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Objective To assess the effects of gelatine and 6% hydroxyethyl starch 200/0.5 (HES 200 / 0.5) on phagocytic activity of human neutrophils and monocytes using flow cytometry.Methods Thirty-three ASA Ⅰ - Ⅱ patients aged 18-70 years scheduled for urological minor surgery were randomly divided into three equal groups of eleven patients :group I gelatine;group II HES 200 / 0.5 and group Ⅲ lactated Ringer's solution (LR) . 10 ml?kg-1 of gelatine, HES or LR was infused over 60 min and venous blood samples were taken before infusion and 1 h after the start of infusion for determination of phagocytes with ingested FITC-labeled E coli by flow cytometry. Results In gelatine group the percentage of neutrophils and monocytes with phagocytic activity decreased significantly after infusion ( P
RESUMEN
Objective To assess the changes in blood coagulation after infusion of hydroxyethyl starch 45/ 0.7(molecular weight = 450000 Dalton, 70% of glucose units have been substituted) ,200/0.62, 200/0.5 and 130/0.4.Methods Forty rabbits weighing (2.6 ?0.5)kg were randomly divided into 5 groups of 8 animals each: group I HES450/0.7; group II HES 200/0.62; group III HES200/0.5; group IV HES 130/0.4; group V 0.9% NaCl. The animals were anesthetized with intramuscular seconal and ketamine. Hydroxyethyl starch or normal saline was infused at 10 ml?kg-1?h-1 for 3 h. Blood samples were taken before and 1,2,3 h after staring the infusion for determination of prothrombin time ( PT), activated partial thromboplastin time (APTT) and fibrinogen level (FIb) and thromboelastograph examination (TEG) .Results (1) After infusion of 30 ml?kg-1 HES 450/0.7 or 200/0.62 (in group I and II ) R time (representing the rate of initial fibrin formation) and K time (coagulation time) were significantly prolonged (P