Suspension culture of Vero cells for the production of adenovirus type 5

Clinical Efficacy of Endoscopic Injection of Recombinant Human Adenovirus Type 5 for Advanced Esophageal Cancer / 胃肠病学

Background: Recombinant human adenovirus type 5 is an oncolytic adenovirus, which can selectively replicate in tumor cells and lyse the tumor cells resulting in dissolution and necrosis of tumor tissues. Aims: To evaluate the efficacy and safety of endoscopic injection of recombinant human adenovirus type 5 in the treatment of advanced esophageal cancer. Methods: A total of 87 patients with advanced esophageal cancer from January 2012 to October 2017 at Qingdao Municipal Hospital were enrolled. According to the treatment performed, patients were divided into recombinant human adenovirus type 5 group (group A), radiation and chemotherapy group (group B) and recombinant human adenovirus type 5 combined with radiation and chemotherapy group (group C). The clinical efficacy, survival rate and adverse reaction rate were compared among the three groups. Results: No significant differences in clinicopathological features were found among the three group (P>0.05). The effective rate, 1-year survival rate, 2-year survival rate, median overall survival, median progression-free survival in groups C were significantly higher than those in group A and group B (P0.05). Adverse reaction rate in group A was significantly decreased than that in group B and group C (P0.05). Conclusions: The therapeutic effect of endoscopic injection of recombinant human adenovirus type 5 combined with radiation and chemotherapy for treatment of advanced esophageal cancer is remarkable, and is an important novel way for the treatment of advanced esophageal cancer, which does not increase obviously the incidence of adverse reactions.

Application of recombinant adenoviruses expressing glycoprotein or nucleoprotein of rabies virus to Korean raccoon dogs

PURPOSE: A new rabies vaccine for animals, including raccoon dogs, in Korea is needed to eradicate rabies infection. In this study, we constructed two recombinant adenoviruses expressing the glycoprotein or nucleoprotein of the rabies virus (RABV). We then investigated the safety and immunogenicity of these strains in raccoon dogs, depending on inoculation route. MATERIALS AND METHODS: Recombinant adenoviruses expressing the glycoprotein (Ad-0910G) or nucleoprotein (Ad-0910N) of rabies were constructed in 293A cells using an adenoviral system. One-year-old raccoon dogs underwent intramuscular (IM) inoculation or oral administration of the recombinant Ad-0910G and Ad-0910N. Clinical symptoms were observed and virus-neutralizing antibodies (VNA) against RABV were measured at 0, 2, 4, and 6 weeks after the immunization. Raccoons were considered positive if VNA titers were > or = 0.1 IU/mL. RESULTS: Raccoon dogs inoculated with the combined Ad-0910G and Ad-0910N virus via the IM route did not exhibit any clinical sign of rabies during the observation period. All raccoon dogs (n = 7) immunized IM had high VNA titers, ranging from 0.17 to 41.6 IU/mL at 2 weeks after inoculation, but 70% (7/10) of raccoon dogs administered viruses via the oral route responded by 6 weeks after administration against RABV. CONCLUSION: Raccoon dogs inoculated with Ad-0910G and Ad-0910N viruses showed no adverse effects. Immunization with the combined Ad-0910G and Ad-0910N strains may play an important role in inducing VNA against RABV in raccoon dogs.

Human Adenovirus Type 5 as a Delivery Vector is Not Neutralized in Field Serum Samples of Cattle, Pig, and Goat of Republic of Korea

Human adenovirus type 5 (hAd5) vectors have been demonstrated to be useful vehicles for gene expressions in animals. However, it has not been reported whether hAd5 transduction might be hampered in the sera of livestock animals in Republic of Korea. We collected 205 samples of livestock animals, such as pig (n=84), cattle (n=84), and goat (n=37) in Korea. The neutralizing antibody (NAb) titers to hAd5 virus were less than 15 in most of samples. Only 8% of goat samples had a NAb titer of 15 or 30. Thus, we showed that hAd5 virus was not neutralized in sera from cattle, pig, and goat, and suggest that the hAd5 vector could be used for the effective delivery of vaccines or proteins in livestock animals in the field.