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Objective To investigate the effects of different detection methods on the results for alanine aminotransferase (ALT),HBsAg and hepatitis C virus antibody (HCV-Ab) through testing the blood of 273 316 person time blood donors from 2009 to June2015,and to investigate the distribution of HBsAg,HCV-Ab and ALT and their correlation in blood donors,detection mode of nucleic acid detection in negative samples used enzyme-linked immunosorbent assay (ELISA),the results of blood donors,who were single reagent HBsAg,HCV-Ab positive,were detected again after for 24 weeks (maximum window period),and necessity of ALT screening before collecting blood sample,which provided a basis for the optimization of blood detection methods.Methods The rate method was used to detect ALT,detection of HCV-Ab and HBsAg was used ELISA,nucleic acid detection was performed on HBsAg and HCV-Ab negative blood by enzyme immunoassay,and dry chemistry complete automatic biochemistry analyzer were used for ALT screening before blood sampling in street.The enumeration data were analyzed by the x2 test with P ＜ 0.05 for statistically significant.Results The total ALT positive rate of 273 316 people was 4.09％ in Baoji city,the single ALT positive was main (98.17％,11 185/273 316).The ALT positive rate (5.20％) of male was higher than that of women (1.79％) (x2 =1780.94,P ＜0.05),and the ALT positive rate of 18 ～44 years old group (4.48％) was higher than that of 45 ～ 55 years old groups (3.26％) (x2 =223.87,P ＜ 0.05).The positive rate of ALT before carting out ALT screening (4.90％,8 448/172 472) was higher than that of after carrying ALT screening (2.71％,2 737/100 844) (x2 =773.43,P ＜ 0.05).Through nucleic acid detection for serums of 143 815 ELISA negative blood donors,there were no HCV-Ab positive and 75 HbsAg positive.The Kappa value was 0.049 between ELISA and nucleic acid examination in single reagent HBsAg positive blood donors after 24 weeks.Conclusions The ALT positive and HBV,HCV had no correlation,and the ALT single positive were main in blood donors.Carrying out ATL blood screening,two times of ELISA detection and one times the nucleic acid detection may improve the blood safety level.HBsAg and HCV-Ab positive blood donors in 24 weeks (maximum window period) after ELISA and nucleic acid detection can be negative.Return blood donation team.The HbsAg and HCV-Ab positive blood donors,who were negative by the ELISA and nucleic acid detection after the 24 week (maximum window period),can return to the blood donation team.
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Objective To study the clinical value of wrist magnetic resonance imaging (MRI) combined anti-cyclic citrullinated peptide antibody detection in the diagnosis of early rheumatoid arthritis (RA).Methods Forty five patients with early RA were selected as RA group,45 cases of patients without rheumatoid arthritis as non-RA group,and 43 cases of people with normal examination as control group.All subjects were given wrist MRI and anti-cyclic citrullinated peptide (CCP) antibody with the enzyme linked immunosorbent assay (ELISA).At the same time,clinical symptoms,physical signs,MRI manifestations,and laboratory indicators were collected.All results were statistically analyzed.Results Positive rate of MRI lesions and serum anti-CCP antibody in RA group were significantly higher than non-RA group and control group (P ＜0.05).The sensitivity and specificity of MRI (or anti-CCP antibody) for early RA were 88.88％ and 82.22％ (or 68.88％ and 91.11％).The sensitivity (64.44％) of MRI combined with anti-CCP antibody was decreased compared to individual; however,the specificity (100％) of MRI combined with anti-CCP antibody was increased.The correlation of MRI synovial scores and anti-CCP antibody levels was positively correlated (rs =0.612,P ＜ 0.05).MRI abnormal signs and joint disease activity score (DAS28) were positively correlated (rs =0.521,P ＜ 0.05).Anti-CCP antibody levels and DAS28 were positively correlated (rs =0.541,P ＜ 0.05).Conclusions MRI examination and combined with anti-CCP antibody detection is helpful to improve the diagnosis of early RA,and it provides a detection basis for dynamic assessment of RA condition changes.