RESUMEN
Objective: To explore the applicability of nanofiltration separation of salvianolic acid B in organic phase. Methods The rejection rate, mass transfer coefficient, membrane flux, membrane durability and membrane integrity were selected as indexes to evaluate the applicability of nanofiltration for the separation of salvianolic acid B raw material in organic phase from the eluate of macroporous resin. Results The experiment showed that there was a negative relationship between rejection rate and membrane molecular weight cut-off. Meanwhile, the mass transfer coefficient of salvianolic acid B was decreased and the membrane flux was obviously decayed with the increase of ethanol concentration. Through the membrane durability and integrity evaluation, the rejection performance of NFG nanofiltration membrane was stable with the increasing of separation time. Also, the membrane flux decreased first and then increased and maintained stable. At the same time, the charge characteristic of the membrane separation layer was stable, but the effective separation aperture was increased by the combined effects of membrane fouling, additives shedding and so on. Conclusion Based on the comprehensive analysis, NFG nanofiltration membrane could meet the requirements of the separation of low concentration ethanol solution, and had good applicability. The applicability of nanofiltration separation in organic phase was analyzed comprehensively with salvianolic acid B as example, providing theoretical and technical support for nanofiltration separation of phenolic acids in organic phase.
RESUMEN
Objective To prepare pH sensitive gel for eye cornea of salvianolic acid B and accomplish in vitro release study. Me thods We used corneal permeability, eye irritation in rabbits, hydration of the cornea, viscosity, gel capacity, transparency, liquidity as index, to select pH sensitive gel matrix composition. Re s ults The gel composition was: 0.5％ of salvianolic acid B, 0.14％ Carbomer, 0.3％ HPMC, 0.05％ Azone, 0.1％ ethylparaben and 0.2％ Vc. The corneal hydration level was 77.32±1.88.The gel released 27.21％ drug in 15 min, and released 97.44％ drug in 6 h. By research of corneal permeability, the cumulative release of gel was bigger than the one of saline (P＜0.05). Conclus ions The selection of the gel matrix prescription is reasonable in design, simple in process, not harsh to eyes, and can prolong the retention time of drug in corneas and improve the corneal penetration.