RESUMEN
AIM: To establish a HPLC method of determining prim-o-glucasylcimifugin and 5-o-methyl-visammiosode in Yinhua Ganmao Granules (Flos Lonicerae Japonicae, Radix Saposhnikovial, Fructus Forsythiae, etc.). METHODS: A Kromasil C_(18) column was used. The mobile phase was MeOH-Acetonitrile-H_2O(30∶15∶80). The detection wavelength was 254 nm. RESULTS: The linear range of prim-o-glucasylcimifugin was within 0.157 5-3.780 5 ?g(r=0.999 9), The linear range of 5-o-methylvisammiosode was within 0.164 4-3.945 6 ?g(r=0.999 8). The average recoveries(n=6) were 99.40% and 96.79%, respectively. RSD were 0.6% and 0.8% respectively. CONCLUSION: The method is simple, reliable, accurate and can be applied to the quality control of the preparation.
RESUMEN
AIM: To establish a method of determing prim-o-glucasylcimifugin,5-o-methylvisammiosode,gardenoside quantitatively in Huoxiang Gingwei Granules(Herba Pogostemonis,Fructus Gardeniae,Radix Saposhnikoviae,etc.). METHODS: Three components were determined by HPLC in the same chromatographic condition(Column: Shimadzu VP-ODS 250 mm?4.60 mm,5 ?m;mobile phase: acetonitrile-0.1% phosphoric acid gradient elution;flow rate: 1.0 mL/min: column temperature: at 30 ℃). RESULTS: Prim-o-glucasylcimifugin showed a good linear relationship at the range of 26.0-390 ng,r=(0.999 9),the average recovery was 101.7% and RSD was 1.1%.5-o-methylvisammiosode showed a good linear relationship at the range of 21.5-322.2 ng,r=(0.999 9),the average recovery was 101.9% and RSD was 1.1%.Gardenoside showed a good linear relationship at the range of 47.1-706.5 ng,r=(0.999 9),the average recovery was 101.6% and RSD was 1.1%.(CONCLUSION):() The established method can provide the content determination of 3 ingriedents at the same test condition and be used for the quality control of Huoxiang Qingwei Granules.