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ABSTRACT We previously revealed the involvement of extracellular regulated protein kinases 1/2 (ERK1/2) in interleukin-6 (IL-6) secretion induced by cyclic compressive force (CCF) in MLO-Y4 cells. In this study, we investigated the contributions of the p38 mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways to IL-6 secretion by stimulating MLO-Y4 cells with CCF. At 80% confluence, different magnitudes (1000μstrain, 2000 μstrain and 4000 μstrain), frequencies (0.5 Hz, 1.0 Hz and 2.0 Hz) and durations (10 min, 30 min, 1 h, 3 h and 6 h) of CCF were loaded onto cells using a four-point bending system. Flow Cytometry (FCM) analysis was used to analyze cell mortality rates after CCF loading. p38 and p65 phosphorylation as well as IκBα degradation in MLO-Y4 cells were detected by Western blotting (WB). Changes in IL-6 secretion after inhibitor treatment were assessed by enzyme-linked immunosorbent assays (ELISAs). Cellular viability was over 90 percent after CCF. p38 and p65 phosphorylation increased under all conditions, whereas IκBα protein levels decreased. However, phosphorylation and degradation were not completely dependent on the loading magnitude, frequency or duration. Furthermore, p38 inhibition using the specific inhibitor SB203580 reduced both p38 phosphorylation and IL-6 secretion. Similarly, NF-κB inhibition using BAY 11-7082 decreased p65 phosphorylation and IL-6 secretion but increased the concentration of IκBα. These findings reveal significant roles for the p38 and NF-κB signaling pathways in IL-6 secretion induced by CCF in MLO-Y4 cells.
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Aims: This cross-sectional study aimed to explore potential factors influencing oral health related quality of life (OHRQoL) in a Chinese population treated with anterior dentition fixed dental prosthesis (FDP). Study Design: Cross-sectional study. Place and Duration of Study: West China Hospital of Stomatology, Sichuan University, between December 2012 and May 2013. Methodology: According to the patient records in the dental hospitals, 1918 patients were initially selected and invited for a follow-up examination. After the selection, 987 participants were finally included and asked to complete a questionnaire which included three forms: Basic information, the Chinese version of Oral Health Impact Profile-14 items (OHIP-14) and Social Readjustment Rating Scale (SRRS). After data collection, independent sample t-test, one-way ANOVA and spearman rank correlation were used to assess each variable’s independent impact on OHRQoL, while multifactor impact was evaluated by stepwise linear regression. Results: 987 responses were acquired. Higher age caused a higher OHIP-14 score. For prosthesis material, all ceramic presented the lowest OHIP-14 score. For prosthesis type, veneer presented the lowest OHIP-14 score. Either prosthesis service time or SRRS score was positively correlated to OHIP-14 score. In stepwise linear regression, prosthesis material/service time/type and life stress were finally defined as OHRQoL predictors. Conclusion: Three prosthetic characteristics (prosthesis material/service time/type) and life stress are responsible for OHRQoL level of patients treated with anterior FDP.