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Objective To compare the performance of 2 kinds of quantitative detection method the chemiluminescence immu-moassay and the enzyme-linked immunosorbent assay(ELISA)for detecting serum human epididymis geneproduct 4(HE4),and to explore the accuracy and practical value of the two methods.Methods In accordance to the document EP9-A2 of NCCLS,the Roche automatic electrochemical immunoassay system as the comparative method(X)and the ELISA as the testing method(Y )were per-formed according to the requirements of the detection kits.Results The serum HE4 level could be accurately reflected by the two methods.The correlation coefficient(r)in comparing these two kinds of method was ≥0.975,which showed that there was a high correlation between them.The detection results of the two methods were compared by paired sample t-test,and no statistically sig-nificant difference was found between them(P >0.05).Conclusion Because of CLIA′88 giving no specific medical decision level of HE4 and clinical acceptable level,therefore the expected bias of credibility interval can not be calculated or whether being clinically acceptable is unable to be judged.Based on the comparison of paired sample t test,these two kinds of detection methods can be used interchangeably to a certain extent.
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The experiment was carried out to inquire about the possibility of strain selection and improvement in Lentinus edodes through protoplast formation and mutagenesis by UV. Mycelial growth rate, yield, and period of fructification of 105 selected regenerants from UV-treated protoplasts have been measured and compared with their parents. Several selected regenerants achieved some better qualities such as higher yield and earlier fruiting. Several repeated cultivations of these regenerants reconfirmed their new capacities. The results indicated that protoplast formation and mutagenesis are the potential methods for strain selection and improvement in edible fungi.
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This paper is about the research on the growth of jyphal body of Hypsizygus marmoreus under the condition of different culture mediums, tempertures, pH, cardon and nitrogen sources. The result shows that Hypsizygus marmoreus can grow on various culture mediums. It grows best on the Culture medium of malt extract agar, but can not grow well on the PDA composite culture medium; the hyphal body can grow well at the temperture of 20—25℃, grows slowly at 10—30℃, but can not grow at 35℃; it can grow when pH is 5 to 8, but the best range of pH is 6.5—7.0; the nutriment assimilation is different with the different sources of carbon and nitrogen, the best carbon source is maltose, best nitrogen source is bacto-peptone.
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The new technology had been introduced that Agaricus bisporus had been cultivated in summer in the North of China by using of corn stalks in simple mushroom house. The result was satisfactory that biological efficience for corn stalks was 48% and the yield was 9.6 kg per squire meter.