RESUMEN
Objective: To investigate the association between polymorphisms of the C-106T at promotor region of aldose reductase (AR) gene in chromosome 7q35 and nephropathy with type 2 diabetes in people of Han nationality in North China. Methods: A case-control study in 202 Chinese subjects (including 139 type 2 diabetes mellitus with or without nephropathy and 63 norma1 control) was performed. The genotypes and alleles of polymorphisms of C-106T at promotor region of AR gene were determined by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method with 4% agarose gel electrophoresis. The results were validated by sequencing the PCR products. Results: The frequency of the T allele and CT+TT genotypes at promotor region of AR gene was significantly higher in DN+ group than that in DN-group and control (CON) group (P0.05). Conclusion: The T allele and CT/TT genotypes at promotor region of AR gene may related to nephropathy with type 2 diabetes in people of Han nationality in North China.
RESUMEN
Objective:To investigate the association between polymorphisims of both the 894G→T at exon 7 of endothelial nitric oxide synthase(eNOS) gene and the C-106T at promotor region of aldose reductase(AR) gene and type 2 diabetic nephropathy(DN) of Chinese. Methods: A case-control study for 202 Chinese subjects was performed.The genotypes and alleles of polymorphisims of 894G→T at exon 7 of eNOS gene and C-106T at promotor region of AR gene were determined by polymerase chain reaction restriction fragment lenth polymorphism method(PCR-RFLP). Results:The frequency of the T allele and TG genotype at exon 7 of eNOS gene and the T allele and CT genotype at promotor region of AR gene were significantly higher in DN+ group than that in DN-group and control group(P0.05).The type 2 diabetes with coexistence of the T allele/TG genotype of eNOS gene and T allele/CT genotype of AR gene had higher incidence of DN(P