Chemical study on ethyl acetate portion of Ehretia thyrsiflora, boraginaceae species of Kudingcha / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of Ehretia thyrsiflora.</p><p><b>METHOD</b>Compounds were isolated by using silica gel, Sephadex LH-20 and RP-C18 chromatography; their structures were elucidated by means of spectral data analysis.</p><p><b>RESULT</b>Seven compounds were isolated and identified as methyl rosmarinate (1), caffeic acid (2), quercetin (3), kampferol (4), kaempferol 3-O-alpha-D-arabinoside (5), quercetin 3-O-alpha-D-arabinoside (6), and p-hydroxy benzoic acid (7).</p><p><b>CONCLUSION</b>All these compounds were isolated from E. thyrsiflora for the first time. Compounds 2-7 were isolated from genus Ehretia for the first time.</p>

Determination of two polyacetylenes in Herba Lobeliae Chinensis by HPLC analysis / 中国中药杂志

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.</p><p><b>METHOD</b>C18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.</p><p><b>RESULT</b>Lower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.</p><p><b>CONCLUSION</b>A simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.</p>