RESUMEN
Objective To observe the effects of three effective constituents of Min Angelicae Sinensis Radix on hyperhomocysteinemia (HHcy) induced rabbit atherosclerotic lesions (AS).Methods Forty eight Japanese big ear rabbits were randomly divided into normal group, model group, Angelicae Sinensis Radix volatile oil group, Angelicae Sinensis Radix polysaccharide group, ferulic acid group and folic acid group, with 8 rabbits in each group. The HHcy rabbit model induced AS was duplicated by injected DL-methionine. After 8 weeks, each medication group was given relevant medicine for gavage. After 12 weeks, Hcy, TC, TG, HDL-C and LDL-C level in serum were detected, and abdominal aorta vascular ultrasound were observed.Results The model group was higher than the normal group in the level of Hcy (P<0.05). Compared with the model group, the levels of Hcy in Angelicae Sinensis Radix volatileoil group, the ferulic acid group and the folic acid group decreased (P<0.05). Compared with the model group, the levels of TC, TG and LDL-C in AngelicaeSinensis Radix volatile oil group, ferulic acid group and folic acid group decreased (P<0.05). Abdominal aorta ultrasonography showed that the degree of AS was significantly attenuated in folic acid group, Angelicae Sinensis Radix volatile oil group and ferulic acid group.Conclusion Angelicae Sinensis Radix volatile oil and ferulic acid in Min Angelicae Sinensis Radix can reduce the harm to endothelium and can prevent and treat HHcy caused by rabbit AS.
RESUMEN
Objective To investigate the protective effects of Angelicae Sinensis Radix and Angelicae Sinensis Radix polysaccharides on immune function injury induced by X rays in SD rats. Methods Forty SD rats were randomly divided into control group, model group, Angelicae Sinensis Radix Decoction group, Angelicae Sinensis Radix polysaccharide group and positive medicine group. After routine feeding for 14 day, each administration group was given relevant medicine for gavage, while control group and model group were given the same amount of distilled water for gavage, once a day for 7 d. From the 8th day, except for the control group, the rats in the rest of groups were subjected to whole-body X ray irradiation, continuous exposure to 2 d; the total absorbed dose was 6 Gy. The rats were killed by femoral artery after irradiate 3 d. The WBC count, RBC, HGB, and PLT in peripheral blood were observed by blood routine test; the number of nucleated cells in the bone marrow was observed by nucleated cell count method; the pathological changes of spleen were observed by HE staining under microscope; the contents of IFN-γ and IL-4 in serum were detected by ELISA. Results Compared with the control group, the spleen index WBC, number of bone marrow nucleated cells and serum contents of IL-4 and IFN-γ in model group was significantly lower (P<0.05), The contents of RBC and HGB increased (P<0.05). Compared with the model group, WBC, number of bone marrow nucleated cells, and the contents of IL-4 and IFN-γ in serum of each administration group increased significantly (P<0.05); RBC and HGB decreased significantly (P<0.05). Conclusion Angelicae Sinensis Radix and Angelicae Sinensis Radix polysaccharides have protective effects on the immune function injure induced by X ray in SD rats.