Effect of ATP50 on GH-induced testosterone secretion in isolated rat Leydig cells / 重庆医科大学学报

Objective:Toinvestigate the effect of ATP50 on GH-induced testosterone secretion in isolated SD rat Leydigcells.Methods:The Leydig cells were treated with ATP50 antisense oligodeoxynucletides(Anti-ODN)in GH group,nonsense tat ODN in control group,cAMP and testosterone in culture medium was detected by radioimmunoassay,expression of ATP50 protein was investigated by immunohistochemistry.Results:Antisense ATP50 ODN significantly inhibited GH-induced testosterone secretion of isolated rat Leydig cells in a dose-dependent manner(P

The study on different acting mechanisms of three types of TACE inhibitors in converting of pro-TNF? into sTNF? / 中国免疫学杂志

Objective:To study the effects of TNF? converting enzyme(TACE)inhibitors on TNF? secretion and develop an approach to interfere inflammation processes.Methods:(1)Stimulate the HL-60 cell lines in vitro with LPS for different time to establish the cellular model of inflammation and simultaneously induce in vivo inflammatoin animal model by LPS.(2)Check the cytotoxic effects of TNF? secretion using MTT colorimetric method for cell proliferaton.(3)Detect the level of expression of TACE carrying out RT-PCR,FCM techniques and immuno-histochemical dying technique.Results:(1)PDQ had the inhibitive effect on TNF?mRNA expression induced by LPS stimulation( P