RESUMEN
Objective:To evaluate the value of radiomics based on contrast-enhanced spectral mammography (CESM) of internal and peripheral regions combined with clinical factors in predicting benign and malignant breast lesions of breast imaging reporting and data system category 4 (BI-RADS 4).Methods:A retrospective analysis was performed on the clinical and imaging data of patients with breast lesions who were treated in Yantai Yuhuangding Hospital (Center 1) Affiliated to Qingdao University from July 2017 to July 2020 and in Fudan University Cancer Hospital (Center 2) from June 2019 to July 2020. Center 1 included 835 patients, all female, aged 17-80 (49±12) years, divided into training set (667 cases) and test set (168 cases) according to the "train-test-split" function in Python software at a ratio of 8∶2; and 49 patients were included from Center 2 as external validation set, all female, aged 34-70 (51±8) years. The radiomics features were extracted from the intralesional region (ITR), the perilesional regions of 5, 10 mm (PTR 5 mm, PTR10 mm) and the intra-and perilesional regions of 5, 10 mm (IPTR 5 mm, IPTR 10 mm) and were selected by variance filtering, SelectKBest algorithm, and least absolute shrinkage and selection operator. Then five radiomics signatures were constructed including ITR signature, PTR 5 mm signature, PTR 10 mm signature, IPTR 5 mm signature, IPTR 10 mm signature. In the training set, univariable and multivariable logistic regressions were used to construct nomograms by selecting radiomics signatures and clinical factors with significant difference between benign and malignant BI-RADS type 4 breast lesions. The efficacy of nomogram in predicting benign and malignant BI-RADS 4 breast lesions was evaluated by the receiver operating characteristic curve and area under the curve (AUC). Decision curve and calibration curve were used to evaluate the net benefit and calibration capability of the nomogram.Results:The nomogram included ITR signature, PTR 5 mm signature, PTR 10 mm signature, IPTR 5 mm signature, age, and BI-RADS category 4 subclassification for differentiating malignant and benign BI-RADS category 4 breast lesions and obtained AUCs of 0.94, 0.92, and 0.95 in the training set, test set, and external validation set, respectively. The calibration curve showed good agreement between the predicted probabilities and actual results and the decision curve indicated a good net benefit of the nomogram for predicting malignant BI-RADS 4 lesions in the training set, test set, and external validation set.Conclusion:The nomogram constructed from the radiomics features of the internal and surrounding regions of CESM breast lesions combined with clinical factors is attributed to differentiate benign from malignant BI-RADS category 4 breast lesions.
RESUMEN
Objective To compare the efficacy and safety of ultrasound guided pectoral nerves Ⅱ (Pecs Ⅱ) block with thoracic paravertebral nerve (TPVN) block for postoperative analgesia after modified radical mastectomy.Methods Eighty female patients scheduled for radical mastectomy,aged 40-65 years,ASA physical status Ⅰ or Ⅱ,were randomly divided into 2 groups using a random number table method (n =40 each):the patients in group T received TPVN block,whereas the pa tients in group P received Pecs Ⅱ block.Both the groups received 0.5 ％ ropivacaine 25 ml.The blocks were performed under all aseptic precautions in the preoperating room 30 min before surgery.The total number of dermatomes that had less pain to pin prick compared with opposite side were not ed.All patients were observed for 30 min after performing the block.The patients were received patient-controlled intravenous analgesia (PCIA).The duration of analgesia and total analgesic consumption in 24 h after surgery were recorded.Adverse effects were recorded between the two groups.Results The duration of analgesia in group P was significantly prolonged than group T [(326.5± 47.8) min vs (201.4±34.5) min,P＜0.01].The 24 h sufentanil consumption were also decreased in group P [(6.9±1.2) μg vs (10.7±1.9) μg,P＜0.01].T2 dermatomal spread were significantly increased in group P [35 (87.6％) cases vs 9 (22.5％) cases,P＜0.05].No complication was recor ded.Conclusion Ultrasound-guided Pecs Ⅱ block and TPVN provided safe and effective anesthesia in patients undergoing modified radical mastectomy,but the effect of Pecs Ⅱ block were more satisfied and per sistent.
RESUMEN
Objective To explore molecular mechanisms of dexmedetomidine(Dex)function as protec-tive effect on acute lung injury(ALI). Methods Hemorrhagic shock ALI rat model was constructed and divided into 6 groups at random:C group,control group;A group,ALI;G,ALI rat groups were treated with 5μg/kg;I1 group,ALI+PDTC(200 mg/kg);I2 group,ALI+STA-21(100 mg/kg). After 12 h modeled,all rats were anes-thetized and killed,and BALF was collected and the concentrations of TNF-α,IL-6,IL-1β,IL-4 were deter-mined using ELISA. HE staining to observe the morphology of lung tissue. Expression of NF-κB,STAT-3,p-STAT-3 proteins were determined using immunohistochemistry or Western blot. Results In A group,the structure of lung tissue were strongly destroyed ,a large number of neutrophils invasion and red blood cells leakage was found;lung tissue structure in G,I1 and I2 groups were gross integrity. The concentrations of TNF-α,IL-6,IL-1β,IL-4 in A group were significantly higher than C group(P<0.05). The concentrations of TNF-α,IL-6,IL-1β,IL-4 in G group,I1 group and I2 group was significantly lower than A group(P<0.05). The levels of NF-κB and p-STAT-3 protein in A group were obvious enhanced(P < 0.05)compared with G group,I1 group,I2 group,respectively. Conclusion Dex inhibited inflammatory to exert lung protective effect via inhibiting NF-κB,STAT-3 activation-induced by ALI.
RESUMEN
OBJECTIVE:To investigate the effects of preemptive analgesia of parecoxib-sodium for radical mastectomy of breast cancer. METHODS:A total of 60 female patients underwent selective radical mastectomy of breast cancer under general anes-thesia were randomly divided into observation group(40 cases)and control group(20 cases). 15 min before anesthesia induction, observation group was given intravenous injection of Parecoxib-sodium for injection 40 mg. Control group was given intravenous in-jection of 0.9% Sodium chloride injection 5 mL. The pain visual analogue scale(VAS),the frequency of patient controlled intrave-nous analgesia(PCA)and ADR were observed between 2 groups at different time points after surgery. RESULTS:VAS scores of observation group were significantly lower than those of control group 2,4,6,8 h after operation,and the frequency of PCA 0-4, 4-12,12-24,24-36 h after operation was significantly lower than control group,with statistical significance(P0.05). CONCLUSIONS:The preemptive analgesia of parecoxib-sodium can effectively reduce pain degree of patients with breast cancer after radical mastectomy,the frequency of PCA, and do not increase the occurrence of ADR.
RESUMEN
Avian influenza virus is a new recombinant virus , which can cause severe respiratory symptoms ,such as short course,acute disease,and a high mortality rate.The purpose of this paper was to summarize the current status of this virus in terms of its epidemiology ,genetic evolution and virulence .By introducing the advancement in the research of this subtype virus, we hope to provide data and evidence ,for effective surveillance and prevention of this virus .
RESUMEN
Objective To explore molecular mechanisms of dexmedetomidine(Dex)function as protec-tive effect on acute lung injury(ALI). Methods Hemorrhagic shock ALI rat model was constructed and divided into 6 groups at random:C group,control group;A group,ALI;G,ALI rat groups were treated with 5μg/kg;I1 group,ALI+PDTC(200 mg/kg);I2 group,ALI+STA-21(100 mg/kg). After 12 h modeled,all rats were anes-thetized and killed,and BALF was collected and the concentrations of TNF-α,IL-6,IL-1β,IL-4 were deter-mined using ELISA. HE staining to observe the morphology of lung tissue. Expression of NF-κB,STAT-3,p-STAT-3 proteins were determined using immunohistochemistry or Western blot. Results In A group,the structure of lung tissue were strongly destroyed ,a large number of neutrophils invasion and red blood cells leakage was found;lung tissue structure in G,I1 and I2 groups were gross integrity. The concentrations of TNF-α,IL-6,IL-1β,IL-4 in A group were significantly higher than C group(P<0.05). The concentrations of TNF-α,IL-6,IL-1β,IL-4 in G group,I1 group and I2 group was significantly lower than A group(P<0.05). The levels of NF-κB and p-STAT-3 protein in A group were obvious enhanced(P < 0.05)compared with G group,I1 group,I2 group,respectively. Conclusion Dex inhibited inflammatory to exert lung protective effect via inhibiting NF-κB,STAT-3 activation-induced by ALI.
RESUMEN
Objective To investigate the impact of enhanced recovery after surgery (ERAS) program on postoperative recovery in patients undergoing laparoscopic colorectal resection. Methods Eighty-four patients undergoing laparoscopic colorectal resection from March 201 5 to June 201 6 (55 males,29 females,aged 36-78 years,ASA physical status Ⅰ or Ⅱ),were randomly divid-ed into two groups (n = 38 each).Patients in group E were received epidural block combined with general anesthesia,and a series of perfect ERAS strategies,such as strengthen preoperative educa-tion, maintaining perioperative normothermia, perioperative goal-directed fluid therapy, intraoperative and postoperative analgesia.While the patients in group C received routine anesthetic management.The volume of fluid,the nasopharyngeal temperature,the time of recovery of bouel sound,first anal exhaust,eating fluid food,ambulation and remove of the catheter were recorded in two groups.Furthermore,time of PACU after surgery,the total days of hospitalization and total hos-pital costs were recorded.Results The volume of fluid [(1 328 ± 64)ml vs.(2 463 ± 135 )ml]in group E were significantly lower than group C (P <0.05),the nasopharyngeal temperature [(36.2± 0.2)℃ vs.(35.1±0.5)℃]was significantly higher in group E (P <0.05).Compared with group C,the time of recovery of bowel sound [(33.4 ± 12.5 )h vs.(42.8 ± 14.3 )h],first anal exhaust [(43.6±13.9)h vs.(60.7±1 5.4)h],eating fluid food [(26.8±4.1)h vs.(67.4±13.5)h],first ambulation [(7.4±1.6)h vs.(26.5±3.8)h]and remove of the catheter [(29.2±6.1)h vs.(5 1.8 ±7.6) h ], time of PACU [(26.4 ± 8.5 ) min vs.(37.2 ± 1 1.6 ) min ], the total days of hospitalization [(7.5±0.9)d vs.(9.7±1.2)d]were significantly shorter (P <0.05),and hospital costs [(2.1±0.6)ten thousand yuan vs.(2.6±0.8)ten thousand yuan]were significantly decreased (P <0.05).The incidence of adverse reactions such as nausea and vomiting (2.4% vs.21.4%),pru-ritus (7.1% vs.23.8%),agitation (4.8% vs.26.2%)and chills (0% vs.1 9.0%)were significantly lower in group E (P <0.05).Conclusion ERAS program applied to patients undergoing laparoscopic colorectal resection can reduce the intraoperative sufentanil consumption,avoid the occurrence of postoperative hypothermia, accelerate recovery of gastrointestinal function, which can obviously reduce the hospitalization costs and shorten the hospitalization time.
RESUMEN
Objective To compase laparoscopic vs open appendectomy in the treatment of appendicitis.Methods The clinical data of 1 716 appendicitis patients are studied in the form of retrospective summary,who were hospitalized in Jilin University China-Japan Union Hospital from 2009 to 2014.Patients were divided into laparoscopic appendectomy group and open appendectomy group.Duration of operation,surgical complications and postoperative recovery were evaluated.The chi-test and the student t test were used for statistics.Results More patients in open group with chronic lung disease,or a history of previous abdominal surgery than in the laparoscopic group (x2 =3.527,22.804,P ＜ 0.05);the postoperative deep vein thrombosis,intestinal obstruction,abdominal abscess and infection of incision in open group were more often seen than in laparoscopic group (x2 =4.179,4.71,7.351,8.766,P ＜ 0.05);Open group scored higher than laparoscopic group on the index of 24 hours of postoperative pain (t =-3.163,P =0.004);duration of surgery was similar (t =1.754,P =0.092 2);the laparoscopic group was better in recovery time of postoperative intestinal peristalsis,average length of scar and the average hospital stay (t =3.460,-15.335,-3.629,P ＜ 0.05).While the average hospitalization cost in open group is less than in the laparoscopic group (t =5.763,P =0.001).Conclusions Both laparoscopic appendectomy and open appendectomy were effective for the treatment of appendicitis,while laparoscopic appendectomy is superior to open procedure in reducing postoperative pain,postoperative complications promoting rapid recovery,shortening hospital stay and more cosmetic.
RESUMEN
Detection of pathogenic microorganisms has been a hot research field of microbiology.Conventional detection methods,such as isolation and culture, PCR technology, ELISA and genomic sequencing,are all time-consuming and com-plex.Because of the advantages of quick-testing, accuracy, safety and efficiency, spectroscopy has become a new non-inva-sive testing technology and has witnessed rapid development in pathogen detection and disease diagnosis.This article intro-duces three types of common spectroscopy technologies ( laser excitation fluorescence spectroscopy, infrared spectroscopy and Raman spectroscopy) , and also explains how they work in the detection of pathogenic microorganisms.
RESUMEN
Ebola virus disease , which can cause ebola hemorrhagic fever , is a potent zoonotic infectious virus disease . In 2014 , Ebola virus spread across West Africa and it has become a new major threat to global public health .This article summarizes the structural features of Ebola virus , transmission characteristics , interactions ,animal models .
RESUMEN
BACKGROUND:Recently,with the rapid development of material science and bioscience,the technology of dental implant has made great progress,especially the immediate implant technology.But the size and shape of implant are usually not fit for tooth extraction wound,so it is an important factor that leads to failure when implant and tooth extraction wound can not form close tangency.Guided bone regeneration or bone grafting materials are usually used to solve this problem.OBJECTIVE:To study the effects of nano-hydroxyapatite/collogen (nHAC) with recombinant human bone morphogenetic protein-2(rhBMP-2) and titanium (Ti) membrane on repairing peripheral bone defects of instant implant.DESIGN,TIME AND SETTING:A randomized,controlled animal study was performed at the Central Laboratory,the Fourth Military Medical University of Chinese PLA between January 2005 and January 2006.MATERIALS:Ti screw implants (diameter 2 mm,length 10 mm,and pitch 0.4 mm) without the part that went through gum were offered by Nonferrous Metal Academy in Baoji,China.The nonabsorbable Ti membranes (2 cm×2 cm) were offered by Zhongbang Biomaterial Limited Company in Xi'an,China.The nHAC materials were gifted by professor Cui Fu-zhai from Material Science and Engineering Department of Tsinghua University and fabricated into 0.5 mm×0.5 mm×0.5 mm small blocks.rhBMP-2 was offered by the Academy of Military Medical Sciences in Beijing,China.rhBMP-2 was dissolved with hydrochloric carbamidine and then nHAC was immersed in it.Vacuumization,freeze-drying,and Ekibon degermation were followed.Each gram of nHAC compounds required approximately 1 mg rhBMP-2.METHODS:Four healthy purebred male dogs were included in this study.According to the methods to repair bone defects rhBMP-2+Ti membrane,nHAC composited with rhBMP-2 was implanted,covering Ti membrane.Six defects were made on the mandible on each side.MAIN OUTCOME MEASURES:At 6 and 12 weeks after implantation,new bone formation and the correlation of new bone to Ti implant were investigated by radiography,bone density measurement and histological examinations.RESULTS:All the bone wounds were full of new bone at 12 weeks,the Ti implants were steady and the osseointegration was good.In the nHAC+rhBMP-2 and nHAC+rhBMP-2+Ti membrane groups,new bone formed and maturated earlier.In the nHAC +Ti membrane,blank control,and nHAC + rhBMP-2 +Ti membrane groups,new bone formed well below Ti membrane and the alveolar crest was plump.In the nHAC + rhBMP-2 +Ti membrane group,new bone formed earliest with the most quantity and the alveolar crest was the plumpest.By 12 weeks,the bone density had achieved the same level as the autologous cancellous bone transplantation,but the alveolar crest was plumper compared to the positive control group.In addition,bone mass was much more.Bone formed slowest in the blank control group and fastest in the positive control group with the crest of ridge being slightly absorbed.CONCLUSION:nHAC is a new good bone graft material in repairing peripheral bone defects of instant implant,especially compositing with rhBMP-2 and/or Ti membrane.A round method to repair the surrounding bone defect of instant implant should be selected according to the specific situation in the clinic.
RESUMEN
Objetive:To detect the gene mutation of a patient with anhidrotic ectodermal dysplasia. Methods: Genomic DNA was extracted from the peripheral blood of the patient and all the exon fragments were obtained by PCR. These fragments were sequenced directly. Results: In EDA gene a novel base transition was detected: -48A→G. Conclusion: There is a new base transition in EDA gene in this patient
RESUMEN
Objective:To study the sensitization of two newly developed titanium alloys, TLE and TLM.Methods:According to ISO 10993-10:1995 standard,maximization test was conducted in guinea pig.The skin sensitization reactions,including erythema and oedema, induced by TLE, TLM, normal saline and 2,4-dinitrochlorobenzene were observed and scored respectively at 24,48 and 72 h exposure of the infusion of the materials. The allergenic rates and mean response score were calculated.Results:The allergenic rates and score of skin reaction of TLE and TLM were 0/15 and 0,those of normal saline 0/15 and 0,those of 2,4-dinitrochlo-robenzene 15/15 and 5,respectively.Conclusion: TLE and TLM both are not of sensitization.
RESUMEN
0.05,group 1 or group 2 vs group 3 P
RESUMEN
Objective: To study the ionic diffusion and the distri bu tion character in the interfacial area of aurogalvano form ceramic crown, and t o discuss the bond mechanism of porcelain fused to 99.9% gold. Method s: The ionic diffusion and the distribution character in the interfac ial area of aurogalvano form ceramic crown were observed by electron probe and scanning electron microscope. Results: The examination wit h electron microprobe showed that from gold to porcelain aluminum ascended from zero to peak value, aurum dropped from the peak value to zero, and the bandwidt h was about 3～4 ?m.There were obvious mechanical table and tuber. C onclusion: There is ionic diffusion and no crack in the interfacial a rea;there are physical and chemical combinations between porcelain and 99.9% gol d.
RESUMEN
Objective: To investigate the influence of repeated infiltrating on the microstucture of In-Ceram alumina substrate. Methods:6 disks of aluminum oxide with the height of 3 mm and diameter of 12 mm were perpared and infiltrated with infiltrating glass for 0～5 times for each disk respectively.The fractured surfaces of the samples were observed by scanning elctron microscope. Results:After sintering,a net -shaped porous coping of alumina was formed. The glass infiltration resulted in a dense ceramic mass. The porous coping matrix was infiltrated with low-viscosity glass in void.3-time-infiltration resulted in the best results. Conclusion: With repeated infiltrating firing,the micro-stucture of In-Ceram alumina substrate may become a dense mass.
RESUMEN
objective: To investigate the effects of cerium oxide content on the color of alumina glass composite. Methods: Infiltration glass powder with different content of cerium oxide (1%～4%) were fabricated. Alumina glass composite was prepared by infiltrating molten glass into presintered alumina block at 1 250 ℃ for 2 h, and the color of the alumina glass composite were measured with Minolta chromatic instrument(CR 321) . For comparison, the color of VITA In Ceram alumina technical shade guide was recorded too. Results: After infiltration, the b * range of the alumina glass composite was in accord with that of the shade guide. With the increasing content of the cerium oxide, a *, b * and chroma increased, L * decreased, the hue of the alumina glass composite shifted from yellow green to yellow red. Conclusion: Cerium oxide may effectively enhance b * of the alumina glass composite, but its ability of reducing L * and enhancing a * is weak.
RESUMEN
Objective: To study the influence of repeated firings on the PFM bond strength. Methods: 25 specimens of PFM in the diameter of 13.0 mm and thickness of (1.0?0.02) mm were prepared and fired at 920 ℃ for 1, 3, 5, 7 or 9 times(5 samples for each time). The remained porcelain rate(RPR) was measured by Mackert method and used to evaluate the bond strength.Results: After 3 firings, the RPR of PFM decreased obviously, and additional firings had no obvious effect on the RPR. Conclusion: 3 times firing or more may decrease the bond strength of PFM.
RESUMEN
Objective: To measure the convergence angles of teeth in different teeth positions and different denture designs.Methods:Mesio-distal widths and labio-lingual widths were measured on 226 teeth according to three points in a line on three surfaces (gingival, middle and incise) of each tooth.The distance between every point and the counterpart point in the adjacent surface was measured.Then convergence angles were calculated. Results:The means of the angles were 4.13°±0.77°,4.14°±0.76°, 4.46°±0.95°, 4.76°±0.84° on the first and second premolar, the first and second molar,respectively . There were significant differences between the premolars and molars(P<0.05).On the dentures with the designs of monocrown, triunit-bridge and long bridge the means of the angles were 3.78°±0.74°,4.69°±0.75° and 5.08°±0.85° respectively,the angle on the denture with the design of monocrown was smaller than that of bridge (P<0.05).Conclusion:The convergence angles are different according to different teeth positions and different denture designs, so they should be adjusted according to the clinical requirements.
RESUMEN
Objective:To study the bone-conductivity and absorbability of nano-hydroxyapatite/collagen(nHAC) composite implanted under the calvarial periosteum in rabbits.Methods:24 nHAC samples and 24 HA samples were prepaired in the shape of round disk with the diameter of 8 mm and thickness of 3 mm.nHAC samples were implanted under calvarial periosteum on the left side and HA samples on the right of 24 rabbits.The bone-conductivity and absorbability of the samples were examined by new bone height measuring and residual implant materia measuring 2,4,8 and 12 weeks after operation. Results:2,4,8 and 12 weeks after implantation new bone height(mm) in nHAC group was 0.54?0.09,0.72?0.12,1.83?0.14 and 2.63?0.07,that in HA group 0.13?0.11,0.31?0.12,1.23?0.05 and 1.75?0.14,respectively(P