Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway / 中国结合医学杂志

OBJECTIVE@#To investigate the effects of asperuloside on cervical cancer based on endoplasmic reticulum (ER) stress and mitochondrial pathway.@*METHODS@#Different doses (12.5-800 µg/mL) of asperuloside were used to treat cervical cancer cell lines Hela and CaSki to calculate the half maximal inhibitory concentration (IC50) of asperuloside. The cell proliferation was analyzed by clone formation assay. Cell apoptosis, intracellular reactive oxygen species (ROS) and mitochondrial membrane potential were determined by flow cytometry. The protein expressions of cleaved-caspase-3, Bcl-2, Bax, Cyt-c, cleaved-caspase-4 and glucose-regulated protein 78 (GRP78) were analyzed by Western blot. And the inhibitor of ER stress, 4-phenyl butyric acid (4-PBA) was used to treat cervical cancer cells to further verify the role of ER stress in the apoptosis of cervical cancer cells induced by asperuloside.@*RESULTS@#Asperuloside of 325, 650, and 1300 µg/mL significantly inhibited the proliferation and promoted apoptosis of Hela and CaSki cells (P<0.01). All doses of asperuloside significantly increased intracellular ROS levels, reduced mitochondrial membrane potential, significantly reduced Bcl-2 protein expression level, and increased Bax, Cyt-c, GRP78 and cleaved-caspase-4 expressions (P<0.01). In addition, 10 mmol/L 4-PBA treatment significantly promoted cell proliferation and reduced apoptosis (P<0.05), and 650 µg/mL asperuloside could reverse 4-PBA-induced increased cell proliferation, decreased apoptosis and cleaved-caspase-3, -4 and GRP78 protein expressions (P<0.05).@*CONCLUSION@#Our study revealed the role of asperuloside in cervical cancer, suggesting that asperuloside promotes apoptosis of cervical cancer cells through ER stress-mitochondrial pathway.

Therapeutic effect of deproteinised calf serum eye gel on corneal alkali burn / 中华实验眼科杂志

Background Corneal chemical burns,especially hazards of alkali burn become increasingly prominent.Clinically,anti-inflammatory,immuno-suppression,corneal transplantation are the common treating method for corneal alkali burn.But the research of tissue repair under the microenvironment of corneal alkali burn is necessary.Objective The aim of this study was to investigate the therapeutic effect of deproteinised calf serum eye gel on the corneal alkali burn.Methods Alkali burn model of cornea was established on the right eyes by putting the filter paper with 0.5 mol/L NaOH on the center cornea for 1 minute in 24 white rabbits.The model rabbits were divided randomly into 4 groups.Normal saline solution,deproteinised calf serum eye gel,blank matrix gel or recombinant bovine basic fibroblast growth factor(rb-bFGF)eye-gel was topically administered 4 times per day for 14days in the 4 groups,respectively.The inflammatory reaction was examined under the slit lamp and scored based on Ando' s criteria.Corneal fluorescine staining was performed to calculate the corneal ulcer area and scored based on Trousdale' s criteria.Histopathological examination of corneas was performed on the fourtcenth day after experiment.The use of the experiment animals complied with ARVO Statement.Results Corneal edema and opacification were seen in the model eyes with the modeling successful rate 100％.On the seventh day after experiment,the severe ulcer of cornea and hypopyon appeared in the normal saline solution group.Corneal epithelium was intact but the intrarocular structure was invisible in the blank matrix gel group.In th(c) rb-bFCF group,corneal new vessels were seen,however,the corneal ulcer completely regrow in the deproteinised calf serum eye gel group.In 3,5,7,10 and 14days after examination,the corneal inflammatory scores were significantly lower in the deproteinised calf serum eye gel group and rb-bFGF group than those of the normal saline solution(P＜0.01).No significant difference was found in the inflammatory score between the deproteinised calf serum eye gel group and rb-bFGF group (P＞0.05) but was significantly lower than the blank gel matrix group (P ＜ 0.05).With respect to the corneal ulcer,the score was decreased in the deproteinised calf serum eye gel group compared with the normal saline solution group and blank gel matrix group (P ＜ 0.05).Howcver,no significant difference was found in the corneal ulcer score between the deproteinised calf serum eye gel group and rb-bFGF group in various time points (P＞ 0.05).Conclusions Deproteinised calf serum eye gel can promote the healing of corneal ulcer and remit the inflammatory response afler corneal alkali burns with a better effectiveness than rb-bFGF.