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Chinese Journal of Biotechnology ; (12): 1107-1111, 2007.
Artículo en Chino | WPRIM | ID: wpr-276153

RESUMEN

According to published nucleotide sequences, ORF4 gene of barley yellow dwarf virus GAV (BYDV-GAV) was synthesized by reverse transcription-polymerase chain reaction (RT-PCR). The BYDV-GAV ORF4 gene was expressed in baculovirus -insect cell expression system efficiently, and western bolt analysis confirmed its expression product. Confocal laser scanning microscopy showed that GFP: ORF4 fusion protein was associated with the nuclear envelope of insect cells. By expressing the N- and C-terminal regions of ORF4-encoding product (P4) in insect cells combined with structure prediction, it was found that the N-terminal region of P4 containing four a-helices is required for targeting P4 to the nuclear envelope. These results provide a base for biological function of ORF4 gene during systemic infection of BYDV-GAV in host plants further.


Asunto(s)
Animales , Secuencia de Aminoácidos , Baculoviridae , Genética , Metabolismo , Genes de Plantas , Genética , Proteínas Fluorescentes Verdes , Genética , Insectos , Genética , Metabolismo , Luteovirus , Genética , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Genética , Proteínas Recombinantes de Fusión , Genética , Metabolismo
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