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The study was intended to investigate anti-diabetic efficacy of Aerva lanata by determining its α-amylase inhibition activity and in vitro uptake of glucose in adipose tissue and psoas muscle isolated from male Sprague Dawley (SD) rats. Aerva lanata is reported to have many traditional and Ayurvedic uses. Male SD rats (n=3) of 150 g were sacrificed and 250 mg of respective tissues were isolated for the study. Aerva lanata ethanolic extract (ALE) (5-20 mg/mL) showed 13.30 to 54.08% α-amylase inhibition activity. Glucose uptake studies in in vitro conditions were carried out in both adipose tissue and psoas muscle in different sets - tissue alone, tissue along with (Aerva lanata extract: 50µg, 100µg, 150µg, insulin: 25 mU/L, insulin: 50 mU/L and Aerva lanata extract: 50µg + insulin: 25 mU/L, Aerva lanata extract: 100µg + insulin: 25 mU/L, Aerva lanata extract: 150µg + insulin: 25 mU/L, Aerva lanata extract: 50µg + insulin: 50 mU/L, Aerva lanata extract: 100µg + insulin: 50 mU/L, Aerva lanata extract: 150µg + insulin: 50 mU/L). The rate of glucose uptake by insulin action in these tissues was stabilized by ethanolic extract of Aerva lanata and this shows synergetic activity of insulin and Aerva lanata.
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All the plants are having medicinal values and thus are used traditionally in many diseasesfrom ancient times. One of such useful plant is Aerva lanata commonly known as “Bhui” which is awoody, prostate or succulent, perennial herb from the Amaranthaceae family, found in open forest onmountains, slopes, disturbed ground and deserted areas(1). The plant has been screened for diuretic,antidiabetic, anti-inflammatory and hepatoprotective activity (2) (3). According to the literature referredthe pharmacognostical studies of this plant have not been reported yet, therefore the presentinvestigation is planned to study the pharmacognostical and phytochemical aspects of Aerva lanata. Inthe present research article the pharmacognostical study i.e. morphological, microscopical, chemical &chromatographic analysis of plant Aerva lanata was carried out. This study provides the standardizationparameters important for the characterization & identification of the plant. The information will beuseful for the traditional medicine practitioners & establishing literature regarding the plant.Microscopic studies shows upper epidermis is straight walled, single layered containing trichomesbelow the epidermis collenchyma cellular layers are present which can be characterized by thickcellulosic deposition. Cells contain calcium oxalate crystals (in small amount) and starch. Vascularbundles present in spongy tissues. Physiochemical analysis shows Total ash, Acid insoluble ash, Watersoluble ash, Sulphated ash values as 10.01%, 2.01%, 4.92% and 4.82% respectively. Other parameterslike Alcohol soluble extractive value, Water soluble extractive value, Loss on drying and swelling indexare found to be 20%, 24%, 8% and 6.42%. Fluorescence study and preliminary phytochemical tests arealso performed. Thin layer chromatographic studies showing presence of carbohydrates, steroids,flavonoids and tannins at Rf values of 0.88, 0.86, 0.92 and 0.86 respectively.
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Aim: To determine the phytochemical constituents present in the different parts of Aerva lanata using Gas Chromatography – Mass Spectrometry (GC-MS). Study Design: GC-MS analysis of bioactive compounds in different parts of A. lanata. Place and Duration of Study: Post Graduate and Research Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam and Department of Food Safety and Quality Testing, Indian Institute of Crop Processing Technology, Thanjavur, Tamilnadu, India, between May 2011 to June 2012. Methodology: 15 g of powdered plant material of leaf, flower and root were soaked with 60 mL of 95% ethanol for 24 hrs. After 24 hrs, the extract was filtered and the filtrate was concentrated to 1 mL by bubbling nitrogen gas into the solution. 2 μL of ethanolic extracts of leaf, flower and root of A. lanata was used for GC-MS analysis. Results: The GC-MS analyses showed that the presence of four different phytocompounds in ethanolic extract of leaf of A. lanata. The highest peak area of 74.73% for isophytol was identified in leaf of A. lanata. The ethanolic flower extract of A. lanata showed that the presence of twelve different phytocompounds. Flower extract contains the highest amount of phytocompound was 6, 9,12 –octadecatrienoic acid, phenyl methyl ester (z,z,z)- with the peak area of 25%. The root extract of A. lanata showed that the presence of eight different bioactive compounds. The root of A. lanata showed more quantity of lanost-9 (11)-en-12-one with the highest peak area of 45.11%. Conculsion: The present study confirmed that the presence of active compounds in different parts of A. lanata. In future, the isolation of above mentioned bioactive compounds from the different part of A. lanata would be useful to find out the novel drugs.
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The sorption abilities of leaves powders of Bhringraj , Aerva lanata, Trianthema portulacastrum L for extracting Chromium (VI) from polluted waters have been studied with respect to various physicochemical parameters such as pH, sorption dosage and equilibrium time. The conditions for maximum removal of Chromium (VI) have been optimized. Ten fold excess of common cation ions present in natural waters, viz., Ca2+, Mg2+ , Cu2+, Zn2+, Ni2+ and Fe2+ have synergistic effect in increasing the % removal of Chromate. SO4 2—and Phosphates are found to be interfering with the extractability of Chromates but NO3 - , Chloride, Fluoride and Carbonate have marginal interference. However, the extraction has never come down below 72.0%. Maximum extractions to an extent of 96.0%, 92.0%, and 84.0% from synthetic waters are observed with the leaves powders of Bhringraj , Aerva lanata, Trianthema portulacastrum L respectively at pH:2 and at optimum equilibration time and sorbent concentrations. The methodologies developed are applied to diverse waste water samples collected from industrial effluents and polluted lakes. The procedures are found to be remarkably successful in removing the Chromiume(VI) from waste waters.
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Objective: To identify the flavonoids HPTLC profile (bio-marker), at species level, for the identification and confirmation of crude drugs, HPTLC separation was initiated on different parts of Aerva lanata (A. lanata) L. from South India. Methods: Preliminary phytochemical screening was done by following the method of Harborne. HPTLC studies were carried out following Harborne and)Wagner et al method. The ethyl acetate-butanone-formic acid-water (5:3:1:1) was employed as mobile phase for flavonoids. Results: The methanolic extract of stem, leaves, root, flower and seeds of A. lanata showed the presence of 19 different types of flavonoids with 19 different Rf values with range 0.05 to 0.98. In general more degree of flavonoids diversity has been observed in vegetative parts when compared to the reproductive part. Maximum number of flavonoids has been observed in leaves followed by root and leaves. Conclusions: The results of the present study supplement the folkloric usage of the studied plants which possess several known and unknown bioactive compounds with bio-activity. By isolating and identifying these bioactive compounds new drugs can be formulated to treat various diseases. It can be concluded that constituents of A. lanata have effective components which can be utilized as useful herb for alleviation of various illness and disorder.
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<p><b>OBJECTIVE</b>To determine the chemical profile and steroids composition of the medicinally important plant Aerva lanata (A. lanata) L.</p><p><b>METHODS</b>Preliminary phytochemical screening was done by the method as Harborne described. HPTLC studies were carried out as Harborne and Wagner et al described. The Ethyl acetate-ethanol-water (8: 2: 1.2) was employed as mobile phase for glycosides.</p><p><b>RESULTS</b>The desired aim was achieved using Chloroform-acetone (8: 2) as the mobile phase. The methanolic extract of stem, leaves, root, flower and seeds of A. lanata showed the presence of 30 different types of steroids with 30 different Rf values from 0.04 to 0.97. Maximum number (11) of steroids has been observed in leaves followed by root (10).</p><p><b>CONCLUSIONS</b>HPTLC profile of steroids has been chosen here to reveal the diversity existing in A. lanata. Such finger printing is useful in differentiating the species from the adulterant and act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.</p>