Vaccine pretreatment for quantification of 146S antigen in foot-and-mouth disease vaccines by high performance size exclusion chromatography / 生物工程学报

We developed a pre-treatment method to remove interfering substances during quantification of 146S antigens in foot-and-mouth disease (FMD) vaccines by high performance size exclusion chromatography (HPSEC). Three methods, including ultracentrifugation, PEG precipitation and nuclease digestion, were optimized and compared for removal efficiency of the interfering impurities in FMD vaccines. Under optimized conditions, the 146S contents in two batches of FMD vaccines were determined to be 7.1 and 7.6 μg/mL by ultracentrifugation, 9.7 and 10.4 μg/mL by PEG precipitation, and 10.5 and 10.4 μg/mL by nuclease digestion. The optimal condition for nuclease digestion using Benzonase determined by response surface method was as follows: appending Benzonase into 200 μL of antigen phase to a final concentration of 421 U/mL and incubating at 25.1 °C for 1.29 h. This method has advantages including efficient removal of the interfering impurities, fast processing speed, and mild operating conditions. Then 12 bathes of FMD vaccines with different serotypes produced by 4 manufacturers were tested to verify the established treatment method. Results showed the method was applicable to various FMD vaccines with good reproducibility (RSD<5.3%, n=3). The developed method removed interference from impurities during quantification of 146S, and therefore would broaden the application of HPSEC in vaccine quality control and ensure the accuracy and reliability.

Using size-exclusion chromatography to quantify the 146S antigen in inactivated foot-and-mouth disease vaccine / 生物工程学报

The aim of this study is to quantify the 146S antigen in foot-and-mouth disease virus (FMDV) inactivated vaccine by size-exclusion chromatography (SEC). The analysis was performed on a TSKgel G4000SWXL column (7.8 mm×30 cm), with a pH 7.2 buffer salt system as the mobile phase. The flow rate was 0.6 mL/min, the injection volume was 100 μL and the detection wavelength was 259 nm. The calibration curve was established by using purified inactivated FMDV (serotype O) 146S antigen; 3 batches of vaccine formulated by inactivated antigen solution were tested to verify the accuracy, reproducibility, specificity and tolerability of the method. At last 16 batches of vaccine were determined by the SEC method. Results showed a good linearity between peak area and concentration of 146S antigen in the range between 0.56 and 67.42 μg/mL (R2=0.996, n=10), and the average recovery rate of 146S antigen in the 3 batches of vaccine formulated in lab were 93.6% (RSD=2.7%, n=3), 102.3% (RSD=2.6%, n=3), and 95.5% (RSD=5.1%, n=3). The method was proved accurate and reliable with good reproducibility (RSD=0.5%, n=6), and applied to determine 16 batches of the commercial FMDV vaccine. According to the above results, the SEC method is high effective for 146S antigen quantify in the inactivated FMDV vaccine and would provide strong support for the vaccine quality control.

Cuidado de enfermagem na percepção de usuárias de um serviço público de saúde em Salvador (BA) / Nursing care as perceived by patrons of a public health service in Salvador (BA) / El cuidado de enfermería según la percepción de usuarias de un servicio de salud pública de Salvador (BA)

O presente estudo teve como objetivo descrever o cuidado de enfermagem às mulheres no pré-natal, com base napercepção de gestantes acompanhadas em um Programa de Saúde da Família do município de Salvador (BA). Os sujeitosforam onze mulheres assistidas pelo menos duas vezes pela enfermeira no atendimento pré-natal. Como técnica de coletade dados, utilizou-se a entrevista semiestruturada. A análise dos dados foi realizada pela técnica de análise temática dosrelatos orais das gestantes. Os resultados apontaram a valorização da consulta de enfermagem pelas usuáriasconcebendo-a como atividade sistematizada e resolutiva; a utilização de tecnologias de relação qualifica o cuidado aopromover um tipo de relação interpessoal cordial e interessada; as discussões em grupo são consideradas comoestratégias importantes tanto do ponto de vista educativo/informativo, quanto terapêutico para o cuidado pré-natal.

The objective of the study was to describe nursing care to prenatal women, based on the perception of the pregnantwomen in a Family Health Program in the city of Salvador (BA). The subjects of the study were 11 women treated bythe prenatal nurse at least twice per week. Semi-structured interviews were used for data gathering. The data wasanalyzed using the thematic analysis technique for the oral statements of the participants. The results illustrated: theimportance of the nursing assistance given by the participants, classifying it as a systematized and resolutive activity;the utilization of related technologies, which qualify the care provided by promoting caring and cordial interpersonalrelations; and group discussions are considered important strategies from the educational/informative perspective, aswell as therapeutic for prenatal care.

El presente estudio tuvo como objetivo describir el cuidado de enfermería a las mujeres en el prenatal, con base en lapercepción de gestantes acompañadas en un Programa de Salud de la Familia del municipio de Salvador (BA). Los sujetos fueron once mujeres asistidas por la enfermera por lo menos dos veces en el atendimiento prenatal. Como técnicade colecta de datos, se utilizó la entrevista semiestructurada. El análisis de los datos fue realizada por la técnica deanálisis temático de los relatos orales de las gestantes. Los resultados indicaron la valorización de la consulta deenfermería por las usuarias concebiéndola como actividad sistematizada y resoluta; la utilización de tecnologias derelación cualifica el cuidado al promover un tipo de relación interpersonal cordial e interesado; las discusiones engrupo son consideradas como estrategias importantes tanto del ponto de vista educativoinformativo, cuantoterapéutico para el cuidado prenatal.