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ObjectiveTo explore the material basis and mechanism of Nardostachyos Radix et Rhizoma （NRER）-Agrimoniae Herba （AH）， the herbal pair effective in regulating the liver， invigorating Qi， and calming palpitations， in the treatment of premature ventricular contractions （PVCs） by network pharmacology and molecular docking. MethodThe chemical components and targets of NRER and AH were collected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP） combined with relevant literature. GeneCards，Online Mendelian Inheritance in Man（OMIM），and DrugBank were used to predict the potential targets against PVCs. STRING platform was used for protein-protein interaction （PPI） analysis. Metascape platform was used for Gene Ontology（GO） and Kyoto Encyclopedia of Genes and Genomes（KEGG） enrichment analysis. Cytoscape 3.8.0 was used to construct the NRER-AH component-potential target-signaling pathway network. The main target proteins underwent molecular docking to the active components of NRER-AH by AutoDock 4.2.6. ResultThe targets of nine active components in NRER-AH （such as quercetin，kaempferol，and acacetin） against PVCs mainly involved tumor necrosis factor （TNF），mitogen-activated protein kinase 1（MAPK1），and protein kinase B1（Akt1）. The potential targets were mainly enriched in 26 signaling pathways，such as pathways in cancer and the advanced glycosylation end product （AGE）-receptor of advanced glycosylation end product（RAGE） signaling pathway. The results of molecular docking showed that the majority of the active components （92.59%） of NRER-AH had good binding activities with the main target proteins TNF，MAPK1，and Akt1. ConclusionThe active components of NRER-AH can regulate cardiac ion channels，resist inflammation， and combat oxidative stress to treat PVCs through multi-target and multi-pathway interventions. They can also improve symptoms related to depression and anxiety by inhibiting monoamine oxidase activity and protecting nerves from damage. This study is expected to provide research ideas and the theoretical basis for further exploring the material basis and mechanism of NRER-AH in the treatment of PVCs.
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Objective: To establish an HPLC fingerprint of methanol extract of Zhixue Tiaojing Granule (ZTG), and determine the content of paeoniflorin and calycosin-7-glucosid. Methods: HPLC analysis was performed on an Thermo UltiMateTM U3000 LC system and carried out at 30 ℃ on a column of Thermo AcclaimTM 120 C18 (250 mm × 4.6 mm, 5 μm). Gradient elution system was composed of water solution (phase A) and acetonitrile (phase B). Detection was performed at the wavelength of 254 nm, the mobile flow rate was 1 mL/min, the column temperature was 30 ℃, and sample volumn was 10 μL. Results: The HPLC fingerprint for 10 batches of ZTG were obtained and the similarities for 10 batches of samples were all over 0.95. Eleven characteristic peaks were found and peaks were from Astragali Radix (peak 1, 5, 7, 10), Codonopsis Radix (peak 1), Dipsaci Radix (peak 2, 3), Paeoniae Radix Alba (peak 4), Angelicae Sinensis Radix (peak 6), Rubiae Radix et Rhizoma (peak 6, 8, 11), and Agrimoniae Herba (peak 9). Besides, three peaks were assigned include paeoniflorin, calycosin-7-glucosid, and formononetin. The content of paeoniflorin and calycosin-7-glucosid was 1 062 μg/g and 84.08 μg/g. Conclusion: The method can be used for the quality control of ZTG with good precision, accuracy, and reproducibility.
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Potentillae Discoloris cum Radice Herba and Agrimoniae Herba are closely-related medicinal plants in Rosaceae with specific hypoglycemic activities and applied as Chinese materia medica in clinical practice for the treatment of diabetes. Based upon pharmaphylogeny theories, the biosynthetic pathways of the principal active components of the above two Chinese herbs were summarized, and the commonness and differences of the active components were compared further and the correlation between material basis and curative effects was summarized as well. The results indicated that the common biosynthetic pathways of the two Chinese herbs accounted for 60%; The common components of tiliroside and quercetin, etc. as well as the differentiated components of potengriffioside A and rosolic acid, etc. are also the active components; The differences of the characteristic components and their contents may lead to the differences of functions and modes of action between the two Chinese herbs.