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AIM To study the therapeutic effect of Moslae Herba volatile oil on rat models with the spleen and the stomach stranded by dampness,and to form the modern pharmacology basis in terms of Moslae Herba's power in transforming dampness and harmonizing the center.METHODS The rat models of dampness-trapped spleen and stomach were induced by improper diet,damp environment and forced swimming.The model rats,ten in each group,were then randomly divided into model group,groups of low-dose,medium-dose and high-dose volatile oils,Twain-80 group,and positive drug group.The medication impact on rat models intragastrically administered for fif-teen days were assessed from body weight,food intake,water intake,urine,stool weight,spleen and thymus organ coefficient and gastric emptying and small intestinal propulsion rate and intestinal moisture index and serum motilin of rats (MTL) and gastrin (GAS),Na+-K +-ATP enzyme,amylase (AMS),interleukin-6 (IL-6),immunoglobulin G (IgG),gastrointestinal aquaporin 2 (AQP2),and indexes of pathological tissue change.RESULTS Compared with the model group,rats in each medication group displayed overall improvement in body weight,food intake,water intake,urine volume,spleen and thymus organ coefficient,gastric emptying and small intestinal propulsion rate,wet stool weight and intestinal water content,serum Na +-K +-ATP,GAS,AMS enzyme activity,IgG content,IL-6,MTL,AQP2 content of digestive tract,and the differences were statistically significant.CONCLUSION Moslae Herba works through its gastrointestinal absorption,and the ability in regulating water metabolism and immunity,that is to improve functioning of the spleen and the stomach in resolving dampness and harmonizing the center.
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Objective To provide the feasible basis for the implementation of integration processing technology of origin of Moslae Herba by comparing the traditional cutting processing and integration processing of origin of Moslae Herba. Methods Taking the softening time, the length of cutting section and the drying temperature as the investigation factors of the traditional cutting processing, and the cutting length, drying temperature and drying time as the investigation factors of the integration processing of origin, L9(34) orthogonal test was used. Carvacrol, thymol, volatile oil content were setting as the evaluation indexes, the traditional cutting processing and the integration processing of origin were optimized, and the two selected process were compared. The contents of carvacrol and thymol were deterrmined by Agilent 1200 high performance liquid chromatography and ECOSIL-C18 column. The mobile phase was acetonitrile-0.2％ acetic acid, gradient elution, with wavelength of 274 nm, flow rate of 1.0 mL/min, sample volume of 20 μL. The extraction of the volatile oil was in accordance with the volatile oil determination method in Chinese Pharmacopoeia 2015 edition. Results The optimized traditional cutting processing: softening 1 h with 3 times amount of water, cutting to 1.0 cm, drying at 50–60 ℃. The optimized integration processing of origin: cutting to 1.0 cm in fresh, drying at 50–60 ℃, drying to 36 h. The contents of carvacrol, thymol, and volatile oil in the optimum integration processing of origin were all higher than the traditional cutting processing. Conclusion The technology of integration processing of origin is simple and convenient, which can make up the defects of the complicated traditional cutting processing and the lessen of effective components, and is feasible.
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OBJECTIVE@#To investigate the role of T help 17 cells (Th17) and STAT3-VEGF pathway in pathogenesis of psoriasis.@*METHODS@#A total of 50 cases of psoriasis guinea pigs and 20 normal guinea pigs were selected. The ratio of Th17/ IL-17 cell in peripheral blood were detected by flow cytometric analysis; STAT3 and VEGF concentrations were measured by immunohistochemistry and Western blot.@*RESULTS@#The expression of Th17 in peripheral blood were significantly increased in psoriasis [(1.76±0.88)%] compared with controls [(0.48±0.27)%] (P<0.05). Th17 related cytokine STAT3 and VEGF were significantly increased in psoriasis compared with controls (P<0.05), and were positively correlated the expression of Th17.@*CONCLUSIONS@#The expressions of Th17, STAT3 and VEGF are elevated in psoriasis, which suggests Th17 cells have a potential role in the pathogenesis of psoriasis by STAT3-VEGF pathway.