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Aim To investigate the effect of astragalo- side Ⅳ on focal cerebral ischemia-reperfusion injury in rats.Methods The focal cerebral ischemia/reper-fusion of rat left middle cerebral artery occlusion ( MCAO) was induced by suture method .Male SD rats were randomly divided into sham operation group , cer-ebral ischemia/reperfusion group , astragaloside IV group and solvent control group .Except for the sham operation group , the others were subjected to ischemia 2h and reperfusion 24h.Then, rats with successful model were chosen for the detection of various indexes . Astragaloside IV group was injected intraperitoneally with astragaloside IV(20 mg· kg -1 ) at the same time as reperfusion , while solvent control group was injected with the same amount of solvent .TTC staining was used to detect the volume of cerebral infarction , and Nissl staining to observe the changes of histomorpholo-gy, and transmission electron microscope (TEM) to ob-serve the ultrastructure of the cells .Results There was no neurological deficit in the sham operation group, and the volume of cerebral infarction was zero . Compared with the sham operation group , there were some increased neurological deficits , nerve cell damage and cerebral infarction volume in other groups ( P <0.05) .Compared with the cerebral ischemia/reperfu-sion group , the nerve function damage could be signifi-cantly improved , the damage of neurons reduced , and the volume of cerebral infarction decreased ( P<0.05 ) in astragaloside IV group , and there was no obvious change in the solvent control group ( P>0.05 ) .Con-clusion Astragaloside IV can reduce the focal ische-mia/reperfusion injury in rats and protect nerve cells from damage.
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Objective: To investigate the effect of moxibustion at Shenque (CV 8) on fatigue in rats with chronic exercise-induced exhaustion. Methods: Thirty male Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group and a moxibustion group, 10 rats in each group. Except rats in the blank group, the remaining rats were subjected to create long-term exhaustion models by repeated swimming. After successful modeling, rats in the moxibustion group received mild moxibustion at Shenque (CV 8) for 15 min, once every other day with a total of 10 times. Rats in the model group and the blank group did not receive moxibustion. At the end of the treatment, the exhausted times, and the body weight of rats before and after the experiment were compared among groups. The levels of blood malondialdehyde (MDA) and urea nitrogen (BUN), as well as the activities of aspartate transarninase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were also measured by the automatic biochemical analyzer, 24 h after the exhausting excise. Results: The 10th swimming time was significantly longer in the moxibustion group than that in the model group (P<0.01). The increase rate of the body weight was lower in the rats of the moxibustion group than that in the model group before the 7th and the 10th exhausting excise (P<0.05, P<0.01). The levels of serum MDA and BUN, as well as the activities of AST, ALT and LDH in the model group were higher than those in the blank group (all P<0.01). The levels of serum MDA and BUN, as well as the activities of AST, ALT and LDH in the moxibustion group were lower than those in the model group (P<0.01). Conclusion: Moxibustion at Shenque (CV 8) can decrease the serum levels of MDA and BUN, as well as activities of AST, ALT and LDH in the long-term fatigue rats, thus to improve the symptoms of fatigue.
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Objective: To observe the difference of the effect of low-frequency electroacupuncture (EA) on blood lipids between male and female patients with simple obesity due to damp induced by spleen deficiency. Methods: Eighty patients with simple obesity were recruited, including 37 males and 43 females, to receive low-frequency EA by selecting Yinlingquan (SP 10), Sanyinjiao (SP 6), Zusanli (ST 36), Fenglong (ST 40), Quchi (LI 11), Tianshu (ST 25), Zhongwan (CV 12), Shuifen (CV 9), Qihai (CV 6) and Guanyuan (CV 4), with needles retained for 30 min. The treatment was given once a day, 10 sessions as a treatment course, for 2 courses in total. The contents of body fat percentage (F%), total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), blood glucose (Glu) and adiponectin (ADPN) in serum were observed to see the changes, and the two groups were compared and analyzed. Results: After the treatment, F%, and serum contents of TC, TG, LDL, Glu and ADPN dropped significantly in the two groups (P<0.05 or P<0.01) and the serum content of HDL increased significantly in male group (P<0.05). The decrease of F% in female group was more significant than that in male group (P<0.01); the decrease of ADPN in male group was more significant than that in female group (P<0.05). Conclusion: EA can regulate the disordered blood lipids in male and female patients with simple obesity, with certain differences between genders. The decrease of subcutaneous fat content is more significant in females than that in males, while the decrease of ADPN is more significant in males.