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Objective: To establish a method for the determination of quercetin and kaempferol in Ginkgo biloba extract. Methods: A simple, rapid and reproducible supercritical fluid extract (SFE) and capillary electrophoretic method were developed for it. SFE was selected by orthogonal design method. SFE conditions: temperature 60 ℃, pressure 42 mPa, static extraction 4 min, dynamic extraction volume 4 ml and 0.2 ml ethanol as modifier. MECC conditions were 55 cm?75 ?m fused silica capillary column. The running buffer contained 25 mmol/L sodium dihydrophosphate, 6.25 mmol/L sodium borate (pH= 8.5) and 35 mmol/L sodium dodecyl sulfate(SDS). The detection wave length was at 254 nm. Results: The influence of modifier on extraction rate in SFE was the most. The linear range for quercetin and kaempferol was 21.2 106.0, 20.0 100.0 ?g/ml, respectively, when cinnamic acid was in an internal standard. The recovery of quercetin for 40, 60 and 80 ?l was 93.87%, 94.02% and 94.10%, respectively. The recovery of kaempferol for 40, 60 and 80 ?l was 94.50%, 94.17% and 94.25%, respectively. Conclusion: SFE MECC is convenient and accurate method, and can be used as a measure of quality control for Ginkgo biloba extract. [
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Object To study the conditions for the extraction of active ingredients in Ligusticum chuanxiong Hort by supercritical fluid extraction (SFE) Methods Conditions for the extraction were studied by orthogonal experimental design as guided by the content of ferulic acid presented in the extract Results The optimum conditions for the yield of ferulic acid were thus established Conclusion The best SFE conditions were: temperature of the extraction pot was 70 ℃ under the pressure of 35 MPa, CO 2 as the modifier with the flow rate of 25 kg/h, the time for extraction was 2 5 h
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Objective: To establish the method of simultaneous determination of five effective constituents tanshinose Ⅱ A、danshensu、protcate-chualdehyde、puerarin and ferulic acid. Methods: After ultrasonic extraction, HPLC-DAD were used to determine the effective constituents on Zorbax SB-C 8 column with acetonitrile and pH3.0 water(adjusted with H 3PO 4) as a mobile phase. Under the gradient timetable the percent of acetonitrile increased from 5% to 72.5% in 45 min. The flow rate was 1.5mL?min -1 , Five constituents were detected at the most suitable detection wavelength. Results: The average recovery of the five effective constituents is within the range of 100.1%～106.9%(n=3), The test of the accuracy within one day shows that the RSD is less than 2.2%, and the RSD among days less than 2.8%(n=3). The test solution is stable in 10h. Conclusion: The method is accurate, simple, quick, and can be used for determining simultaneously the content of the five effective constituents in Compound Xinnaokang Soft Capsules
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A TLC condition has been developed to purify the extraction from the leaf of Torreya Arn. plant,then an optimized HPLC method was used to determine the amount of taxol.HPLC chromatographic conditions were:colum:Shim Pack CLC ODS(150 mm?6.0 mm,5 ?m);mobile phase:methanol water(66∶34);flow rate: 0.5 ml/min;wavelength of the detector: 227 nm;colum temperature: 35℃.Using this method,we have determined the trace level of taxol in the leaf of Torreya Arn .plant successfully.There exists better linear relationship between peak height( Y ) and the concentration of detected sample( X ) within 100 500 ?g/ml.The average recovery rate is 85.7%( n =12);RSD is 7.8%.