Isolation and identification of chemical constituents from Disporum cantoniense (II) / 中草药

Objective To investigate the chemical constituents from Disporum cantoniense. Methods Compounds were isolated and purified by macroporus resin, ods, Sephadex LH-20, MCI-gel CHP20 resin column chromatography and preparative HPLC. The structures were identified by spectral analysis. Results Twenty compounds were isolated and identified as 2-hydroxybenzyl alcohol (1), p-hydroxybenzaldehyde (2), 4-hydroxyacetophenone (3), 3,5-dimethoxy-4-hydroxy-benzaldehyde (4), 4-(4-hydroxyphenyl)-2- butanone (5), neoliquiritin (6), 2,3,5,4’-tetrahydroxy stilbene-2-Ο-β-D-glucoside (7), (E)-1-(4’-hydroxyphenyl)-but-1-en-3-one (8), isoquercitrin (9), 3-hydroxy-1-(4-hydroxy-3,5-dimethoxy-phenyl)-1-propanone (10), icariol A2 (11), ecdysterone (12), glansreginic acid (13), hesperidin (14), ononin (15), quercetin (16), isorhamnetin-3-O-β-D-glucoside (17), (E)-4-(4-hydroxy-3-methoxyphenyl) but-3-en-2-one (18), (-)-secoisolariciresinol (19), and luteolin (20). Conclusion Compounds 1, 3-8, 10-15, and 17-19 are isolated from the genus of Disporum for the first time.

Chemical constituents in stems of Ilex cornuta / 中草药

Objective: To investigate the chemical constituents in the stems of Ilex cornuta and the ability of scavenging free radicals of compounds 1-9. Methods: The compounds were isolated and purified by silica gel, medium pressure column chromatography, and semi-preparative liquid chromatography, and their structures were elucidated by chemical properties and spectroscopic analyses. The antifreeradical efficiency of compounds 1-9 was evaluated by DPPH radical scavenging assay. Results: Fifteen compounds were isolated and their structures were identified as isochlorogenic acid B (1), 3,4,5-tricaffeoylquinic acid (2), 4,5-di-O-caffeoyl quinic acid methyl ester (3), 3,4-di-O-caffeoyl quinicacid methyl ester (4), 3,5-di-O-caffeoyl quinicacid methyl ester (5), 3,4,5-tri-O-caffeoyl quinic acid methyl ester (6), 3,5-dimethoxy-4-hydroxybenzaldehyde (7), ethyl gallate (8), dihydrosyringenin (9), 2,6-dimethoxy-1,4-benzoquinone (10), arctigenin (11), 1-O-(vanillic acid)-6-O-(3″, 5″-dimethoxy-galloyl)-β-D-glycoside (12), (+)-1-hydroxypinoresinol-1-O-β-D-glucopyranoside (13), (+)-(7S,8S)-syringylglycerol 8-O-β-D-glucopyranoside (14), and schaftoside (15). Compounds 1-7 had good antifreeradical efficiency. Conclusion: Compounds 6,8-10,14, and 15 are obtained from the plants of Ilex L. the first time, and compounds 2,7,11, and 12 are obtained from this plant for the first time. Compounds 1-6 have good antifreeradical efficiency.

Direct and derivative spectrophotometric determination of gold (iii) using 3,5-dimethoxy-4-hydroxybenzaldehyde benzoylhydrazone(dmbbh) in presence of micellar medium.

A simple and sensitive spectrophotometric method has been developed for the determination of Gold (III) using 3,5-Dimethoxy-4-hydroxy benzaldehyde benzoylhydrazone (DMBBH) in a neutral surfactant of Triton X-100(5%) (micellar medium). Gold (III) forms an wine red coloured water soluble complex with the reagent in acidic medium pH 5.0. The molar absorptivity and Sandell’s sensitivity of coloured species are 2.32 x 104 L.mol-1 cm-1 and 0.00862 mg/cm2 respectively. Beer’s law is obeyed in the range 0.4925-5.91 mg/ml of Au(III) at lmax at 380 nm. The developed derivative spectrophotometric method was employed for the determination of gold (III). This method has been satisfactorily applied for the determination of gold (III) in water samples and pharmaceutical samples.