovery rate was 97.2%,the relative standard deviations were 3.5%～6.5%,the detection limit was 1.5 ?g/L.The result of 16 urine samples determined with the present method showed no significant difference compared with that by using polarography.Conclusion This method is sensitive,accurate,simple and is applicable tothe determination ofcobalt in urine.