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Objective To study the situation of pathogenic bacteria of neonate infection in hospital,so can guide clinical doctors use antibiotic rationally. Method The secretions of 159 neonates′ umbilous and eye matter orders were collected to be used as the specimen and the drug susceptibility experiment was done using MIC and K-B methods together in 159 neonates with hospital infection.Results One hundred and eighty-nine pathogenic bacterias were isolated from 1613 specimens.According to our materials,staphylococci aureus was the most important pathogenic bacteria,then staphylococci heamolyticus,staphylococci epidermidis,encherichia coli ,enterobater cloucac ,klebsiella pneumoniae.MRSA- positive rate was 52.6%,ESBLs-positive in E coli was 21%,inklebsiella pneumoniae was 20%.Drugs of sensitivity for Gram positive coccus were vancomycin(0) clindamycin(8.5%) cipnofloxacin(12.2%);the drugs of sensitivity for Gram negative ord were imipenem (4.4%),cipnofloxacin(5.3%),amikacin(12.7%).Conclusion It is instructive that use antibiotic rationally for controlling neonate infection in hospital.
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Objective To investigate the distribution,drug resistance characteristics and genotypes of extended-spectrum-lactamases (ESBLs)-producing strain in pathogenic gram-negative rod in infection of newborn in Guangzhou.Methods The standard was performed by the production for ESBLs by phenotypic screening and confirmatory test provided by the National Committee for Clinical Laboratory Standards in 2001.The method of polymerase chain reaction(PCR) amplification was perbonmed and DNA sequences were analyzed by ESBLs gene sequencing.Results Total of 71 un-repicated and consecutive Gram-negative bacilli were isolated from 13 hospitals in Guangzhou,and the prevalence of ESBLs-producing clinical Gram-negative isolates was 59.2%(42/71).The PCR results showed that most pathogenic bacilli which infected newborn could be separated two or more genes of ESBLs.The type of TEM,SHV,CTX-M1,CTX-M9,OXA was 35.6%, 26.7% ,10.9%,24.8%,2.0%,respectively.The result of drug resistance monitoring showed that pathogenic gram-negative bacillui which infected newborn were Escherichia coli and Klebsiella pneumonia mostly.Most parts of them were drug fast and even multidrug resistant to the commonly used antibiotics.The sensitive drugs were lmipenem(the rate of sensitivty 100%),cefoperazone/sulbactam(87.3%),piperacillin/tazbatam(85.3%),ceftazidime(82%),aztreonam(82%),cefepime(81.8%).Conclusions In Guangzhou,the incidence rate of ESBLs-producing strain are very high inpathogenic bacilli which infected in newborn and is multidrug resistance.The genetypes of produced ESBLs are TEM,SHV,CTX-M1,CTX-M9,OXA.