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1.
Braz. j. med. biol. res ; 53(5): e9608, 2020. graf
Artículo en Inglés | LILACS | ID: biblio-1098119

RESUMEN

Cataract, an eye disease that threatens the health of millions of people, brings about severe economic burden for patients and society. MicroRNA (miR)-378a-5p and miR-630 were recognized as essential regulators in multiple cancers. However, the exact functions of miR-378a-5p and miR-630 in cataract are still unclear. The expression of miR-378a-5p, miR-630, and E2F transcription factor 3 (E2F3) in tissues and cells was measured by quantitative real-time polymerase chain reaction. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay was used to evaluate cell viability. Flow cytometry was conducted to analyze cell apoptosis. The interaction between E2F3 and miR-378a-5p or miR-630 was confirmed by dual-luciferase reporter assay. The expression of proteins E2F3, B cell lymphoma (Bcl-2), Bcl-2 associated X (Bax), and cleaved caspase 3 was detected by western blot assay. The expression of miR-378a-5p and miR-630 was up-regulated whereas E2F3 was down-regulated in human cataract lens tissues compared with normal lens tissues. Depletion of miR-378a-5p or miR-630 enhanced proliferation and reduced apoptosis of human lens epithelial cells. Interestingly, up-regulation of E2F3 exhibited the same trend. Next, dual-luciferase reporter assay validated the interaction between E2F3 and miR-378a-5p or miR-630. The rescue experiments further revealed that E2F3 knockdown could recover miR-378a-5p, and miR-630 inhibitor induced promotion of cell proliferation and inhibition of apoptosis in cataract. miR-378a-5p and miR-630 repressed proliferation and induced apoptosis of lens epithelial cells by targeting E2F3 in cataract, representing a prospective alternative therapy for cataract.


Asunto(s)
Humanos , Masculino , Femenino , Persona de Mediana Edad , Anciano , Catarata/metabolismo , Apoptosis , MicroARNs/metabolismo , Células Epiteliales/metabolismo , Factor de Transcripción E2F3/metabolismo , Regulación hacia Abajo , Western Blotting , Progresión de la Enfermedad , Reacción en Cadena en Tiempo Real de la Polimerasa , Citometría de Flujo
2.
Journal of Prevention and Treatment for Stomatological Diseases ; (12): 405-408, 2017.
Artículo en Chino | WPRIM | ID: wpr-822336

RESUMEN

@#In the process of children’s dental treatment, children’s non-cooperation has always troubled dentists in clinical work. Using behavior management techniques properly can help us manage children’s behavior more effectively, improve the treatment efficiency, and greatly relieve the work stress. This paper reviewed the research progress on the basic and advanced behavior techniques for children’s oral behavior management techniques.

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