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Objective:To explore the diagnostic value of chest CT imaging in differential diagnosis between common-type COVID-19 and mycoplasma pneumonia (MP).Methods:From the January to February 2020, the clinical and imaging data of COVID-19 patients (diagnosed in the Affiliated Hospital of Jining Medical University, the Fourth People's Hospital of Jining and the Second People's Hospital of Jining) and MP patients (diagnosed in the Affiliated Hospital of Jining Medical University) were retrospectively collected and analyzed. Forty-three patients with common-type COVID-19 (28 males, 15 females, 43±14 years old) and 50 patients with MP (19 males, 31 females, 37±14 years old) were enrolled as COVID-19 group and MP group, respectively. The clinical manifestations, laboratory results and chest CT findings of these two groups were analyzed and compared.Results:(1) Clinical manifestations: there were more patients with muscle ache and asthenia in COVID-19 group than in MP group (χ 2=5.110, 4.834, P<0.05). No significant difference was found in fever and cough between two groups (χ 2=0.378, 0.097, P>0.05). (2) Laboratory examination: the procalcitonin level of cases in COVID-19 group was significantly lower than that in MP group (χ 2=12.263, P=0.001). No significant difference was found in leukocyte count, lymphocyte count, C-reactive protein level and erythrocyte sedimentation rate ( Z=-1.117, χ 2=2.410, 0.787, 0.800, all P>0.05) between two groups. (3) Chest CT findings bilateral lung involvement was found more in COVID-19 group than in MP group (χ 2=30.012, P<0.001); while the one lobe of ipilateral lung involvement was less in COVID-19 group than in MP group (χ 2=19.927, P<0.001); there was no significant difference in multiple lobes of ipilateral lung involvment between the two groups (χ 2=1.366, P>0.05). Ground glass, paving stone sign and air bronchus sign were found significantly more in COVID-19 group than in MP group (χ 2=30.171, 19.119, 9.790, all P<0.05); while the pulmonary consolidation, central lobular nodule and centripetal thickening of bronchus wall were found significantly less in COVID-19 group than in MP group (χ 2=25.450, 33.532, 48.553, all P<0.001). Conclusions:The clinical manifestations and laboratory examination have limited value in the differential diagnosis of common-type COVID-19 and MP, while chest CT imaging might be more valuable in the early differential diagnosis of these two diseases.
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Objective To investigate the protective effect and mechanisms of thymosin beta 4 (Tß4) on oxygen-glucose deprivation/reoxygenation (O G D / R) injury in rat cortical neurons. Methods Primary cultured cortical neurons were isolated and identified; Ischemia-reperfusion injury model of rat cortical neurons was established (oxygen glucose deprivation 6 h, reoxygenation 12 h) by OGD / R. The rats were divided into the control group,the model group and the treatment group (addition of thymosinß4 2 h before modeling); The cell counting Kit-8 (CCK8) was used to determine the optimal concentration of Tß4. Flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were used to detect apoptosis. Western blotting was used to detect the expression of 78 000 glucose-regulated protein 78 (GRP 78), CCAAT/enhancer binding protein-homologous protein (CHOP), B-cell lymphoma-2 (Bel-2) and Bax. Expression levels were compared among groups. One-way analysis of variance was used to compare the normally distributed measurement data among three groups with the SPSS19. 0 software. Results Cortical neurons were separated correctly. The optimal concentration of Tß4 was 10 (ig/L. Comparing the model group with the control group, the survival rate of cortical neurons decreased significantly (P = 0. 002), the apoptotic rate increased significantly (P < 0. 01), the expression of GRP78,CHOP and Bax was up-regulated significantly (P values were 0. 034,0 and 0. 045 .respectively),and the expression of Bcl-2 was reduced significantly (P = 0. 006). Comparing the treatment group(10 p.g/L exogenous Tß4) with the model group, cell viability increased significantly (P = 0. 008), the apoptotic rate decreased significantly (P = 0. 002),the expression of GRP78,CHOP and Bax decreased significantly (P values were 0. 032,0. 027 and 0. 019, respectively),and the expression of Bcl-2 increased significantly (P = 0.028) .The differences were statistically significant. Conclusions Tß4 inhibits OGD/R-induced endoplasmic reticulum stress-dependent apoptosis. These results provide a theoretical basis for the application of Tß4 in the treatment of cerebral ischemia-reperfusion injury.