1.
Gezira Journal of Health Sciences. 2007; 3 (1): 29-41
en Inglés
| IMEMR
| ID: emr-82531
RESUMEN
The parotoid gland secretion of Bufo spp., obtained by manual compression, was found to contain 23.1-41.2% [w/w] of total protein. Gel filtration chromatography showed the existence of four fractions of peptides and proteins responsible for antibacterial activity. Thin layer chromatography showed seven Ninhydrin-positive spots in addition to the origin, in the parotoid gland secretion of Bufo spp. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis revealed the existence, in the crude secretion, of 2 bands of proteins [P[1] and P[2]] and 2 bands of peptides [P[3] and P[4]] with various molecular weights