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Objective:To establish liver fibrosis models in cynomolgus monkey using calf serum.Methods:Healthy cynomolgus monkeys were randomly divided into control group and model group,and there were 6 animals in each group.Model group were treated with calf serum subcutaneously at the dose of 0.4 ml/kg,and control group were received the same dose of normal saline.Calf serum will be administrated twice a week.Results:After 8 weeks of administration,the serum HA,PC Ⅲ,Ⅳ C,TGF-β1 and α-SMA in model group were increased differently when compared with modeling before(P＜0.05,P＜0.01),and the serum HA and PC Ⅲ in model group were also increased significantly when compared with control group.After 12 weeks of administration,the serum Ⅳ C,TGF-β1 and α-SMA in model group were increased significantly compared with control group(P＜0.05),and the serum LN and TCH in model group were increased obviously when compared with modeling before(P＜0.05),the levels of serum AST and ALT in model group were also increased distinctly as compared with control group and modeling before(P＜0.05),while the serum ALB in model group was decreased significantly when compared with modeling before(P＜0.05).At week 16,the serum LN,TCH and TG in model group were increased significantly when compared with control group(P＜0.05);while the serum ALB in model group was decreased distinctly when compared with control group(P＜0.05);the strong echo were showed in liver of model group by using ultrasonography;pathological examination showed that there were necrosis and monocyte infiltrating in liver of model group,and collagen fiber proliferation were observed in portal area of model group.Conclusion:Liver fibrosis models can be established successfully in cynomolgus monkeys by subcutaneously injecting calf serum for 16 weeks.
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Objective To develop a portable device for wound perfusion and negative-pressure therapy to solve the problem of the existing one.Methods The device used a unified waste liquid tank/liquid reservoir as the infrastructure,and had its reservoir and waste liquid bags made of flexible materials.Dynamic isolation between the reservoir bag and waste liquid bag was realized by a separator.Wound perfusion was implemented by the gravity of the waste liquid and the elastic force of the spring, and negative-pressure therapy was carried out by the elastic force and vacuum pump. The SCM regulated the switching of the electromagnetic valve according to the information from the flow meter and pressure sensor so as to perform quantitative wound perfusion, stable negative-pressure therapy and switching between the functions of perfusion and negative-pressure therapy.Results The device volume was decreased by regulating dynamically the sizes of reservoir and waste liquid bags,and the power consumption was also reduced by using liquid gravity and spring force. Conclusion The wound perfusion and negative-pressure therapy device gains advantage over the existing one in portability, and thus is of significance for promoting the technology of wound perfusion and negative-pressure therapy.