RESUMEN
Background: Polycystic ovary syndrome [PCOS] is a common but complex endocrine disorder and is the major cause of anovulation and consequent subfertility. In this study the effect of grape seed extract [GSE] on triglyceride [TG], total cholesterol [TC], high-density lipoprotein-cholestrol [HDL-C], low-density lipoprotein-cholestrol [LDL-C] and interleukin-6 [IL-6] in PCOS Wistar rats were assessed
Materials and Methods: In this experimental study, 84 adult female Wistar rats were divided into 7 groups [n=12] including control [intact], Sham [estradiol valerate solvent injection], control PCOS and 4 experimental PCOS groups. To induce the syndrome, a single subcutaneous injection of 2 mg estradiol valerate was applied. In experimental groups, PCOS rats were treated with different doses of 50, 75, 100 and 200 mg/kg body weight [BW] GSE by intraperitoneal injection for 10 consecutive days. After harvesting blood serum, TG was measured by Glycerol-3-phosphate Oxidase-Peoxidase [GPO-PAP], TC by Cholesterol Oxidase-Peroxidase [CHOD-PAP], and HDL-C by sedimentation method, LDL-C by Friedwald calculation and IL-6 by ELISA method. The serum values of each parameter were analyzed using one-way ANOVA at P<0.05
Results: In all experimental groups significant decrease of visceral fat was obvious as compared with control PCOS group. LDL-C, TC and IL-6 levels in experimental groups, particularly at dose of 50 mg/kg of GSE, were significantly decreased as compared with PCOS group. However, HDL-C levels were not significantly changed
Conclusion: : According to the findings of this study, it can be concluded that GSE with its effects on serum TC, LDL-C and IL-6 could reduce the effects of dyslipidemia and inflammation in PCOS rats and improve systemic symptoms of PCOS