RESUMEN
Objective · To study the effect of miR-137 overexpressed trophoblast cells on the biological functions of vascular endothelial cells.Methods · Lentivirus vectors Up-LV-miR-137 and LV-miR-NC were constructed and transfected into the trophoblast cells HTR-8/SVneo, respectively.And transfection efficiency was verified by PCR. Vascular endothelial cells were cultured with transfected trophoblast cells or their culture medium supernatant. Proliferation, migration activity and ability to recruit monocytes U937 of endothelial cells were detected by CCK8 method, scratch test and Transwell migration test, respectively. Results · miR-137 overexpressed trophoblast cells were obtained. The results of CCK8 test showed that proliferation of vascular endothelial cells cultured with miR-137 overexpressed trophoblast cells or their culture medium supernatant was inhibited, especially in high glucose condition. The repair of endothelial cells in the transfected cells culture medium slowed down in the scratch test. Transwell migration test results showed that after the endothelial cells were cultured with miR-137 overexpressed trophoblast cells, more monocytes passed through the chambers with higher chemotactic index. Conclusion · miR-137 overexpressed trophoblast cells can regulate the biological functions of vascular endothelial cells, i.e. reduce proliferation and migration activity and enhance ability to recruit monocytes.
RESUMEN
Objective · To study the effect of miR-137 overexpressed trophoblast cells on the biological functions of vascular endothelial cells.Methods · Lentivirus vectors Up-LV-miR-137 and LV-miR-NC were constructed and transfected into the trophoblast cells HTR-8/SVneo, respectively.And transfection efficiency was verified by PCR. Vascular endothelial cells were cultured with transfected trophoblast cells or their culture medium supernatant. Proliferation, migration activity and ability to recruit monocytes U937 of endothelial cells were detected by CCK8 method, scratch test and Transwell migration test, respectively. Results · miR-137 overexpressed trophoblast cells were obtained. The results of CCK8 test showed that proliferation of vascular endothelial cells cultured with miR-137 overexpressed trophoblast cells or their culture medium supernatant was inhibited, especially in high glucose condition. The repair of endothelial cells in the transfected cells culture medium slowed down in the scratch test. Transwell migration test results showed that after the endothelial cells were cultured with miR-137 overexpressed trophoblast cells, more monocytes passed through the chambers with higher chemotactic index. Conclusion · miR-137 overexpressed trophoblast cells can regulate the biological functions of vascular endothelial cells, i.e. reduce proliferation and migration activity and enhance ability to recruit monocytes.