Xiangsha Liu Junzitang for Prevention and Treatment of Gastric Cancer： A Review / 中国实验方剂学杂志

Gastric cancer is one of the malignancies with high incidence in the world. Xiangsha Liu Junzitang，a common prescription for the prevention and treatment of gastric cancer，has the effects of moving Qi to relieve pain，drying dampness， and invigorating the spleen. It is especially indicated for gastric cancer of the spleen and stomach qi deficiency syndrome. Based on the databases such as CNKI，Wanfang Data，and PubMed，the clinical efficacy and experimental studies of Xiangsha Liu Junzitang for the prevention and treatment of gastric cancer were summarized and sorted out，and the mechanism of Xiangsha Liu Junzitang for the prevention and treatment of gastric cancer was elaborated in order to provide useful references for the clinical and basic research on Xiangsha Liu Junzitang in the field of gastric cancer in the future. In clinical practice，Xiangsha Liu Junzitang can treat gastric precancerous lesions，increase the body immunity of patients with gastric cancer，improve the symptoms of spleen and stomach weakness after gastric cancer surgery，and reduce the adverse reactions of the digestive tract after chemotherapy for gastric cancer. Its clinical efficacy is superior to that of western medicine alone whether it is combined with western medicine or used alone. In the experimental research，Xiangsha Liu Junzitang has the effects of regulating inflammatory factors，inhibiting the proliferation of gastric cancer cells，promoting the apoptosis of gastric cancer cells，and improving the activity of pepsin. Modern pharmacological research has shown that Xiangsha Liu Junzitang can conduct a comprehensive intervention with multiple components and multiple targets. The main components of a single drug contained include saponins，polysaccharides，lactones，volatile oils，organic acids，and others， with the effects of protecting gastric mucosa，regulating endocrine，and promoting apoptosis of epithelial cells in gastric mucosal dysplasia，reflecting the advantages and values of traditional Chinese medicine in the prevention and treatment of gastric cancer.

Placebo control and its methodological issues on clinical trials of acupuncture therapy / 中国针灸

The paper introduces the placebo acupuncture simulation devices commonly used in clinical trial of acupuncture therapy. These devices are composed of Streitberger, Park, Takakura, Foam and Phantom acupuncture. Because acupuncture therapy is a kind of complex intervention, there are the controversies in methodology for the acupuncture placebo control of clinical trial. Placebo acupuncture may be an effective control, with a certain of specific therapeutic effect. The blinding effect of placebo acupuncture is highly questioned, specially, the sensation of deqi is hardly imitated during acupuncture. On these grounds, in this research, the suggestions has been proposed on the selection and the setting of placebo control in clinical trial of acupuncture therapy.

Herbal Textual Research on Scrophulariae Radix in Famous Classical Formulas / 中国实验方剂学杂志

In this paper， the name， origin， producing area， quality evaluation， harvesting and processing methods of Scrophulariae Radix used in the famous classical formulas were researched by consulting related ancient materia medica， medical books and prescription books. The results showed that the name of Scrophulariae Radix originated from its shape and color. Scrophularia ningpoensis， one of the eight flavors of Zhejiang， has been highly praised in the past as a genuine base， and its authentic production area is Zhejiang. Quality of Scrophulariae Radix is best if it has a dry body， thin skin， thick branches， firm physique， black inner color， net basal part of stem， and no fine whiskers. In ancient times， the origin processing was divided into two types， including sun-dried after steaming and directly sun-dried. While it mostly used a combination of drying and sweating in modern times. Before the Ming dynasty， the processing of Scrophulariae Radix was mainly steamed. Occasionally， the stir-frying appeared. In the Ming dynasty and after the Ming dynasty， wine processing emerged such as hot wine mixed roasting， wine washing， wine steaming， because the ancients believed that wine could enhance its efficacy and reduce its cold property. In the Qing dynasty， the method of steaming and baking appeared in the water and fire system. According to the 2020 edition of Chinese Pharmacopoeia， the processing of Scrophulariae Radix includes removing rhizome residues and impurities， washing， moistening， cutting into thin slices， and drying， or soaking slightly， steaming thoroughly， drying slightly， cutting into thin slices， and drying. Referring to the relevant national documents， it is suggested that raw products of Scrophulariae Radix should be used in Liangditang and Simiao Yong′antang.

Herbal Textual Research on Maltose in Famous Classical Formulas / 中国实验方剂学杂志

In this paper， the name， varieties， raw materials and manufacturing technology of maltose in the famous classical formulas were researched by consulting the herbal medicines， medical books， prescription books and modern literature of past dynasties， which provided the basis for the development and utilization of formulas containing maltose. Through textual research， it can be seen that the name of maltose has been derived from its shape， texture， preparation method， raw materials and producing area. In ancient times， maltose was mainly divided into soft and hard types according to the texture. Those who are wet and soft as honey are called "syrup" or "jelly"， while those who are hard and white are called "malt" and "sugar". In modern times， they are mostly called malt sugar， only jelly is used as medicine， and malt is mostly used as food. Throughout the ages， medicinal maltose were made of Oryza sativa var. glutinosa as the raw material and Hordeum vulgare as malt， prepared by fermenting， decocting and concentrating. The maltose made from other cereals such as Setaria italica var. germanica， Panicum miliaceum is slightly inferior in quality. The 2020 edition of Chinese Pharmacopoeia did not include maltose， but included malt sugar， a pharmaceutical excipient， which was a crystal powder with high purity. But maltose was included in the national food standard and the local processing specification. Based on the textual research results， it is suggested that malt syrup in GB/T 20883-2017 can be used as the reference for the development of formulas containing maltose， and O. sativa var. glutinosa， H. vulgare are clearly used as raw materials.

Study on Mechanism of Quercetin in Heart Failure Based on Network Pharmacology and Experimental Validation / 中国实验方剂学杂志

Objective:To predict the therapeutic targets and related signaling pathways of quercetin in the treatment of heart failure （HF） by network pharmacology and molecular docking methods，and further clarify its mechanisms through <italic>in vitro</italic> cell model. Method:The pharmacological targets of quercetin were obtained by SwissTargetPrediction and Targetnet databases； the heart failure related targets were obtained by Online Mendelian Inheritance in Man（OMIM），GeneCards and Therapeutic Target Database（TTD） databases； the protein-protein interaction（PPI） network was analyzed by STRING database（Search Tool for Recurring Instances of Neighbouring Genes），and the PPI network diagram of quercetin for heart failure target was established. Cytoscape 3.7.2 software was used for analyzing and screening the anti-heart failure network nodes of quercetin，and the obtained targets were enriched with gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） analysis by DAVID database. In order to explore the mechanism of quercetin in the treatment of heart failure，we used cell model to verify the function in heart failure treatment. Results:The predicted results showed that there were 23 targets for the treatment of heart failure，such as Matrix Metallopeptidase-9（MMP-9），androgen receptor（AR），coagulation factor 2（F2），insulin like growth factor 1 receptor（IGF1R），epidermal growth factor receptor（EGFR），janus kinase-2（JAK2），cytochrome P450 family 19 subfamily A member 1（CYP19A1），estrogen receptor-1（ESR1），tumor necrosis factor（TNF），protein tyrosine phosphatase receptor type C（PTPRC） and cytochrome P450 family 17 subfamily A member 1（CYP17A1） etc. The results suggest that quercetin may play a role in the treatment of heart failure by intervening in the physiological processes of cardiovascular cell proliferation and metabolism，regulating hypoxia-inducible factor 1 （HIF-1）signaling pathway and steroid hormone biosynthesis. Conclusion:Quercetin has the characteristics of multi-target，multi-channel and multi-channel in the treatment of heart failure. It may play a role in the treatment of heart failure by regulating MMP-9，EGFR and other key genes，participating in the biological process of cardiac and vascular cell proliferation and metabolism.

Effects of exposure to low-dose ionizing radiation on changing platelets: a prospective cohort study

BACKGROUND@#Numerous studies have concentrated on high-dose radiation exposed accidentally or through therapy, and few involve low-dose occupational exposure, to investigate the correlation between low-dose ionizing radiation and changing hematological parameters among medical workers.@*METHODS@#Using a prospective cohort study design, we collected health examination reports and personal dose monitoring data from medical workers and used Poisson regression and restricted cubic spline models to assess the correlation between changing hematological parameters and cumulative radiation dose and determine the dose-response relationship.@*RESULTS@#We observed that changing platelet of 1265 medical workers followed up was statistically different among the cumulative dose groups (P = 0.010). Although the linear trend tested was not statistically significant (P@*CONCLUSION@#We concluded that although the exposure dose was below the limit, medical workers exposed to low-dose ionizing radiation for a short period of time might have increased first and then decreased platelets, and there was a dose-response relationship between the cumulative radiation dose and platelets changing.

Advances in research on chemical composition of Picrorhiza scrophulariiflora and P. kurroa and their biological activities / 中国中药杂志

At present, 141 compounds have been isolated from Picrorhiza scrophulariiflora and P. kurroa of the Scrophulariaceae plants, including 46 iridoid glycosides, 29 tetracyclic triterpenoids, 25 phenylpropanoids, and 11 phenylethanoid glycosides. Pharmacological studies have demonstrated that they have liver-, heart-, brain-, kidney-, and nerve cells-protecting effects as well as anti-tumor, anti-inflammatory, anti-bacterial, anti-asthma, anti-diabetic, immunomodulatory, and blood lipid-lowering activities. This article reviews the chemical components and pharmacological activities of P. scrophulariiflora and P. kurroa, aiming to provide a basis for the in-depth research, development, and utilization of the two plants.

Simultaneous determination of 14 chlorinated hydrocarbons in urine by headspace gas chromatography-mass spectrometry / 中国职业医学

OBJECTIVE: To establish a headspace gas chromatography-mass spectrometry method for the determination of 14 chlorinated hydrocarbons in urine. METHODS: The urine sample 4.00 mL and anhydrous sodium sulfate 3.00 g were added into a 10.00 mL headspace bottle, then the headspace bottle was maintained at 70 ℃ for 40.0 min. After headspace pretreatment, 14 chlorinated hydrocarbons in headspace air were separated in the DB-5 MS capillary column of the gas chromatography and detected by mass spectrometer. RESULTS: There was a good linear relationship of 14 chlorinated hydrocarbons in urine in the range of 0.62-1 630.00 μg/L. The linear correlation coefficient was greater than 0.999 0.The minimum detectable concentration was 0.19-0.43 μg/L and the minimum quantitative concentration was 0.62-1.44 μg/L. The average recovery rate was 89.8%-107.1%. The within-run relative standard deviation(RSD) was 4.0%-8.5% and the between-run RSD was 6.3%-9.1%. Urine samples can be stored at 4 ℃ or-8 ℃ for 3 days and below-20 ℃ for 7 days. CONCLUSION: This method is rapid, simple, sensitive, accurate and has little interference,which can be used as a method for detecting 14 kinds of chlorinated hydrocarbons in urine samples of patients with occupational poisoning.

Protective effect of the Diaphragma Juglandis Fructus extracts on the rhabdomyolysis-induced acute kidney injury in rats / 国际药学研究杂志

Objective: To investigate the protective effect and possible mechanism of Diaphragma Juglandis Fructus(DJF) extracts on the rhabdomyolysis-induced acute kidney injury(AKI)in Sprague-Dawley(SD)rats. Methods: Sixty male SD rats were randomly divided into 6 groups: normal control group, model group as well as the four test groups treated with 180 mg/kg and 360 mg/ kg water extract or alcohol extract of DJF. The rats were orally administered once a day by gavage with the designed concentration of test substances or distilled water(for controls)for 30 days. After the last drug administration, all rats except for those in the normal control group were subjected to the intramuscular injection of 50% glycerol to both hind limbs to create the rat model of rhabdomyolysis-induced AKI. The renal function and kidney histopathology were then examined and the serum level of tumor necrosis factor-alpha (TNF-ɑ)and antioxidant indicators were detected at 24 and 48 hours after the glycerol injection. Results: Compared with the normal control group, the urine volume for 24 h as well as the serum glutathione peroxidase(GSH-Px)and total superoxide dismutase(TSOD)activities were significantly decreased, while the serum creatinine(Cr), blood urea nitrogen(BUN), TNF-ɑ and malondialdehyde(MDA)level was significantly increased in the model group(P0.05). However, the intervention with the 360 mg/kg DJF water extract significantly increased the urine volume for 24 h, enhanced the serum T-SOD activity on the 48 h, and decreased the serum Cr, BUN, MDA and TNF-α level on the 48 h(P<0.05 or P<0.01). Conclusions: Both the 360 mg/kg DJF alcohol and water extracts could effectively protect the rhabdomyolysis-induced AKI, which caused by glycerol in SD rats, and the effect of the alcohol extract was better than that of the water extract. The possible mechanism is likely related to the inhibition of inflammation and a positive role in antioxidation.

2018 China guideline for diagnosis and treatment of senile osteoporosis / 中国实用内科杂志

According to the procedures for the development of evidence-based medicine guidelines, a multi-disciplinary guideline development working group was established, after three rounds of discussions by the consensus expert group, a new evidencebased guideline for diagnosis and treatment of senile osteoporosis in China(2018) was developed. The grading of recommendations assessment, development and evaluation(GRADE) system was used to rate the quality of evidence and the strength of recommendations. Recommendations were derived from evidence body, and at the same time considered the balance of benefits and harms as well as values and preferences of Chinese patients. The guideline development working group developed 15 recommendations for the diagnosis and treatment of senile osteoporosis. The guideline covered the screening for senile osteoporosis, risk assessment, diagnosis, basic treatment, multiple anti-osteoporosis drugs, therapeutic effect monitoring and evaluation of senile osteoporosis. This guideline aims to serve as a tool for clinicians and patients for best decisions-making in China.

Clinical effect of double filtration plasmapheresis combined with glucocorticoid and immunosuppressant in treatment of children with severe Henoch-Schönlein purpura nephritis / 中国当代儿科杂志

OBJECTIVE@#To study the clinical effect and safety of double filtration plasmapheresis (DFPP) combined with double pulse therapy with methylprednisolone (MP) and cyclophosphamide (CTX) in the treatment of children with severe Henoch-Schönlein purpura nephritis (HSPN).@*METHODS@#A total of 60 children with severe HSPN who were admitted to the hospital from January 2014 to March 2018 were enrolled and were randomly divided into an observation group and a control group (n=30 each). In addition to routine treatment, the children in the control group were given MP+CTX pulse therapy. Those in the observation group were given DFPP treatment in addition to the treatment in the control group, with three courses of treatment in total. After three courses of treatment, the two groups were compared in terms of 24-hour urinary protein, urinary microproteins, renal function parameters, adverse reactions, and clinical outcome.@*RESULTS@#After three courses of treatment, the observation group had significantly greater reductions in 24-hour urinary protein, urinary albumin, urinary immunoglobulin G, urinary β2-microglobulin, serum creatinine, and blood urea nitrogen than the control group (P0.05).@*CONCLUSIONS@#Compared with MP+CTX pulse therapy alone in the treatment of severe HSPN in children, DFPP combined with MP+CTX pulse therapy can further alleviate renal injury and improve clinical outcome and does not increase the incidence rate of adverse reactions.

Effects of n-hexane on the serum level of three neurotoxicity biomarkers in rat / 中国职业医学

OBJECTIVE: To observe the effect of n-hexane subchronic exposure on the serum level of neuron specific enolase(NSE), neurofilament light chain protein(NF-L) and nerve growth factor(NGF) in rat, and to explore the feasibility of using NSE, NF-L and NGF as biomarkers of n-hexane neurotoxicity. METHODS: Specific pathogen free male SD rats were randomly divided into control group and low-, medium-and high-dose exposure groups, with 6 rats in each group. Rats in the low-, medium-and high-dose exposure groups were given n-hexane solution at doses of 168, 675 and 2 700 mg/kg body mass, respectively, while rats in the control group were gavaged with 0.9% sodium chloride solution, once a day, 5 days a week for 6 weeks. At week 0, 2, 4, and 6 of exposure, the body mass of the rats was weighed, the gait scores were performed, and the serum levels of NSE, NF-L, and NGF were detected.RESULTS: Body mass, gait score and serum levels of NSE and NF-L in rats were statistically significant in terms of the n-hexane exposure dose and exposure time(P<0.01). At the 6 th week of n-hexane exposure, the body mass of the three dose exposure groups was lower than that of the control group(P<0.05), and the gait score was higher than that of the control group(P<0.05). Moreover, the abnormal gait of the rats showed a dose-effect relationship with the increasing n-hexane poisoning dose. At week 2, 4 and 6, the serum levels of NSE and NF-L in these three dose exposure groups were higher than that in the control group(P<0.05). In addition, the serum level of NF-L in rats in the medium-and high-dose exposure groups increased with the n-hexane exposure time increasing and showed a time-effect relationship(P<0.05). The level of serum NGF in rats was statistically significant in the main effects of n-hexane dose and duration of exposure(P<0.05). The serum NGF level in the high-dose exposure group was lower than that in the control group, the low-dose and medium-dose exposure groups(P<0.05). NGF level in serum of rats at week 6 was lower than that at week 0, 2 and 4(P<0.05). CONCLUSION: Both NSE and NF-L in serum can be used as biomarkers for the early effect of n-hexane on peripheral nerve injury. The feasibility of using serum NGF as a biomarker for the early effect of n-hexane on peripheral nerve injury warrants further investigation.

Detecting three halogenated hydrocarbons in blood by gas chromatography-mass spectrometry / 中国职业医学

OBJECTIVE: To establish a method for detecting dichloromethane,trichloromethane and 1,2-dichloroethane in blood by gas chromatography-mass spectrometry. METHODS: Using static headspace analysis, three halogenated hydrocarbons in blood samples were separated by DB-5 MS elastic capillary column and detected by gas chromatographymass spectrometry. RESULTS: There was a good linear relationship in the selected range of dichloromethane,trichloromethane and 1,2-dichloroethane in blood. The linear correlation coefficient was greater than 0. 999 8. The detection limit and the lower limit of quantitation was 0. 19-0. 28 and 0. 64-0. 93 μg/L,respectively. The average recovery rate was 95. 1%-106. 6%. The within-run and between-run relative standard deviation was 2. 9%-4. 9% and 5. 0%-7. 0%,respectively. The samples could be preserved at room temperature or 4 ℃ for 3 days and at-8 ℃ or below for7 days. CONCLUSION: With the features of high sensitivity,precision,accuracy,easy operation and less interference,this method is suitable for detecting dichloromethane,trichloromethane and 1,2-dichloroethane in the blood.

Development and optimization of an intergeneric conjugation system and analysis of promoter activity in Streptomyces rimosus M527 / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

An efficient genetic transformation system and suitable promoters are essential prerequisites for gene expression studies and genetic engineering in streptomycetes. In this study, firstly, a genetic transformation system based on intergeneric conjugation was developed in Streptomyces rimosus M527, a bacterial strain which exhibits strong antagonistic activity against a broad range of plant-pathogenic fungi. Some experimental parameters involved in this procedure were optimized, including the conjugative media, ratio of donor to recipient, heat shock temperature, and incubation time of mixed culture. Under the optimal conditions, a maximal conjugation frequency of 3.05×10-5 per recipient was obtained. Subsequently, based on the above developed and optimized transformation system, the synthetic promoters SPL-21 and SPL-57, a native promoter potrB, and a constitutive promoter permE* commonly used for gene expression in streptomycetes were selected and their activity was analyzed using gusA as a reporter gene in S. rimosus M527. Among the four tested promoters, SPL-21 exhibited the strongest expression activity and gave rise to a 2.2-fold increase in β-glucuronidase (GUS) activity compared with the control promoter permE*. Promoter SPL-57 showed activity comparable to that of permE*. Promoter potrB, which showed the lowest activity, showed a 50% decrease in GUS activity compared with the control permE*. The transformation system developed in this study and the tested promotors provide a basis for the further modification of S. rimosus M527.

Characteristic analysis of organic fluorosis caused by appliying of touch screen anti-fingerprint nanocoating material / 中华劳动卫生职业病杂志

Objective@#To analysis pathogenic conditions and pathogenic characteristics of organic fluorosis caused by applying of anti-fingerprint coating material on touch screen glass of the mobile phone.@*Methods@#To collect clinical data and analyze the causes and pathogenic characteristics of poisoning through surveying occupational health, detecting occupational hazards in the workplace, collecting clinical data and diagnosing of occupational diseases. 6 employees in workshop 1 of packaging were as the organic fluorine exdposed group, and 16 employees in other workshops were as the non-exposed group.@*Results@#Organic fluorine chemicals (perfluoro-1, 3-dimethylcyclohexane, hexadecafluoroheptane, perfluoro-hexane, perfluoromethy lopentane, perfluoro-2-methyl-2-pentene, etc.) can be volatilized by spraying and baking of anti-fingerprint nano-coating material on touch screen. The relative percentage of volatile components in air is 85.65%. Four cases of acute poisoning were caused by organic fluorosis deposited in a dustless air conditioning workshop with poor ventilation.The clinical manifestations of the patients were acute bronchitis, pulmonary edema and/or myocarditis. The average concentration of urine fluorine in the organic fluorine exposed group was 13.7± 4.4 mmol/mol creatinine, which was 4-5 times higher than that of other non-organic fluorine exposed groups. The difference of urine fluorine level between the organic fluorine exposed group and non exposed group was statistically significant (P<0.01) . The main indicators were abnormal for the blood oxygen saturation of finger pulse under suction air, leukocytes, neutrophils, monocytes, hypersensitivec-reactive protein, procalcitonin, l-lactate dehydrogenase, forebrain diuretic natriuretic peptide, hypersensitive troponin T in the four cases. One case was myocardial ischemia, four cases had bilateral lung symmetrically exudative lesions, one case was accompanied by a small amount of pleural pericardial effusion.@*Conclusion@#Acute organofluorine poisoning can caused by the applying of the fingerprint nano-coating material on touch screen of the mobile phone. Attention should be paid to occupational poisoning caused by the applying of the small molecular perfluoroalkanes (olefins) in new industries, new processes and new materials.

Two new compounds from rifamycin resistant mutant strain Streptomyces sp. HS-NF-1046R / 药学学报

Using silica gel column chromatography, gel chromatography and HPLC, we isolated secondary metabolites in fermentation broth of a rifamycin resistant mutation strain Streptomyces sp. HS-NF-1046R. Based on spectroscopic data, the chemical structures of three compounds were identified as 3-hydroxyl-2-N-propionyl- anthranilamide (1), 2,3-dihydro-8-hydroxy-2,2-dimethyl quinazolin-4-(1H)-one (2) and 2-aminobenzamide (3). Compounds 1 and 2, as new entities, were evaluated for cytotoxicity against A549, HepG2, HCT-116 and K562 cells using the SRB assay. Compounds 1 and 2 exhibited no cytotoxicity with IC50 over 100 μmol·L-1.

Application of fast-track surgery in thoracoscopic and laparoscopic minimally invasive esophageal cancer operation for thoracic segment esophageal cancer / 重庆医学

Objective To evaluate the influence of fast track surgery on the early outcomes of thoracoscopic and laparoscopic minimally invasive esophageal cancer operation for thoracic segment esophageal cancer.Methods The inpatients with thoracic segment esophageal squamous cancer in this hospital from January 2012 to June 2016 were retrospectively analyzed,who in the same operation group performed thoracoscopic and laparoscopic minimally invasive esophageal cancer resection,gastroesophageal left neck anastomosis and two-field lymphadenectomy.Among them,the conventional group had 156 cases from January 2012 to December 2014 and the fast track surgery group had 93 cases from January 2015 to June 2016.Their perioperative related indicators were recorded and analyzed.Results The age,sex,BMI,complications index,ASA score,tumor segment,pathological stage had no statistical difference between the fast track surgery group and conventional group;the postoperative pain score in the fast track surgery group was significantly lower than that in the conventional group(on 1 d:5.13±1.16 vs.5.69±1.17,P=0.000;on 3 d:2.63±0.76 vs.2.86±0.78,P=0.032;on 7 d:1.82±0.71 vs.2.56±0.47,P=0.005);the pneumonia occurrence rate in the fast track surgery group was much lower(7.5％ vs.17.3％,P=0.030) and sacrococcygeal skin injury was much less(4.3％ vs.12.2％,P=0.038);the occurrence rates of pulmonary atelectasi,ARDS,re-tracheal intubation,neck anastomosis fistula,atrial fibrillation and re-operation had no statistical difference between the two groups;the hospitalization stay time in the fast track surgery was shorter than that in the conventional group[(13.89 ±7.36)d vs.(17.41±6.77)d,P=0.000].Conclusion Implementing fast-track surgery measure intervention during perioperative period in the patients with thoracic segment esophageal cancer resection can alleviative postoperative pain,decreases postoperative complications and shortens the hospitalization length.

Occupational health investigate of 1-bromopropane used in a factory / 中华劳动卫生职业病杂志

Objective@#To investigate the occupational health survey of 1-brominepropane (1-BP) enterprises and understand the impact of 1-BP on the health of occupational exposure population.@*Methods@#The occupational health data of 15 1-BP workers were collected from 3 time nodes in 0 months, June and December, and the effects of occupational exposure to 1-BP on health were analyzed.@*Results@#In the workplace with pure 1-BP, the mean air concentration in the workplace was 26.8 mg/m3, and the personal contact level was 29.7 to 63.4 mg/m3. The occupational health monitoring data showed that white blood cell count (WBC) , red blood cell count (RBC) , aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were compared in 0 months, June, and 12 months, the difference was statistically significant (P<0.05) .@*Conclusion@#During the 12 months observation period, the occupational exposure to 1-BP caused the number of peripheral blood erythrocyte and leukocyte count and the level of alanine transaminase in the workers, but it did not exceed the normal reference range.

Effect of lower-limb dominance and non-dominance shuttle runs under load carriage on the balance responses in young cadets / 中国应用生理学杂志

OBJECTIVE@#This test was designed to evaluate the effect of lower-limb dominance and non-dominance shuttle runs under load carriage during different exercise load at the same exercise intensity on the balance responses.@*METHODS@#Ten healthy young males were joined in this experiment, they were (20.80±2.04) years old and (173.99±2.87) cm tall. In a randomized cross-over design, they performed four times shuttle runs under unilateral load carriage:20 m×5 at dominant side, 20 m×5 at non-dominant side, 20 m×10 at dominant side, 20 m×10 at non-dominant side respectively. Balance abilities were evaluated immediately and 20 minute post-exercise respectively, and R-R interval was recorded.@*RESULTS@#The HR, EPOC and TRIMP for all exercise load were increased significantly after shuttle runs compared to rest (0.05), which showed symmetrical change. In addition, during 20 minute recovery, the balance ability for all exercise load was returned to the rest value (>0.05).@*CONCLUSIONS@#The shuttle runs could impair the trunk control ability immediately post-exercise, the magnitude of mediolateral movement was increased as the exercise load increased. The changes of balance responses were similar between the dominant and the non-dominant side, the dominant and the non-dominant side might show cross-effects.

Impact of otrA expression on morphological differentiation, actinorhodin production, and resistance to aminoglycosides in Streptomyces coelicolor M145 / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

otrA resembles elongation factor G (EF-G) and is considered to be an oxytetracycline (OTC)-resistance determinant in Streptomyces rimosus. In order to determine whether otrA also conferred resistance to OTC and other aminoglycosides to Streptomyces coelicolor, the otrA gene from S. rimosus M527 was cloned under the control of the strong ermE* promoter. The resulting plasmid, pIB139-otrA, was introduced into S. coelicolor M145 by intergeneric conjugation, yielding the recombinant strain S. coelicolor M145-OA. As expected S. coelicolor M145-OA exhibited higher resistance levels specifically to OTC and aminoglycosides gentamycin, hygromycin, streptomycin, and spectinomycin. However, unexpectedly, S. coelicolor M145-OA on solid medium showed an accelerated aerial mycelia formation, a precocious sporulation, and an enhanced actinorhodin (Act) production. Upon growth in 5-L fermentor, the amount of intra- and extracellular Act production was 6-fold and 2-fold higher, respectively, than that of the original strain. Consistently, reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that the transcriptional level of pathway-specific regulatory gene actII-orf4 was significantly enhanced in S. coelicolor M145-OA compared with in S. coelicolor M145.