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Objective: To study the chemical constituents of aerial part of Gendarussa vulgaris. Methods: The compounds were separated and purified by silica gel column chromatography, ODS and Sephadex LH-20 chromatography and semi-preparative HPLC. Base on HR-ESI-MS, NMR, and other spectral data, their structures were identified. Results: A total of 17 compounds were isolated from the EtOAc fraction of 95% ethanol extract and identified as 24-norchol-5-en-3β-ol (1), dihydrobetulic acid (2), betulinic acid (3), 3-hydroxy-30-nor-20-oxo-28-lupanoic acid (4), 6-hydroxy-7,8-dimethoxycoumarin (5), 6,7-dimethoxycoumarin (6), 5,6,7- trimethoxycoumarin (7), 6,7,8-trimethoxycoumarin (8), syringaresinol-4-O-β-D-glucopyranoside (9), 4-O-caffeoylquinic acid methyl ester (10), N-trans-feruloyl tyramine (11), N-(2-hydroxy-3-phenylpropyl) acetamide (12), 3-O-caffeoylquinic acid methyl ester (13), 3,5-O-dicaffeoylquinic acid methyl ester (14), p-E-coumarin quinic acid methyl ester (15), 3,4,5-O-tricaffeoyl quinic acid methyl ester (16) and 1'S*,4'R*-8-(4'-hydroxy-2',6',6'-trimethylcyclohex-2-enyl)-6-methyloct-3E,5E,7E-trien-2-one (17). Conclusion: Compounds 1 and 2 are new natural products. All Compounds are isolated from this plant for the first time except compound 6. Besides, all compounds are screened for anti-inflammatory activity and compounds 2, 3, 11, 13, and 17 have NO release inhibiting activities on LPS-induced RAW 264.7 macrophage cells with IC50 values of (30.91 ± 0.50), (44.66 ± 0.56), (17.67 ± 0.57), (28.45 ± 0.67) and (20.79 ± 0.24) μmol/L, respectively.
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OBJECTIVE：To establish a method for sim ultaneous determination of 7-hydroxycoumarin，6，7-dimethoxy- coumarin，5，7-dimethoxycoumarin，imperatorin and bergaptene in Shugan hewei pills. METHODS ：Samples were extracted by Soxhlet extraction and separated by using D 101 macroporous adsorption resin ；HPLC method was used to determine the contents of above 5 components. The determination was performed on Venusil MP C 18 column with mobile phase consisted of methanol- 0.1％ phosphoric acid water solution （gradient elution ）at the flow rate of 1.0 mL/min. The detection wavelength was 270 nm and the column temperature was 30 ℃ . The sample size was 10 μ L. RESULTS：The linear ranges were 1.875-187.5 μ g/mL for 7-hydroxycoumarin（r＝0.999 9），1.075-107.5 μg/mL for 6，7-dimethoxycoumarin（r＝0.999 9），1.261-126.1 μg/mL for 5， 7-dimethoxycoumarin（r＝0.999 9），0.875-87.5 μg/mL for imperatorin（r＝0.999 9），2.033-203.3 μg/mL for bergaptene（r＝0.999 8）， respectively. RSDs of precision ，stability（24 h），reproducibility and durability tests were lower than 2.0％. Average recoveries were 95.18％ -98.44％ （RSD＝1.27％ ，n＝6）， 95.35％ -98.14％ （RSD＝1.16％ ，n＝6），95.21％ -97.32％ （RSD＝0.83％ ，n＝6）， 95.43％-97.14％（RSD＝0.76％，n＝6），95.12％-98.75％（RSD＝1.35％，n＝6），respectively. The average contents of the above five components in 3 batches of shugan hewei pills were 0.424，0.239，0.305，0.180，0.459 mg/g（n＝2）.CONCLUSIONS：A method for simultaneous determination of five components in Shugan hewei pills was established.
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Objective: To study the chemical constituents in the leaves of Magnolia officinalis var. biloba. Methods: Compounds were isolated by various chromatographic techniques and identified by spectral analyses. Results: Twelve compounds were isolated in the 75% ethanol extract from the leaves of M. officinalis var. biloba and identified as lirioresinol-A (1), 6, 7-dimethoxycoumarin (2), indole-3-aldehyde (3), S-(+)-dehydrovomifoliol (4), loliolide (5), magnolol (6), trans-p-coumaryl aldehyde (7), honokiol (8), 4-allylphenol (9), nonacosanoic acid (10), grasshopper ketone (11), and syringin (12). Conclusion: Compounds 1-5, 7, 9-11 are first isolated from the leaves of M. officinalis var. biloba and plants of this genus.
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Objective: To investigate the chemical constituents from the dried roots and rhizomes of Physochlaina infundibularis. Methods: Repeated chromatography with silica gel, macroporous resin, MCI, and Sephadex LH-20 columns was used to isolate the chemical constituents and their structures were elucidated based on physicochemical properties and spectral data. Results: Seventeen compounds were isolated and identified as hyoscyamine (1), scopolamine (2), anisodamine (3), scopoletin (4), scopolin (5), umbelliferone (6), 6, 7-dimethoxycoumarin (7), 3-methoxyquercetin (8), isoquercitin (9), kaempferol-7-O-β-D-glucoside (10), syringarenol (11), protocatechuic acid (12), methyl 4-hydroxybenzoate (13), 2-hydroxybenzoic acid (14), tropic acid (15), 4- hydroxybenzoic acid (16), and palmitic acid (17). Conclusion: Compounds 7-17 are firstly obtained from the plants in Physochlaina G. Don, and compound 5 is firstly obtained from P. infundibularis.
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Objective To study the rational daily administration times of Yinchenhao Decoction (YCHD) when it was used to treat experimental jaundice in rats based on pharmacodynamics/pharmacokinetics model.MethodsRats were modeled by 4％ 1-naphthylisothiocyanate (75 mg/kg) for 48 h,then YCHD was drenched with doses of 0.324 g/kg (extract,calculated with the clinical dosage) once,0.162 g/kg twice,and 0.108 g/kg thrice a day,respectively.The total bile and the flow rate of bile were observed after the first administration; Blood samples collected from the orbital sinus at different intervals were used to investigate the levels of liver enzymes (ALT and AST) and bilimbins (TBIL and DBIL),and determine the concentration of 6,7-dimethoxycoumarin (DME) in the plasma using UPLC at the same time,then we obtained the time-effect and time-dose curves.The rational daily administration times of YCHD when treating experimental jaundice were determined based on the comprehensive analysis of time-effect and time-concentration relationships.ResultsWithin 10 h the total bile of rats which were administered once daily (G1) was I.65 and 1.33 times higher than that of twice and thrice (G2 and G3) a day,respectively,and the four biochemical indexes (TBIL,ALT,DBIL,and AST) of Gl decreased faster than those of G2 and G3 (P ＜ 0.05).On the other hand,the blood drug level of DME when administrated once daily could maintain at a higher level for a longer time,and its Cmax and AUC0→t were higher than those of G2 and G3,which might be the main reason why its effect was the most significant.ConclusionIt is more appropriate to administrate once daily when YCHD is used to treat jaundice.
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Objective: To study the plasma concentrations and pharmacokinetic parameters of 6,7 dimethoxycoumarin after oral administration of Herba Artemisiae Scopariae decoction and Yinchenhao decoction. Methods: A HPLC UV method was developed to determine 6,7 dimethoxycoumarin in rat plasma. The drug was extracted with acetonitrile from plasma and separated on a Kromasil ODS (250mm?4.6mm,5?m)column with methanol 1% solution of acetic acid THF (30∶63∶7) as mobile phase, UV detecter was set at 340nm. The data obtained were analyzed with Topfit program. Results: The liner calibration curves were obtained in the concentration range of 0.026～2.5 ?g?mL -1 . With non compartmental analysis, the main pharmacokinetic parameters after oral administration of Artemisiae Scopariae decoction and Yinchenhao decoction were as follows: T 1/2 was 1.30 and 1.75h, AUC 0→t was 1215.00 and 2527.85ng?h?mL -1 , AUC 0→∝ was 1325.90 and 2612.58ng?h?mL -1 respectively. Conclusion: This method can be used to study the pharmacokinetics of 6,7 dimethoxycoumarin derived from different traditional Chinese medicines containing this component.