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OBJECTIVE To explore the mechanism of oridon in（Ori）reversing the drug resistance of breast cancer cell MCF-7 to fulvestrant （Ful）. METHODS Ful-resistant breast cancer cell strains MCF- 7/Ful were induced and constructed in vitro . The relative cell viability of MCF- 7 cells and MCF- 7/Ful cells was detected by MTT assay ，inhibitory rate of Ori to MCF- 7/Ful cells was also detected. CompuSyn software was used to analyze the synergistic effect of Ori and Ful. MCF- 7/Ful cells were randomly divided into blank control group ，Ful group （5 μmol/L），Ori group （8 μmol/L），Ful（5 μmol/L）+Ori（8 μmol/L）group. The phosphorylation of phosphatidylinositol 3 kinase（PI3K）/protein kinase B （Akt）signaling pathway related protein in each group was detected by Western blot. MCF-7/Ful cells were used to prepare drug resistance model of transplanted tumor in nude mice，and they were randomly divided into blank control group ，Ful group （80 μmol/g），Ori group （50 μmol/g），Ful（80 μmol/g）+ Ori（50 μmol/g）group. The tumor weight and tumor inhibition rate were calculated ，to verify the reversal effect of Ori and Ful in vivo. RESULTS MTT assay showed that when Ful ≥10 μmol/L，the relative cell viability of MCF- 7/Ful cells was significantly higher than that of MCF- 7 cells（P＜0.05），and the drug resistance was significantly enhanced ；Ori had a significant inhibitory effect on MCF- 7/Ful cells ，the inhibition rate of Ori combined with Ful to MCF- 7/Ful cells was significantly increased （P＜0.05 or P＜0.01），and the effect of reversing drug resistance was significantly increased . The results of Western blot showed that compared with Ful group ，the phosphorylation levels of PI 3K and Akt protein in Ful+Ori group were significantly decreased （P＜0.05 or P＜ 0.01）. In vivo results showed that the tumor volume and mass of Ful+Ori group were significantly decreased ，and the tumor inhibition rate was （63.90±4.11）％，which was significantly higher than that of Ful group （P＜0.01）. CONCLUSIONS Ori can reverse drug resistance of breast cancer cell MCF- 7 to Ful ， and this effect may be through regulating PI 3K/Akt signaling pathway.
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AIM:To evaluate the clinical effect of Fuzheng Jiedu Granule(Radix Hedysari,Poria,Radix Angelicae sinensis,Fructus Forsythiae,Rhizoma Curcumae,Radix et Rhizoma Salviae miltiorrhizae,etc.) on patients with coronary heart disease who have longterm contact with nickel,and observe its influence on the level of Creatine kinase(CK),Lactate dehydrogenase(LDH),Na~+-K~+-ATPase,Ca~(2+)-ATPase in RBC membrane,Nitric oxide(NO),Nitric oxide synthase(NOS),Nickel(Ni) in serum. METHODS: 60 cases were divided into two groups A and B randomly.30 cases in group A were treated with Fuzheng Jiedu Granule combined with western medicine Betaloc and Isosorbide Dinirrate;30 cases in group B were given only western medicine.The period of treatment was 4 weeks.Another 15 healthy cases selected as group C(healthy control group).Before and after treatment,the clinical effects were observed,meanwhile,some biochemical indexes such as CK,LDH,Na~+-K~+-ATPase,Ca~(2+)-ATPase in RBC membrane,NO,NOS,Ni in serum were measured with clinic test methods.Finally,the experimental results were compared and analyzed in the three groups mentioned above. RESULTS: The therapeutic effect of symptoms and signs of group A was superior to that of group B(P