RESUMEN
Objective To isolate endophytic fungi from the fresh leaves of Dioscorea nipponica Makino,and to determine their antimicrobial activities.Methods Endophytic fungi from the fresh leaves of Dioscorea nipponica Makino were isolated by tissue isolation method,and the preliminary identification was carried out by slide plate culture,tissue culture,and plug culture.After isolating the endophytic fungi,the antimicrobial activity from the isolated endophytic fungi was detected using filter paper method with Staphylococcus aureus,Staphylococcus albus,Staphylococcus citreu,Micrococcus tetragenus,Escherichia coli,Salmonella typhi,Pseudomonas aeruginosa,Bacterium termo,Bacillus endocarditidiscapsulatus,Shigella flexneri,Bacterium paratyphosum B indicator microorganisms.Results A total of 52 strains of the endophytic fungi were isolated.According to morphological characteristics,10 endophytic fungi producing spores were identified as two orders,three families,and nine genera.The extracts of fungal mycelium had the antibiotic activity of strains of C37,C30,and C50.With the identification by morphology and molecular biology,strain C37 was determined to belong to the genus Gibberella,and C30,C50 to Alternaria.Conclusion The antibacterial activity of these endophytes in the leaves ofDioscorea nipponica Makino could be exploited for the development of new antibacterial biological agents.