RESUMEN
OBJECTIVE@#To study the effects of epigallocatechin-3-gallate (EGCG) on proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cell line and analyze the expression of Bcl-2, Bax and Caspase-3 in the cell line which treated with EGCG.@*METHOD@#MTT assay and flow cytometry were used to analyze cell proliferation and cell cycle. Hoechst33258 fluorescence staining was adopted to study cell apoptosis. RT-PCR was used to detect the expression of Bcl-2, Bax, Caspase-3.@*RESULT@#EGCG could significantly inhibit proliferation of CNE-2 cell line and induce its apoptosis with dose-independent relationship. EGCG could suppress the expression of Bcl-2 and induce expression of Bax, Caspase-3.@*CONCLUSION@#EGCG in vitro has efficacy of anti-nasopharyngeal carcinoma cells, which may be through regulating the expression of cell proliferation and apoptosis genes involved.
Asunto(s)
Humanos , Apoptosis , Carcinoma , Caspasa 3 , Metabolismo , Catequina , Farmacología , Línea Celular Tumoral , Proliferación Celular , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas , Metabolismo , Patología , Proteínas Proto-Oncogénicas c-bcl-2 , Metabolismo , Proteína X Asociada a bcl-2 , MetabolismoRESUMEN
Objective To investigate the effects of combination of limited shunt and devascularization in the treatment of patients with portal hypertension.Methods The follow-up results of 39 portal hypertension(patients) undergoing the combined operation were retrospectively analysed.Results The postoperative free(portal) pressure decreased by 8cm H_2O,and hepatopetal portal venous blood flow was maintained.All the(patients) were followed-up for 6 to 24 months,the results showed that various degreas of recovery of liver function in 18 patients.One patient had transient hepatic encephalopathy.Ascites disappeared in 38 patients and gastroesophageal varices improved in 82.1% of patients.No case of rebleeding occured.Conclusions Limited shunt combined with devascularization can effectively maintain the blood supply to the liver,prevent upper gastroesophageal hemorrhage and reduce the occurrence rate of hepatic encephalopathy.It is a suitable operative method for the treatment of portal hypertensive patients.