RESUMEN
Objective: To establish a UPLC fingerprint of Compound Qima Capsule (CQC), to determine the eight contents in CQC (gastrodin, 5-hydroxymethylfurfura(5-HF), calycosin-7-O-β-D-glycoside(CG), rhoifolin, naringin, formononetin-7-O-β-D-glucoside (FG), calycosin, and formononetin), and to provide the basis for the evaluation of CQC. Methods: The Agilent Eclipse C18 (100 mm × 4.6 mm, 3.5 μm) column was used with a mobile phase of methyl acetonitrile-0.05% phosphoric acid gradient elution, the flow rate was 0.3 mL/min, the column temperature was 25℃, and the detection wavelength was 265 nm. Results: The fingerprint chromatography with good resolution and reproducibility included 22 mutual peaks, and the similarity was more than 0.98. Gastrodin, 5-HF, CG, rhoifolin, naringin, FG, calycosin, and formononetin were baseline seperated with good linearity relationships between concentration and peak areas over the linear ranges, within 0.976-29.880, 10.596-52.980, 2.697-13.485, 2.262-11.309, 40.768-203.840, 5.825-29.126, 0.372-1.858, and 1.888-9.440 μg/mL (r > 0.999 9), whose average recoveries were 1.04%, 1.30%, 1.81%, 1.41%, 1.29%, 1.01%, 1.48%, and 1.29%. The contents of 10 batches of gastrodin, 5-HF, CG, rhoifolin, naringin, FG, calycosin, and formononetin were 2.883-3.491, 1.710-3.791, 0.107-0.286, 0.157-0.346, 8.853-10.726, 0.282-0.692, 0.097-0.135, and 0.041-0.063 mg/g, respectively. Conclusion: The method is rapid, simple, and accurate, and can be used for the quality control of CQC.