Quantitative study of porphyromonas gingivalis in subgingival plaques of orthodontic adults / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To assess the early variation of clinical periodontal indexes and Porphyronwnas gingivulis (Pgingivalis) in subgingival plaques of orthodontic adults.</p><p><b>METHODS</b>11 orthodontic adults were selected. Clinical periodontal indexes of observed teeth were examined at three different time points: Before orthodontic treatment, the first month after treatment and the third month after treatment, and subgingival plaques were collected simultaneously at each time point. Clinical periodontal indexes included four ones: Plaque index (PU), sulcus bleeding index (SBI), probing depth (PD), and attachment loss. We used real-time quantitative polymerase chain reaction to detect and quantitate the number of total bacteria and P.gingivalis in each sample to obtain positive rate of P.gingivalis and the percentage of P. gingiaalis in total bacteria.</p><p><b>RESULTS</b>PLI and SBI of the first and third month were more than that of the baseline (P < 0.05). PD rose at the first month after treatment (P < 0.05), and then dropped at the third month. PD of all the 11 participants was lower than 2 mm. No attachment loss was found. The positive rate of P.gingivolis was stable (45.5%) and the proportion of Pgingivalis had no significant difference (p > 0.05) at each time point.</p><p><b>CONCLUSION</b>Fixed orthodontic appliances caused plaque accumulation, accordingly slight gingiva inflammation and the increasement of P.gingivalis occurred in the early stage, hut none periodontitis was found.</p>

Epidemiological study on rotavirus-borne diarrhea in infants and children in different areas / 中华流行病学杂志

<p><b>OBJECTIVE</b>To analyze and compare the epidemiological features of rotavirus diarrhea among infants in the different areas so as to provide data for rotavirus vaccine research.</p><p><b>METHODS</b>From Sep. 2001 through Sep. 2003, sentinel sites were set up in Suzhou Children's Hospital and Maanshan Hospital. Fecal samples from children (< 5 years) with acute diarrheal were collected and enzyme linked immunosorbent assay was used to detect rotavirus antigen. Reverse transcription-polymerase chain reaction was used to determine the G serotypes and P genotypes of rotavirus strains. The features of strains in the two places and other areas of China were analyzed and compared.</p><p><b>RESULTS</b>(1) Rotavirus infection appeared in autumn and winter, but the peaks varied. In Suzhou the peaks were from December to next February in 2001, and November to next January in 2002. But in Maanshan, it was November to next January for both two years. (2) Rate of rotavirus infection in Suzhou was much higher than that in Maanshan, infective rates of Inpatient Department and Outpatient Department are 47.28%, 28.39% and 30.38%, 14.77% respectively in the two hospitals. (3) Rates of infection in two hospitals showed age difference but the highest group was in 6 - 35 month-olds. No gender difference was found. (4) Secular distribution of G-typing and P-typing of rotavirus strain was different in Suzhou and Maanshan. G3 was mainly found in Suzhou and G1 in Maanshan. From 2002-2003 on, G3 became dominant in Maanshan.</p><p><b>CONCLUSION</b>Rotavirus caused diarrhea among infant and children were different in terms of areas, period and types, suggesting that the introduction of rotavirus vaccine should be adjusted according to different strains with specific types and optimal timeline.</p>

Study on Clinical Features of Rotavirus Diarrhea and G Type Serum in Infants and Children in Maanshan and Suzhou Areas / 实用儿科临床杂志

Objective To investigate the clinical features and distribution of G types of rotavirus diarrhea in infants and children in Suzhou and Maanshan areas.Methods 1267 stool specimens were collected from children under 5 years with acute diarrhea. Enzyme-linked immunosorbant assay (ELISA) and nested polymeras chain reaction (Nested-PCR) were used to detect rotavirus and serotypes.Results In 1267 stool specimens, 378 were detected RV positive, and serotype G was the prevalent type in the two cities with number of 250 and the G3 and G1 types were the most prevalent which accounted for 40%(100/250) and 44.40 % (111/250), respectively. While the G2 ,G4, G9 types and mixed infections were 8,3,3,4 and the un-identified type was 8.40%(21/250). G1 type was the most prevalent in Maanshan are which accounted for 58.54% in all cases,while the G3 type accounted for the most of case(47.85%) in Suzhou area.Conclusions The results indicated that rotavirus was the most important etiologic agent in Maanshan and Suzhou areas,G1 and G3 were the most prevalent types,respectively. J Appl Clin Pediatr,2005,20(3):208-210