Construction of recombinant expression vector and prokaryotic expression of human epididymal sperm protein P34H / 中华男科学杂志

<p><b>OBJECTIVE</b>To acquire purified recombinant human epididymal sperm protein P34H for basic and clinical studies.</p><p><b>METHODS</b>On the basis of cloning of P34H coding region, P34H fragment was subcloned into the pQE-30 expression vector. The recombinant expression vector designated pQE-30/P34H was transformed into E. coli to induce the expression of the recombinant protein P34H on the reduction of IPTG. After sonication, the recombinant protein P34H was purified from the supernatant with Ni-NTA resin under native conditions. It was identified by SDS-PAGE analysis and DNA sequencing.</p><p><b>RESULTS</b>Recombinant expression vector pQE-30/P34H was correctly constructed, identified with PCR and double-enzyme digestion. And the results of SDS-PAGE analysis and DNA sequencing showed that the protein was what we had hoped to acquire.</p><p><b>CONCLUSION</b>Purified recombinant P34H can be acquired successfully with the above mentioned prokaryotic expression method.</p>

Investigation of STD/AIDS related KABP among the high-risk population / 中华男科学杂志

<p><b>OBJECTIVE</b>To understand the knowledge, attitude, behavior and perception (KABP) related to STD/AIDS among the high-risk population and to explore effective methods for health education and behavioral intervention.</p><p><b>METHODS</b>Seven hundred and twenty of the reeducated in a reeducation center were surveyed using questionnaires, which were collected and analyzed.</p><p><b>RESULTS</b>The knowledge of the subjects about STD/AIDS was insufficient. When they had sex with their clients, the preventive measures against STD/AIDS were incorrect and the proportion of condom use was small. And when infected with STD/AIDS, they turned to private clinics for nonstandard treatment.</p><p><b>CONCLUSION</b>The knowledge of save sex and the preventive measures against STD/AIDS need to be improved, and correct guidance is imperative as to the seeking of standard medical help among the high-risk population. The key to sexual health education was to establish a corresponding social supporting system.</p>