RESUMEN
Objective To investigate the antiviral activity and chemical composition of n-butanol extract of Isatidis Radix. Methods Each component of total n-butanol extracts was detected by solvent and chromatogram, the anti-HSV-1 activities of each component were detected by MTT assay in vitro, each component of total n-butanol extracts was analyzed by CUPLC-Q-TOF-MS, and the data were analyzed by Markview and Excel software to find the difference among different components. According to statistics, the components related to antivirus activities were obtained. Results According to the similarity of chemical composition, 20 components were extracted and separated from n-butanol extract of Isatidis Radix by chromatography, and 10 components had antiviral activity. Forty-four chemical components were detected by UPLC-Q-TOF-MS from n-butanol extracts and their chemical structures were confirmed. Difference analysis of chemical components discovered 15 chemical components such as alkaloids, organic acids, sugars and amino acid derivatives associated with antiviral activity, especially 1-methoxy-3-carbaldehyde, 3-(3’,5’-dimethoxy- 4’-hydroxy)-2-indolinone, 1-methoxy-3-indolinone, 3-(methylsulfinyl) propylglucosinolate, and glucosamine-6-phosphatee in higher levels of the active components, which was worth further study. Conclusion The n-butanol extract of Isatidis Radix mainly contains indole alkaloids, organic acids, nucleosides and amino acids, wherein the single-indole, a sulfur glycosides and sugars and amino acid derivatives are closely related to the antiviral activity.
RESUMEN
3200 mg?L~(-1)),and could suppress HBeAg and HBsAg secretion and lower the HBV-DNA level cultured in HepG2 2.2.15 cell line in a dose and time-dependent manner.Conclusion Some of the mechanisms of PSP against HSV-1 and HSV-2 may be explained by the considerable inhibition of virus absorption and virus replication in cells.Some of the mechanisms of PSP against HBV may be explained by inhibition of the secretion of HBV-antigen and the replication of HBV-DNA.
RESUMEN
Objective To extract the sulfated polysaccharides from Grateloupia turuturu Yamada and to studyits physico-chemical properties and antiviral activities.Methods The sulfated polysaccharides were extracted from Grateloupia turuturu Yamada by hot water extraction and alcohol precipitation.Two pure polysaccharides were obtained by DEAE-52 cellulose column chromatography.The physico-chemical properties including the composition of the glycoconjugate and the content of the sulfate were analyzed.The antiviral effects of the samples on HSV-1,Cox_(3) and their cytotoxicity to Vero,Hela cell were studied by cytopathic effect(CPE) reduction assay and MTT colorimetric assay.Results The results showed that the glycoconjugate was composed of Gal and slight Glc,Xyl etc.The content of the sulfate group was more than 15%.And the polysaccharides showed good antiviral activities,especially the pure polysaccharides.Their 50% inhibited concentrations(IC_(50))on HSV-1 were(7.81) and 3.91?g?mL~(-1),selectivity index(SI)values were larger than 128 and 256,respectively.Conclusion The sulfated polysaccharides from Grateloupia turuturu Yamada have good antiviral effects on HSV-1 and Cox_(3).