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1.
Chinese Journal of Biotechnology ; (12): 3125-3142, 2023.
Artículo en Chino | WPRIM | ID: wpr-1007949

RESUMEN

C1 gases including CO, CO2 and CH4, are mainly derived from terrestrial biological activities, industrial waste gas and gasification syngas. Particularly, CO2 and CH4 are two of the most important greenhouse gases contributing to climate change. Bioconversion of C1 gases is not only a promising solution to addressing the problem of waste gases emission, but also a novel route to produce fuels or chemicals. In the past few years, C1-gas-utilizing microorganisms have drawn much attention and a variety of gene-editing technologies have been applied to improve their product yields or to expand product portfolios. This article reviewed the biological characteristics, aerobic or anaerobic metabolic pathways as well as the metabolic products of methanotrophs, autotrophic acetogens, and carboxydotrophic bacteria. In addition, gene-editing technologies (e.g. gene interruption technology using homologous recombination, group Ⅱ intron ClosTron technology, CRISPR/Cas gene editing and phage recombinase-mediated efficient integration of large DNA fragments) and their application in these C1-gas-utilizing microorganisms were also summarized.


Asunto(s)
Edición Génica , Gases , Dióxido de Carbono , Ingeniería Genética , Clonación Molecular
2.
Microbiology ; (12)2008.
Artículo en Chino | WPRIM | ID: wpr-595960

RESUMEN

Electricigens play an important role in microbial fuel cell(MFC) . This review provides an introduction of different electricigens on theirs taxonomical group,biochemical,physiological and morphological characteristics. The ability of electricity production of electricigens and electron transfer mechanisms in microbial fuel cells are also concluded. The prospect of waste water treatment and bio-electricity production is underlined,it is point out in this review that the future research of microorganism for MFC should be focused on enrichment,adaptation,modification and optimization by multi-strains application to improve the performances of MFC.

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