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Objective: To investigate the effect of oxLDL on CD36 expression and cholesterol influx in THP-1 macrophage. Methods: THP1 macrophage were treated with 50mg/L oxLDL for 0,12,24,36,48 h respectively.Oil red O staining was used to observe the intracellular lipid droplets.\labeled Cholesterol influx was determined by FJ-2107P type liquid scintillator.CD36 mRNA and protein level were determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting respectively. Results:We found that oxLDL elevated CD36 in both protein and mRNA levels and increased cholesterol influx in a time-dependent manner.The levels of cholesterol influx were 23.5%、(27.8%、)39.7%、44.1% and 49.7% respectively. Conclusion: oxLDL may upregulate CD36 expression and increase cholesterol influx.
RESUMEN
AIM: To determine the effect of promoting cellular cholesterol efflux on the apoptosis of foam cells derived from monocytes. METHODS: RAW264.7 cells were incubated with 50 mg/L ox-LDL as a foam cell mode. The apoptosis rate of RAW264.7 cells was assayed by flow cytometry. Cellular lipid droplet was assayed by oil red staining. The rate of cellular cholesterol efflux was assayed with [~3H] label cholesterol, and the content of cellular cholesterol were assayed by high performance liquid chromatography. RESULTS: After incubation with 50 mg/L ox-LDL for 48 h, the content of cellular cholesterol ester increased from (6.8?3.6) mg/g to (101.7?4.5) mg/g (P