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OBJECTIVE:To investigate the protective effect of meloxicam on aluminum overload-inducing damage of hippocampal neuron.METHODS:Primary hippocampal neuron attained from new born SD rats was cultured for 7 days.There were 5 groups e.g blank control group (200 ?mol?L-1 NaCl),aluminum model group (200 ?mol?L-1 AlCl3) and meloxicam low-dose (10-8 mol?L-1),medium-dose (10-7 mol?L-1) and high-dose (10-6 mol?L-1) groups.HE staining was used to observe the change of neuronal morphology.The optical densities of cells were measured.The activities of SOD,leakage of LDH and content of MDA were also detected.RESULTS:As compared with control group,the optical density of cells and activity of SOD were decreased and leakage of LDH and content of MDA were increased (P
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AIM:The objective of the study was to explore whether intestinal endotoxemia participate in the development of Alzheimer disease.METHODS:Adult Wistar rats were subjected to 90 days intraperitoneal injection with D-galactose and aluminum trichloride(AlCl3) to establish the model of Alzheimer disease.After the administration,the study and memory ability in the rats were observed by Morris water maze.The level of lipopolysaccharide(LPS) in the sera of Alzheimer disease's rats was determined by tachypleus amebocyte lysate method.The level of tumor necrosis factor-?(TNF-?) and interleukin-1(IL-1) in the sera were determined by radioimmunoassay.The expressions of amyloid ?-protein precursor(APP),presenilin 1(PS1) and ?-site APP-cleaving enzyme(BACE) in hippocampus were detected by RT-PCR.RESULTS:Compared with the normal control,the level of LPS in the sera and the expressions of APP,PSI,BACE mRNA in the hippocampus were markedly increased(P