RESUMEN
<p><b>OBJECTIVE</b>To evaluate the effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and to explore the possible application on the alveolar bone regeneration.</p><p><b>METHODS</b>To determine the optimum concentration, the effects of ginsenoside Rg-1 ranging from 10 to 100 μmol/L were evaluated by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide, alkaline phosphatase activity and calcium deposition. Expressions of runt-related transcription factor 2, collagen alpha-2(I) chain, osteopontin, osteocalcin protein were examined using real-time polymerase chain reaction.</p><p><b>RESULTS</b>Compared with the control group, a certain concentration (10 μmol/L) of the Rg-1 solution significantly enhanced the proliferation and osteogenic differentiation of hPDLSCs (P<0.05). However, concentrations that exceeds 100 μmol/L led to cytotoxicity whereas concentrations below 10 nmol/L showed no significant effect as compared with the control.</p><p><b>CONCLUSION</b>Ginsenoside Rg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10 μmol/L.</p>
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Adolescente , Humanos , Adulto Joven , Fosfatasa Alcalina , Metabolismo , Biomarcadores , Metabolismo , Calcificación Fisiológica , Diferenciación Celular , Proliferación Celular , Separación Celular , Forma de la Célula , Células Cultivadas , Citometría de Flujo , Ginsenósidos , Farmacología , Osteoblastos , Metabolismo , Osteogénesis , Genética , Ligamento Periodontal , Biología Celular , Reacción en Cadena en Tiempo Real de la Polimerasa , Células Madre , Biología Celular , Factores de TiempoRESUMEN
<p><b>OBJECTIVE</b>To observe the effects of berberine hydrochloride on the secretion of monocyte chemoattractant protein-1 (MCP-1) from human periodontal ligament cells (PDLC) in vitro culture.</p><p><b>METHODS</b>Periodontal ligament was isolated from extracted human premolars, and PDLC were cultured in vitro. PDLC were divided into two groups, lipopolysaccharide (LPS) group and non-lipopolysaccharide (NLPS) group. Then in accordance with the final concentrations of berberine hydrochloride in cells culture medium (0, 0.01, 0.02, 0.03 g/L), the groups were subdivided into LPS and NLPS control group, LPS1 and NLPS1 group, LPS2 and NLPS2 group, LPS3 and NLPS3 group. Cellular concentration of MCP-1 of each group was determined by enzyme-linked immunosorbent assay (ELISA). The data were statistically analyzed.</p><p><b>RESULTS</b>The MCP-1 contents were not significantly different between the groups of NLPS1, NLPS2 and NLPS3 [(11.33 ± 0.16), (11.45 ± 0.53), (11.25 ± 0.14) ng/L, respectively] and the NLPS control group [(11.32 ± 0.35) ng/L] (P = 0.692, 0.568, 0.524). MCP-1 contents in the groups of LPS1, LPS2 and LPS3 [respectively (38.14 ± 5.34), (34.15 ± 3.36), (26.13 ± 2.12) ng/L] were significantly lower than in LPS control group [(58.42 ± 1.52) ng/L], P = 0.000, 0.000, P = 0.000.</p><p><b>CONCLUSIONS</b>The inhibitory effect of berberine hydrochloride on the activities of MCP-1 from PDLC was more significant when PDLC were stimulated with LPS and in a concentration-dependent manner.</p>
Asunto(s)
Adolescente , Niño , Humanos , Antiinflamatorios , Farmacología , Berberina , Farmacología , Diente Premolar , Biología Celular , Células Cultivadas , Quimiocina CCL2 , Secreciones Corporales , Relación Dosis-Respuesta a Droga , Lipopolisacáridos , Farmacología , Ligamento Periodontal , Biología Celular , MetabolismoRESUMEN
<p><b>OBJECTIVE</b>To determine the effects of berberine hydrochloride on the expressions of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) in periodontal tissues in rat periodontitis model.</p><p><b>METHODS</b>Based on the successful rat periodontitis model, the experimental rats were randomized into different groups followed by oral treatment with berberine hydrochloride for 1, 2, 3, 4 weeks and then the rats were sacrificed and analyzed. Pathological assay and HE staining were used to detect the general conditions and pathological changes of rat periodontal tissues. And immunohistochemical staining was conducted to determine the expressions of IL-1beta and TNF-alpha in rats periodontitis model periodontal tissues.</p><p><b>RESULTS</b>The levels of IL-1beta and TNF-alpha in the periodontitis tissues were significantly higher than that in the control group. Treatment with berberine hydrochloride decreased the levels of IL-1beta and TNF-alpha in periodontitis tissues (P<0.05). Moreover, the general conditions and pathological changes in the control group and groups treated with berberine hydrochloride were much better than that in periodontitis groups.</p><p><b>CONCLUSION</b>Berberine hydrochloride inhibited the expression of IL-1beta and TNF-alpha in periodontal tissues in rats periodontitis model and promoted the regeneration of the periodontal tissues. This study suggested that berberine hydrochloride may have potential clinical application.</p>
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Animales , Masculino , Ratas , Berberina , Interleucina-1beta , Periodontitis , Factor de Necrosis Tumoral alfaRESUMEN
By confronting the opportunity and challenge of integrative Chinese and Western medicine (ICWM), in this paper, a comprehensive retrospective summary and preliminary discussion was given in aspects of the concept, historical background, acquired fruits, existing problems as well as the developing direction in the future and how to carry out academic researches of ICWM.
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Humanos , Diagnóstico Diferencial , Medicina Tradicional China , Métodos , Proyectos de InvestigaciónRESUMEN
Objective To observe the effects of berberine hydrochloride on the secretion of interleukin-6(IL-6)from human periodontal ligament cells(PDLCs)cultured in vitro.Methods Periodontal ligament tissues were isolated from extracted human premolars,and PDLCs were cultured in vitro.After identification by immunohistochemistry technique,human PDLCs were divided into LPS group and NLPS group according to whether lipopolysaccharide(LPS,50 ?g/mL)was contained in cell culture media.Then in accordance with the final concentrations of berberine hydrochloride in cell culture media(0,0.010,0.020,and 0.030 g/L),the groups were subdivided into LPS and NLPS control group,LPS1 and NLPS1 group,LPS2 and NLPS2 group,LPS3 and NLPS3 group.IL-6 contents in supernatants of each group were determined by enzyme-linked immunosorbent assay,and statistical analysis and comparison were conducted as well.Results Compared with corresponding LPS and NLPS groups,IL-6 contents in supernatants of LPS3 group and NLPS3 group reduced markedly(P