RESUMEN
<p><b>OBJECTIVE</b>To investigate the chemical constituents of Corydalis yanhusuo.</p><p><b>METHOD</b>The compounds were isolated and purified by column chromatography over macroporous absorption resin, silica gel, and Sephadex LH-20. Their structures were elucidated on the basis of physicochemical properties and spectral data.</p><p><b>RESULT</b>22 compounds were isolated and identified as corydaline (1), tetrahydropalmatine (2), protopine (3), tetrahydrocorysamine (4), tetrahydrocoptisine (5) , tetrahydroberberine (6), tetrahydrocolumbamine (7), noroxyhydrastine (8), dehydrocorydaline (9), glaucine (10), columbamine (11), 8-oxocoptisine (12), 13-methyl-columbamine (13), coptisine (14), palmatine (15), herberine (16), oxoglaucine (17), 13-methyl-palmatrubine (18), dehydrocorybulbine (19), stepharanine (20), adenosine (21), and N5 -acetylornithine (22).</p><p><b>CONCLUSION</b>Compounds 13, 20, 21, and 22 were isolated from this plant for the first time.</p>
Asunto(s)
Adenosina , Alcaloides , Apomorfina , Aporfinas , Berberina , Alcaloides de Berberina , Cromatografía Liquida , Métodos , Corydalis , Química , Extractos Vegetales , Espectrometría de Masa por Ionización de Electrospray , MétodosRESUMEN
<p><b>OBJECTIVE</b>To investigate the chemical constituents of the branches and leaves of Polyalthia nemoralis.</p><p><b>METHOD</b>The compounds were isolated and purified by silica gel, macroporous adsorption resin and Sephadex LH-20 column chromatographic methods. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.</p><p><b>RESULT</b>Fourteen compounds were isolated and identified as syringic acid (1), 3-methoxy-4-hydroxycinnamic acid (2), vanillic acid (3), 4-hydroxybenzoic acid (4), mauritianin (5), (+)-xylopinidine (6), (+)-oblongine(7), (+)-tembetarine (8), eythritol (9), D-mannitol (10), ethyl-beta-D-glucopyranoside (11), (+)-magnoflorine (12), stepharanine (13), (2S, 4R)-4-hydroxy-2-piperidine-carboxylic acid (14), respectively.</p><p><b>CONCLUSION</b>All the compounds were isolated from the genus Polyalthia for the first time; compounds 6 and 13 showed inhibitation activities against multi tumor cell lines.</p>
Asunto(s)
Humanos , Alcaloides , Química , Farmacología , Antineoplásicos Fitogénicos , Química , Farmacología , Aporfinas , Química , Farmacología , Línea Celular Tumoral , Supervivencia Celular , Cromatografía en Agarosa , Métodos , Ácidos Cumáricos , Química , Farmacología , Ácido Gálico , Química , Farmacología , Quempferoles , Química , Farmacología , Parabenos , Química , Farmacología , Extractos Vegetales , Química , Farmacología , Hojas de la Planta , Química , Tallos de la Planta , Química , Polyalthia , Química , Ácido Vanílico , Química , FarmacologíaRESUMEN
<p><b>OBJECTIVE</b>To investigate the active constituents of the branches and leaves of Polyalthia nemoralis.</p><p><b>METHOD</b>The compounds were isolated and purified by silica gel, macroporous adsorption resin and Sephadex LH-20 column chromatographic methods. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.</p><p><b>RESULT</b>Eight compounds were isolated and identified as: zincpolyanemin (1), nickel bis-(pyridine-N-oxide-2-thiolate) (2), cupric bis (pyridine-N-oxide-2-thiolate (3), 2-methanesulfonyl-pyridine (4), 2-pyridinethiolate N-oxide (5), 2,2'-dithiodipyridine (6), 2-thiohydroxypyridin-N-oxide-2-S-beta-D-gluco pyranoside (7) and pyridine-N-oxide (8), respectively.</p><p><b>CONCLUSION</b>Compounds 2, 4-6, 8 were new natural products. The bioassays in vitro against five human tumor cell lines with MTT method showed stronger cytotoxic activities (IC50 0.05-0.09 mg x L(-1)) for compounds 1-3 and 6, and weaker cytotoxic activities (IC50 5.49-7.71 mg x L(-1)) for compound 5.</p>
Asunto(s)
Humanos , Línea Celular Tumoral , Supervivencia Celular , Medicamentos Herbarios Chinos , Química , Farmacología , Espectroscopía de Resonancia Magnética , Hojas de la Planta , Química , Tallos de la Planta , Química , Polyalthia , Química , Piridinas , QuímicaRESUMEN
<p><b>OBJECTIVE</b>To investigate the aporphine alkaloids in the branches and leaves of Polyalthia nemoralis.</p><p><b>METHOD</b>The compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatographies. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.</p><p><b>RESULT</b>Five aporphine alkaloids were isolated and identified as: bidebiline A (1), annobraine (2), lanuginosine (3), liriodenine (4), oxostephanosine (5), respectively.</p><p><b>CONCLUSION</b>For the first time, Compounds 2 and 5 were obtained from Polyalthia while 1, 3 and 4 isolated from this plant. The bioassays in vitro against five human tumor cell lines with MTT method showed moderate cytotoxic activities (IC50 1 mg x L(-1)) of compounds 3-5.</p>