Factors and Their Impact on Treatment Effect of Acupuncture in Different Outcomes: A Meta-Regression of Acupuncture Randomized Controlled Trials / 中国结合医学杂志

BACKGROUND@#The effects of acupuncture have varied in different randomized controlled trials (RCTs), and there are many factors that influence treatment effect of acupuncture in different outcomes, with conflicting results.@*OBJECTIVE@#To identify factors and their impact on the treatment effect of acupuncture in different outcomes.@*METHODS@#Acupuncture RCTs were searched from 7 databases including Medline (PubMed), Embase, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, Wanfang Database, VIP Database, and China Biology Medicine disc between January 1st, 2015 and December 31st, 2019. Eligible studies must compare acupuncture to no acupuncture, sham acupuncture, or waiting lists, and report at least 1 patient-important outcome. A multi-level meta-regression was conducted using a 3-level robust mixed model and univariate analyses were performed for all independent variables, even those excluded from the multivariable model due to collinearities. We used thresholds of 0.2 and 0.4 for the difference of standardized mean differences (SMDs), categorising them as small (<0.2), moderate (0.2-0.4), or large (>0.4) effects.@*RESULTS@#The pain construct analysis involved 211 effect estimates from 153 studies and 14 independent variables. High-frequency acupuncture treatment sessions produced larger effects compared to low-frequency sessions [large magnitude, the difference of adjusted SMDs 0.46, 95% confidence interval (CI) 0.07 to 0.84; P=0.02]. The non-pain symptoms construct analysis comprised 323 effect estimates from 231 studies and 15 independent variables. Penetrating acupuncture showed moderately larger effects when compared to non-penetrating acupuncture (0.30, 95% CI 0.06 to 0.53; P=0.01). The function construct analysis included 495 effect estimates from 274 studies and 14 independent variables. Penetrating acupuncture and the flexible acupuncture regimen showed moderately larger effects, compared to non-penetrating acupuncture and fixed regimen, respectively (0.40, 95% CI 0 to 0.80; P=0.05; 0.29, 95% CI 0.06 to 0.53; P=0.01).@*CONCLUSIONS@#High-frequency acupuncture sessions appear to be a more effective approach to managing painful symptoms. Penetrating acupuncture demonstrated greater effect in relieving non-painful symptoms. Both penetrating acupuncture type and flexible acupuncture regimen were linked to significant treatment effects in function outcomes. Future studies should consider the factors that are significantly associated with the effects of acupuncture in patient-important outcomes.

The Mechanism of Nitric Oxide in Delaying Post-harvest Senescence of Agricultural Products / 中国生物化学与分子生物学报

Nitric oxide(NO) is essential for reducing post-harvest product senescence and storage losses. NO may slow down the senescence of agricultural products and improve their quality, suggestive of its great potential in the postharvest preservation of agricultural products. The development of NO as a post-harvest preservative is supported by the investigation of its role and mechanism in agricultural product ageing. In this review, the mechanisms of NO in the post-harvest senescence of agricultural products were explored. NO is produced mainly via the nitrate reductase(NR) and NO synthase-like(NOS) pathways. Usually, NO donors include sodium nitroprusside (SNP), S-nitroso-N-acetyl-DL-penicillamine (SNAP), S-nitrosoglutathione(GSNO) and NO gas fumigation. NO delays agricultural product senescence by regulating ROS metabolism, enhancing antioxidant activity, reducing respiration, activating cell wall metabolism, inhibiting ethylene synthesis, and promoting energy metabolism. Melatonin(MT), ethylene(ETH), hydrogen gas(H

Individualized red-cell transfusion strategy for non-cardiac surgery in adults: a randomized controlled trial / 中华医学杂志(英文版)

BACKGROUND@#Red-cell transfusion is critical for surgery during the peri-operative period; however, the transfusion threshold remains controversial mainly owing to the diversity among patients. The patient's medical status should be evaluated before making a transfusion decision. Herein, we developed an individualized transfusion strategy using the West-China-Liu's Score based on the physiology of oxygen delivery/consumption balance and designed an open-label, multicenter, randomized clinical trial to verify whether it reduced red cell requirement as compared with that associated with restrictive and liberal strategies safely and effectively, providing valid evidence for peri-operative transfusion.@*METHODS@#Patients aged >14 years undergoing elective non-cardiac surgery with estimated blood loss > 1000 mL or 20% blood volume and hemoglobin concentration <10 g/dL were randomly assigned to an individualized strategy, a restrictive strategy following China's guideline or a liberal strategy with a transfusion threshold of hemoglobin concentration <9.5 g/dL. We evaluated two primary outcomes: the proportion of patients who received red blood cells (superiority test) and a composite of in-hospital complications and all-cause mortality by day 30 (non-inferiority test).@*RESULTS@#We enrolled 1182 patients: 379, 419, and 384 received individualized, restrictive, and liberal strategies, respectively. Approximately 30.6% (116/379) of patients in the individualized strategy received a red-cell transfusion, less than 62.5% (262/419) in the restrictive strategy (absolute risk difference, 31.92%; 97.5% confidence interval [CI]: 24.42-39.42%; odds ratio, 3.78%; 97.5% CI: 2.70-5.30%; P <0.001), and 89.8% (345/384) in the liberal strategy (absolute risk difference, 59.24%; 97.5% CI: 52.91-65.57%; odds ratio, 20.06; 97.5% CI: 12.74-31.57; P <0.001). No statistically significant differences were found in the composite of in-hospital complications and mortality by day 30 among the three strategies.@*CONCLUSION@#The individualized red-cell transfusion strategy using the West-China-Liu's Score reduced red-cell transfusion without increasing in-hospital complications and mortality by day 30 when compared with restrictive and liberal strategies in elective non-cardiac surgeries.@*TRIAL REGISTRATION@#ClinicalTrials.gov, NCT01597232.

Influence of ADOPT mode nursing intervention on airway self-care ability in patients after total laryngectomy / 中国实用护理杂志

Objective:To explore the effect of ADOPT mode nursing intervention on airway self-care ability in patients with total laryngectomy.Methods:50 patients who received total laryngectomy were randomly divided into control group (25 cases) and observation group (25 cases). The control group received routine nursing, while the observation group received ADOPT mode nursing intervention on the basis of routine nursing. The Exercise of Self-Care Agency Scale and self-made airway self-care knowledge questionnaire were used to evaluate the airway self-care ability and the incidence of airway related complications was also evaluated.Results:On discharge and 3 months after discharge, total scores about self-care knowledge of airway were 83.80 ± 5.06 and 89.40 ± 4.86 in the experimental group, which were significantly higher than those in the control group (68.75 ± 5.57, 72.50 ± 6.76), the differences were statistically significant ( t = -9.91, -10.09, both P<0.05). On discharge and 3 months after discharge, total scores in ESCA were 126.88 ± 9.77 and 133.60 ± 8.10 in the experimental group, which were significantly higher than those in the control group (113.29 ± 17.06 and 119.13 ± 15.30). The differences were significant ( t = -3.42, -4.12, both P<0.05). The incidence of airway complications was 41.67% (10/24) in the control group and 12.00%(3/25) in the observation group, which was statistically significant ( χ2 = 5.53, P<0.05). Conclusions:ADOPT mode nursing intervention can significantly improve the airway self-care ability of patients with total laryngectomy, reduce the incidence of airway complications, and is beneficial to patients′ physical and mental recovery.

Evaluation of CLIA plus NAT for detecting HBsAg single-ELISA-reactive samples of blood donors / 中国输血杂志

【Objective】 To study the detection performance of HBsAg single-ELISA-reactive samples of blood donors. 【Methods】 Two kinds of ELISA reagents from different manufacturers (named as reagents A and B) were used for HBsAg screening. A total of 276 samples, from January 2017 to May 2021, with HBsAg single-ELISA-reactive results were collected for further nucleic acid detection technology (NAT) and chemiluminescence immunoassay (CLIA) testing, to undergo HBV-DNA and five hepatitis B tests, respectively. The relationship between HBsAg single-ELISA-reactivity, NAT and CLIA was statistically analyzed. 【Results】 Among the 276 HBsAg single-ELISA-reactive samples, 14 were NAT reactive, with the positive rate of 5.07% (14/276). Fisher′s exact test was used to compare the compliance of reagents A and B with NAT reactivity, and the difference was not statistically significant (P<0.05). Among 14 HBsAg+ /NAT+ samples retested by CLIA, 2 were HBsAg reactive(14.29%, 2/14), 13 were anti-HBc reactive (92.86%, 13/14), 9 had the quantitative value of anti-HBs <10 mIU/mL, 5 had the quantitative value of anti-HBs between 10 to 100mIU/ mL. A total of 5 serological patterns were detected, and anti-HBe+ /anti-HBc+ pattern was the dominant. There were 262 cases of HBsAg+ /NAT- samples, but only 1 (0.38%, , 1/262) case was HBsAg reactive by CLIA, 100 were anti-HBc reactive (38.17%, 100/262), 144 (54.96%, 144/262) were anti-HBs reactive, and 1 was HBeAg reactive. A total of 8 serological patterns were detected. 【Conclusion】 Most of HBsAg single-ELISA-reactive results are false, and NAT could effectively reduce the residual risk of transfusion transmitted diseases.

Risk Identification and Evaluation of Clinical Trials of I nnovative Drugs under New Policy Environment / 中国药房

OBJECTIVE：To identify and evaluate the risk factors of innovative drug clinical trials ，and to provide references for the development of risk management and control strategies. METHODS ：Using the method of literature research ，the risk factors of innovative drug clinical trials were obtained ，classified and sorted out ，and the risk evaluation indicators were initially formed. On this basis ，the questionnaire was designed. By means of interview and questionnaire survey ，54 experts were invited from 4 tertiary hospitals and 1 contract research organization to score. SPSS 24.0 software was used to perform principal component analysis on risk event status data. RESULTS & CONCLUSIONS ：The risk evaluation system included 9 first-class indicators such as policy and legal risk ，clinical trial institution risk and trial scheme design risk ，and 31 second-class indicators such as industrial policy，law and regulation changes ，intellectual property risk . According to the analysis ，the risk factors of innovative drug clinical trials mainly included drug and biological sample management risk ，trial scheme design risk ，clinical trial institution risk ，safety report management risk ，ethical review risk ，policy and legal risk ，and subject management risk. Relevant parties in clinical trials need to focus on monitoring various risk factors ，establish dynamic monitoring and control mechanism and implementation guarantee mechanism ，and effectively prevent and control risk ，to promote the smooth implementation of clinical trials.

Knowledge, attitude and behavior of AIDS in college students in Songjiang District of Shanghai / 上海预防医学

Objective To determine the status of HIV/AIDS-related knowledge, attitude and behavior in college students, and to provide the theoretical basis for strategy of control and prevention. Methods By using random cluster sampling method, we conducted an anonymous online questionnaire survey on a total of 917 young students in Songjiang District of Shanghai. Results The awareness rate of HIV/AIDS-related knowledge was 71.8% among 903 responders. 11.1% of the students had sexual experience, and the rate was higher in male students than in female students(χ2 = 10.549, P < 0.01). The average age of first sexual intercourse was 18.4±1.3 years old, and 60.0% of the students used condom when having sex for the first time. In this survey, only 28.2% were willing to take anonymous HIV antibody urine test, and 1.8% of the students had been tested for HIV. Conclusion The overall awareness rate of knowledge of prevention and control of HIV/AIDS, the rate of condom use, and the willingness to take anonymous HIV antibody urine test are low among young college students in Songjiang District of Shanghai. We suggest to carry out targeted health education activities and to expand coverage of HIV testing by promoting the anonymous HIV antibody urine test for control the spread of HIV/AIDS among young students.

Clinical features related to lymphatic metastasis in grade 3 endometroid endometrial cancer: a retrospective cross-sectional study / 中华医学杂志(英文版)

BACKGROUND@#Endometrial cancer (EC) has been one of the most general cancers with respect to gynecological malignancies; however, there are debates on clinical strategies concerning treatments especially for patients with grade 3 (G3) endometroid endometrial cancer (EEC). Present study aimed to evaluate the lymphatic metastasis (LM) related factors and figure out the necessity of lymphadenectomy for G3 EEC patients.@*METHODS@#From January 2009 to April 2019, 3751 EC patients were admitted to Obstetrics and Gynecology Hospital of Fudan University. Clinical characteristics include age, grade, stage, and clinical pathological features. A total of 1235 EEC patients were involved in the multivariable analysis. Three hundred and eighty-one patients were involved in the survival analysis and the data attributed to sufficient follow-up information. Kaplan-Meier curve and log-rank test were utilized to analyze the survival rate.@*RESULTS@#Among the 1235 EEC patients, 181 (14.7%) were categorized as G3 and 1054 (85.3%) were grade 1 to grade 2 (G1-2). Multivariate analysis demonstrated that lymphovascular space invasion, adnexal involvement, and cervical stroma involvement were independent risk factors of LM in G3 cohort with odds ratio 3.4, 5.8, and 8.9; 95% confidence interval 1.1-10.6, 1.5-22.4, and 2.8-28.0, respectively. LM rates increased from 3.3% (3/92) to 75% (9/12) for G3 EEC cohort as related factor numbers increased from one to three. There were no differences between G3 and G1-2 EEC in overall survival and progression free survival. Additionally, no survival advantage was observed for G3 EEC patients at early stage with different plans of adjuvant treatment.@*CONCLUSIONS@#For G3 EEC patients without other pathological positive factor, the LM rate is lower than those with other pathological positive factor. Survival analysis showed no difference between G3 cohort and G1-2 cohort. Also, different adjuvant treatments had no impact on the overall survival for G3 EEC patients.

Profile of irregular antibodies among voluntary blood donors: 2018 to 2020 in Jiaxing / 中国输血杂志

【Objective】 To study and analyze the profile of irregular antibodies among voluntary blood donors in Jiaxing area. 【Methods】 The ABO and RhD blood groups of all voluntary blood donors from November 2018 to November 2020 were detected by DIAGAST QWALYS 3 automatic blood group analyzer. According to routine serological screening program of irregular antibody, the samples reactive to O blood cell were sent to the reference laboratory for further identification of the antibody specificity, and the specificity and distribution characteristics of irregular antibodies were analyzed statistically. 【Results】 A total of 79 samples presenting irregular antibodies were yielded out of 90 854 blood samples, with a positive rate of 0.087%. More female samples (n=44) than male (n=35) (P0.05). 【Conclusion】 Female, RhD negative and more than 45 years old blood donors are more likely to present irregular antibodies, regardless of the number of blood donations.

Evaluation of continuous metabolic syndrome score as a screening tool for metabolic syndrome in Xinjiang urban population aged 15-18 years / 中国学校卫生

Objective@#The purpose of the present study was to evaluate of continuous metabolic syndrome score (cMetS) in screening metabolic syndrome (MetS) and to determine the cut-off values in a representative sample of Xinjiang population aged 15 to 18 years old.@*Methods@#A stratified cluster sampling was used to select participants aged 15-18 years from 13 cities in Xinjiang. cMetS was calculated by summing up the Zscores of standardized waist circumference, mean arterial pressure, high-density lipoprotein, triglyceride, and fasting blood glucose by age and gender.@*Results@#Totally 16.3% of subjects were overweight, and 5.2% were obese. The proportion of overweight and obesity in males was significantly higher than that in females (18.1% vs 13.8%)(6.0% vs. 4.1%)(χ2=15.36，7.89，P<0.05). The prevalence of total MetS was 6.1%, with 6.6% and 5.4% for men and women, respectively. There was a correlation between cMetS and MetS components, and the average cMetS value increased with increasing MetS component(P<0.05). The total cut-off value of cMetS was 0.99 (sensitivity 68.0%, specificity 80.7%), and the area under the ROC curve was 84.9%. The cMetS scores for boys and girls were 0.80 and 1.48, respectively.@*Conclusion@#Compared with MetS, cMetS shows more accuracy in screening MetS among youth aged 15-18 years in both gender.

AIDS sentinel surveillance of female sex workers in Songjiang District of Shanghai / 上海预防医学

Objective To investigate the knowledge of HIV/AIDS among the female sex workers in Songjiang District of Shanghai, changes in their related behaviors, and prevalence of human immunodeficiency virus(HIV), syphilis, and hepatitis C virus (HCV), providing evidence for related prevention and control work in this regard. Methods During 2011-2015 sentinel surveillance period, in the area of Songjiang District, according to the survey method of the National AIDS Sentinel Monitoring Implementation Program, questionnaires were conducted on the female sex workers and their venous blood was collected to detect HIV, syphilis and HCV antibodies. Results A total of 2 064 secret prostitutes were surveyed.The overall awareness rate of AIDS-related knowledge among them was 94.77%, and the annual awareness rates were 93.33%, 95.01%, 93.00%, 99.76%, and 92.57%, respectively(P < 0.05);The total proportion of the female sex workers who used condoms in the last commercial sex was 90.07%, and 84.05%, 80.55%, 93.72%, 99.06%, and 95.79% respectively in the calendar year(P < 0.05);The total proportion of the female sex workers who insisted on using condoms for commercial sex in the last 1 month was 76.74%, and 69.29%, 60.60%, 86.47%, 87.76%, and 78.96% respectively in the calendar year(P < 0.05);The average detection rate of HIV antibodies for 5 years was 0.00%, syphilis antibody detection rate was 3.00%, and HCV antibody detection rate was 0.34%. Conclusion The knowledge level of AIDS related knowledge is high among the female sex workers in Songjiang District.The persistent condom use rate during commercial sex is low in the last month; the syphilis detection rate is slightly higher than the national average.

AIDS sentinel surveillance of female sex workers in Songjiang District of Shanghai / 上海预防医学

Objective To investigate the knowledge of HIV/AIDS among the female sex workers in Songjiang District of Shanghai, changes in their related behaviors, and prevalence of human immunodeficiency virus(HIV), syphilis, and hepatitis C virus (HCV), providing evidence for related prevention and control work in this regard. Methods During 2011-2015 sentinel surveillance period, in the area of Songjiang District, according to the survey method of the National AIDS Sentinel Monitoring Implementation Program, questionnaires were conducted on the female sex workers and their venous blood was collected to detect HIV, syphilis and HCV antibodies. Results A total of 2 064 secret prostitutes were surveyed.The overall awareness rate of AIDS-related knowledge among them was 94.77%, and the annual awareness rates were 93.33%, 95.01%, 93.00%, 99.76%, and 92.57%, respectively(P < 0.05);The total proportion of the female sex workers who used condoms in the last commercial sex was 90.07%, and 84.05%, 80.55%, 93.72%, 99.06%, and 95.79% respectively in the calendar year(P < 0.05);The total proportion of the female sex workers who insisted on using condoms for commercial sex in the last 1 month was 76.74%, and 69.29%, 60.60%, 86.47%, 87.76%, and 78.96% respectively in the calendar year(P < 0.05);The average detection rate of HIV antibodies for 5 years was 0.00%, syphilis antibody detection rate was 3.00%, and HCV antibody detection rate was 0.34%. Conclusion The knowledge level of AIDS related knowledge is high among the female sex workers in Songjiang District.The persistent condom use rate during commercial sex is low in the last month; the syphilis detection rate is slightly higher than the national average.

Research Progress of Animal Model of Tic Disorder and Its Application in Traditional Chinese Medicine / 中国实验方剂学杂志

Tic disorder （TD） is a neurodevelopmental disorder， with one or more motor and/or vocal disorders as the main symptoms. It brings many inconveniences to children's learning and life， and has a profound impact on children's character building. The pathogenesis of TD is mainly correlated with neurotransmitter release disorder， neuroimmune， genetic， trace element imbalance， diet and other factors， but has not been completely clear up to now. Western Medicine has obvious effects on TD， but with serious side effects. Compared with western medicine， traditional Chinese medicine （TCM） has the advantages of low adverse reactions and definite and lasting effect， and thus has been widely recognized by children and their families. In order to explore the pathogenesis of TD and the specific mechanism of TCM in the treatment of TD， many scholars have carried out a large number of in-depth animal experiments and made some achievements， but also exposed some defects， such as the single modeling method， failed to take into account other pathogenesis of TD， failure to combine the specific syndromes of TCM for targeted modeling， and failure to reflect the dialectic of TCM on the characteristics of governance. This paper reviews the modeling methods of common animal models， the comparison of advantages and disadvantages， and the changes of behavioral and biochemical indicators before and after the intervention with TCM compounds on TD animal models， so as to provide reference for the selection of animal models in future animal experimental research.

Relationship between the expression of microRNA-16, microRNA-124 and microRNA-195 in peripheral blood of schizophrenic patients and their cognitive and social functions / 中国基层医药

Objective:To investigate the correlation between the expression of microRNA-16, mircoRNA-124 and mircoRNA-195 in peripheral blood of schizophrenic patients and their cognitive and social functions.Methods:From January 2016 to December 2018, 112 schizophrenic patients admitted to the Second People's Hospital of Taizhou were selected as observation group, and 93 healthy examinees from the Second People's Hospital of Taizhou were selected as control group.The expression of mircoRNA-16, mircoRNA 124 and mircoRNA 195 in peripheral blood was detected by real-time quantitative fluorescent PCR.The cognitive function of patients was evaluated by Chinese version of Montreal Cognitive Assessment Scale (MoCA), and the social function of inpatients with mental illness was evaluated by SSPI.Results:The expression of mircoRNA-16 (0.03±0.01) and mircoRNA 195(0.08±0.03) in peripheral blood of the observation group was lower than that of the control group[(0.12±0.02) and (0.27±0.06)], while the expression of mircoRNA 124 (14.63±3.24) of the observation group was higher than that of the control group (7.45±1.39), the differences were statistically significant( t=41.763, 19.898, 29.389, all P<0.05). The score of MoCA scale in the observation group[(22.17±3.45)points]was lower than that in the control group[(28.39±1.28)points]( t=16.465, P<0.05). The SSPI score of the observation group[(26.58±5.16)points]was lower than that of the control group[(45.37±3.27)points]( t=30.405, P<0.05). MicroRNA-16 and microRNA-195 were positively correlated with MoCA scale score and SSPI scale score ( r=0.641, 0.724, SSPI scale score: r=0.801, 0.657, all P<0.05), while microRNA-124 was negatively correlated with MoCA scale score and SSPI scale score ( r=-0.738, -0.769, all P<0.05). Conclusion:The expression of mircoRNA-16 and mircoRNA-195 in peripheral blood of schizophrenic patients decreased, while the expression of mircoRNA-124 increased.The expressions of mircoRNA-16 and mircoRNA-195 were positively correlated with cognitive and social functions, while mircoRNA-124 was negatively correlated with cognitive and social functions.

Mechanism of BAZ1A regulating radiosensitivity of lung cancer cells / 西安交通大学学报(医学版)

Objective: To investigate the effect of siRNA silencing BAZ1A on radiosensitization of human lung adenocarcinoma A549 cells. Methods :A549 cells were randomly divided into transfection reagent control (Ctrl) group, negative control siRNA (siNC) group, and siBAZ1A group. The expression of BAZ1A protein was evaluated by Western blot. The clone formation assay was applied to investigate the survival fraction (SF) of the A549 cells treated with different radiation doses (0, 2, 4, 6, 8, and 10Gy), and one-hit multi-target model was applied to analyze the radiation dose survival curve. MTS assay, scratch assay, and flow cytometry were utilized to determine the relative survival, cell migration abilities, and apoptosis, respectively. Results: The expression level of BAZ1A protein in A549 cells was inhibited by BAZ1A-siRNA transfection. X-ray radiation inhibited the colony formation capacity of A549 cells, and the SF of siBAZ1A group was lower than that of the other two groups with radiation (P<0.05). Compared with those of Ctrl group, the sensitization enhancement ratio (SER) of siNC and siBAZ1A groups was 1.06 and 2.24. Moreover, with the transfection of BAZ1A-siRNA, the relative survival rate and cell migration ability were decreased after X-ray radiation. Besides, the apoptosis rate was higher in siBAZ1A group (P<0.05). Conclusion: Silencing the expression of BAZ1A by siRNA can efficiently improve the sensitization of radiotherapy for A549 cells.

Anti-inflammatory mechanism of Xixian Tongshuan Preparation based on molecular simulation methods / 中国中药杂志

Inflammatory response is caused by exogenous and endogenous stimuli,resulting in a non-specific resistance reaction.After acute ischemic cerebral infarction,inflammatory factors gather and adhere in the ischemic area of leukocyte infiltration,and the released inflammatory factors causes the injury cascade,aggravate the brain tissue damage and the symptoms of neurological deficits,and hinder the repair of brain neurons and the recovery of nerve function. In this paper,the key targets in the arachidonic acid metabolic pathway were studied. The Hiphop pharmacophore model of s PLA2-ⅡA and COX-2 inhibitors was built. According tothe two previously constructed 5-LOX and LTA4 H target inhibitors,the pharmacophore model was used to initially screen out the composition database of all of 13 traditional Chinese medicines in Xixian Tongshuan Preparation. The molecular matching study was carried out by selecting the matching value greater than 0. 6,and the component with the CDOCKER score greater than 80% of the original ligand score was used as the potential active inhibitor of the target. Considering the pharmacophore matching value,the molecular docking score and the interaction between the components and the target,one Chuanxiong component and one safflower component were selected as potential inhibitors of s PLA2-ⅡA; two Chuanxiong components,two Panax notoginseng,one safflower component,one angelica component,one valerian component were taken as a potential inhibitor of COX-2; two Gentiana components,one safflower component,one valerian component,one P. notoginseng component and one Angelica component were taken as potential inhibitors of 5-LOX; and two Gentiana components,two Chuanxiong components,and two safflower components were taken as potential inhibitors of LTA4 H. This study screened out the potential inhibitors of the four targets in a high-efficiency and low-cost manner,and explained that Xixian Tongshuan Preparation showed an effect in the treatment of inflammatory responses caused by ischemic stroke by acting both LOX pathway and COX pathway in the metabolic pathway.

Anti-platelet aggregation mechanism of Xixian Tongshuan Preparation based on molecular simulation methods / 中国中药杂志

The thrombus is a deposit that is formed on the surface of the endovascular or at the site of repair,and known as the main complication of cardiovascular disease and the cause of death. At the same time,thrombus is mainly treated by the following three ways: anticoagulation,anti-platelet aggregation and thrombolysis. In this study,the chemical constituents of seven traditional Chinese medicines in the Xixian Tongshuan Preparation were collected to construct a component database. Subsequently,the pharmacophore were used to screen out the component database,and molecular docking was used to screen out the results of pharmacophore for explaining the material basis and mechanism that Xixian Tongshuan Preparation exerts anti-thrombotic activity by inhibiting platelet aggregation. First of all,P2 Y12,GPⅡb/Ⅲa and PAR1 were selected as study vectors,the optimal models of inhibitors were obtained respectively through verification and evaluation of the pharmacophore models. Afterwards,the component database was screened out by the optimal pharmacophore models of PAR1,P2 Y12 and GP Ⅱ b/Ⅲ a,and the molecular docking method was used to further refine the screening results. The screening results indicated that the anti-platelet aggregation effect of Xixian Tongshuan Preparation was correlated with the inhibition of P2 Y12,PAR1 and GPⅡb/Ⅲa expressions with saffower yellower,hirudin and candidin and notoginseng triterpenes,folinic acid,respectively. The material basis and mechanism of anti-platelet aggregation of Xixian Tongshuan Preparation provided a theoretical basis for the clinical use of the preparation and the lead compounds for the development of anti-platelet aggregation drugs.

Multi-target neuroprotective effect of Xixian Tongshuan Preparation against cerebral ischemia injury / 中国中药杂志

In this paper, Xixian Tongshuan Preparation was used as the research object, and all the chemical components of the 13 traditional Chinese medicines were collected. The target finding technique was used to obtain the key targets of the neuroprotective effect of Xixian Tongshuan Preparation, including 5 glutamate receptors, TGFR-1 and VEGFR-2. Molecular docking technology was used to screen out the potential active components of the above targets and to analyze their mechanism of action. It was found that single component, such as neo-complanatoside and neo-carthamin, in Xixian Tongshuan Preparation could simultaneously act on different targets. The chemical constituents in Ligusticum chuanxiong, Angelica sinensis, Carthamus tinctorius, and Panax pseudo-ginseng could simultaneously act on different neuroprotective-related targets, which reflected the application of multi-components to multi-targets. Point and multiple sites played a key role in protecting neurons against cerebral ischemic injury. This study explains the multi-target mechanism of anti-cerebral ischemic injury in neuroprotection at the molecular level, and provides a certain direction for the clinical application and experimental research of Xixian Tongshuan Preparation.

Lipid regulation mechanism and dosage form selection of Xixian Tongshuan Preparation based on molecular simulation methods / 中国中药杂志

Atherosclerosis is the main cause of stroke, and dyslipidemia is the most important risk factor for atherosclerosis. In this paper, pharmacophore and molecular docking models of eight key lipid-lowering targets, namely NPC1 L1, HMG-CoA reductase, SQS, MTP, CETP, PPARα, LXRα and LXRβ, were used to screen out the small molecular database of traditional Chinese medicine(TCM), which was made up of ingredients of thirteen Chinese herbal medicines contained in Xixian Tongshuan Preparation. The screening results indicated that the preparation could showed an effect in regulating lipid on target NPC1 L1, HMG-CoA reductase, LXRβ and SQS through four groups of potential active compounds, namely prupersin A in peach kernel and suffruticoside A in gastrodiaelata, limocitrin-β-D-glucoside in Ligusticum chuanxiong, 2'-(2,3-dihydroxybenzoyl)-sweroside in Pinellia ternate and quercitrin in Panax notoginseng, 4-tert-butyl-2-[(5-tert-butyl-2-hydroxy-phenyl)methoxy-methyl]-6-(hydroxymethyl)phenol in Gastrodia elata. Moreover, the properties and extraction process of the most potentialactive compounds were consistent with the preparation process of Xixian Tongshuan Capsules, which indicated that the capsule had more advantages than the pill in the existing two dosage forms of Xixian Tongshuan Preparation. This study analyzed the pharmacodynamic basis and mechanism of Xixian Tongshuan Capsules in regulating lipid for treating stroke, and provided evidence for its further research and clinical application.

Fertility-preserving treatment for early stage endometrial cancer / 中国实用妇科与产科杂志

The fertility preservation treatment has become one of the main issues with the increasing incidence of endometrial cancer.Strict selection of the young endometrial cancer patients who are eligible for fertility preservation treatment and individualizedtreatment plan are fundamental for improving treatmenteffect while decreasing the risk of treatment-relatedcomplications and treatment failure.This article willdiscuss the evaluation and treatment choice for fertilitypreservation in young endometrial cancer patients.